Effect of Hizikia fusiformis extracts on reactive oxygen species mediated oxidative damage

Abstract

We investigated the effect of Hizikia fusiformis extracts on the production of cellular reactive oxygen species (ROS) in human fibroblast cell line HT-1080to evaluate their antioxidant activities. The H 2 O 2 -induced ROS generation was measured using a dichlorofluorescein-diacetate (DCFH-DA) assay. The acetone+methylene chloride (A+M)and methanol (MeOH) extracts dosedependently decreased the level of ROS production induced by H 2 O 2 in comparison with the levels observed in the control without the extracts. Treatments with A+M and MeOH extracts for 120 min reduced ROS generation by 86% and 77%, respectively. All tested fractions decreased ROS production in a concentration-dependent manner. Treatments with n-hexane,85% aqueous MeOH and n-butanol (BuOH) fractions resulted in54%, 54% and 31% ROS inhibition, respectively. These results indicate that both A+M and MeOH extracts of H. fusiformis had a stronger effect in reducing ROS production. This present preliminary study suggests that extracts from H. fusiformis have nutraceutical value with potent antioxidant activity that alleviates radical-induced damage.

In Vitro and In Vivo Antitumor Efficacy of Hizikia fusiforme Celluclast Extract against Bladder Cancer

“…Monosaccharide composition assays revealed a higher amount of fucose in SPHF than in CHF. Previous studies have demonstrated that high levels of sulfated polysaccharide and fucose are linked to stronger antioxidant activities [ 20 , 23 , 31 ]. Additionally, the sulfating process has been found to enhance the physiological bioactive effects of polysaccharides [ 20 , 31 ].…”

“…Hizikia fusiforme (HF) is a widely-known edible brown seaweed that contains polysaccharides, vitamins, and phenolic compounds, all of which are associated with numerous physiological benefits [20][21][22][23][24][25][26][27]. Particularly, polysaccharide-enriched HF has recently attracted attention due to its diverse therapeutic properties [20][21][22][23][24][25][26][27]. Previous studies have demonstrated the antitumor effect of polysaccharide-enriched HF in vitro [26,27].…”

“…However, these byproducts contain several types of sulfated polysaccharides [ 21 ], which have been found to possess various beneficial properties [ 20 , 21 ]. Previous studies have revealed that HF polysaccharides possess antioxidant, anti-inflammatory, anti-diabetic, osteoprotective, and immunostimulatory effects [ 21 , 22 , 23 , 24 , 25 ]. Notably, a recent report demonstrated that sulfated polysaccharides extracted from acid-processed HF possessed antitumor effects in vitro [ 26 , 27 ].…”

Protective Effect of Sulfated Polysaccharides from Celluclast-Assisted Extract of Hizikia fusiforme Against Ultraviolet B-Induced Skin Damage by Regulating NF-κB, AP-1, and MAPKs Signaling Pathways In Vitro in Human Dermal Fibroblasts

“…It contains various compounds, which possesses several of bioactivities, especially polysaccharides. Many studies have reported that polysaccharides from H. fusiforme possess various bioactivities such as antioxidant, anti-inflammatory, anti-angiogenic, anticancer, osteoprotective, and immunostimulatory activities [ 21 , 22 , 23 , 24 ]. Our previous research displayed that sulfated polysaccharides from Celluclast-assisted extract of H. fusiforme (HFPS) possess strong free radical scavenging activity and protective effects on H 2 O 2 -induced oxidative stress in vitro in Vero cells and in vivo in zebrafish [ 25 ].…”