Protective Effect of Sulfated Polysaccharides from Celluclast-Assisted Extract of Hizikia fusiforme Against Ultraviolet B-Induced Skin Damage by Regulating NF-κB, AP-1, and MAPKs Signaling Pathways In Vitro in Human Dermal Fibroblasts

Abstract

Our previous study evaluated the antioxidant activities of sulfated polysaccharides from Celluclast-assisted extract of Hizikia fusiforme (HFPS) in vitro in Vero cells and in vivo in zebrafish. The results showed that HFPS possesses strong antioxidant activity and suggested the potential photo-protective activities of HFPS. Hence, in the present study, we investigated the protective effects of HFPS against ultraviolet (UV) B-induced skin damage in vitro in human dermal fibroblasts (HDF cells). The results indicate that HFPS significantly reduced intracellular reactive oxygen species (ROS) level and improved the viability of UVB-irradiated HDF cells in a dose-dependent manner. Furthermore, HFPS significantly inhibited intracellular collagenase and elastase activities, remarkably protected collagen synthesis, and reduced matrix metalloproteinases (MMPs) expression by regulating nuclear factor kappa B (NF-κB), activator protein 1 (AP-1), and mitogen-activated protein kinases (MAPKs) signaling pathways in UVB-irradiated HDF cells. These results suggest that HFPS possesses strong UV protective effect, and can be a potential ingredient in the pharmaceutical and cosmetic industries.

Pholiota nameko Polysaccharides Promotes Cell Proliferation and Migration and Reduces ROS Content in H2O2-Induced L929 Cells

“…Inhibitory effect on collagenase was performed by modified Wang's method [27]. To measure the collagenase activity, 100 µL of 200 units/mL collagenase and 100 µL PNPs (5000, 2500, 1250 µg/mL) were mixed together and incubated at 37 • C for 15 min, weighed at 1 mg of azo dye-impregnated collagen substrate and mixed with 800 µL 0.1 M Tris-HCl buffer (pH 7.0), then added together at 43 • C for 1 h under shaking conditions.…”

Anti-Obesity Effects of Grateloupia elliptica, a Red Seaweed, in Mice with High-Fat Diet-Induced Obesity via Suppression of Adipogenic Factors in White Adipose Tissue and Increased Thermogenic Factors in Brown Adipose Tissue

“…Natural products, such as polyphenols, alkaloids, terpenoids, organosulfur compound, phytosterols, and bioactive compounds obtained from land-based and marine resources, were shown to exhibit anti-obesity effects through the regulation of obesity-related risk factors [23][24][25][26][27][28]. Marine seaweeds are a potential rich source of bioactive metabolites, including polyphenols, polysaccharides, and peptides, that exhibit antioxidant, anticancer, antidiabetic, anti-inflammation, anti-ultraviolet, and anti-obesity activities [29][30][31][32][33][34][35][36][37]. Many researchers have reported that marine seaweed metabolites, such as alginates, fucoidans, and phlorotannins, have potential anti-obesity effects in in vivo animal studies [38].…”

Protective effect of sulfated polysaccharides from a Celluclast-assisted extract of Hizikia fusiforme against ultraviolet B-induced photoaging in vitro in human keratinocytes and in vivo in zebrafish

“…It has been used as a food ingredient and herbal medicine for hundreds of years (Li et al 2006). H. fusiforme contains a particular high amount of polysaccharides that possess various bioactivities (Wang et al 2018a). In a previous study, we suggested that sulfated polysaccharides isolated from a Celluclast-assisted extract of H. fusiforme (HFPS) possess strong antioxidant activities in vitro and in vivo (Wang et al 2018b).…”