Effect of hydroxytyrosol on sperm post-thaw motion and velocity of cryopreserved ram semen

Balaganur, Krishnappa; Srivastava, S. K.; Kumar, Davendra; Ghosh, Subrata; Kalyan, DE; Paul, Rajani Kr.; Bahire, S.V.; Naqvi, S. M. K.

doi:10.5958/0973-9718.2018.00027.2

Cited by 17 publications

(16 citation statements)

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“…Results derived from the present study reported the fact that in samples supplemented with 30 µg/mL of HT an increase in progressive motility of 11% was reported in comparison to Control treatment which agrees the results reported by Hamden et al [22], who supplemented rat sperm with 50 µg/mL HT and those by Krishnappa et al [24] who reported an improvement in total motility when 80 mM HT was added to ovine sperm. These findings suggest that the presence of HT could mitigate ROS concentration, preventing the negative impact of moderately elevated ROS concentrations on the sperm movement, mostly via depletion of intracellular ATP and the successive reduction in the phosphorylation of axonemal proteins [53].…”

Section: Discussionsupporting

confidence: 92%

“…By contrast, there are few studies on the effects derived from the addition of these antioxidants to sperm dilution media in animals. Contextually, HT-supplemented sperm extender has previously been evaluated in studies conducted on rats [22], humans [23], and rams [15,24,25]. However, the effect of DHPG supplementation has only been reported in ram sperm [15,25].…”

Section: Introductionmentioning

confidence: 99%

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Bayesian Analysis of the Effects of Olive Oil-Derived Antioxidants on Cryopreserved Buck Sperm Parameters

Arbulu

González

Bermúdez-Oria

et al. 2021

Animals

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The present study evaluates the effect of olive oil-derived antioxidants, hydroxytyrosol (HT) and 3,4-dihydroxyphenylglycol (DHPG), on cryopreserved caprine sperm using Bayesian inference of ANOVA. For this proposal, sperm was collected, pooled and diluted in freezing media supplemented with different concentrations of HT, DHPG and the mixture (MIX) of both antioxidants. Sperm motility, viability, acrosome integrity, mitochondrial status, and lipid peroxidation (LPO) were assessed in fresh and frozen-thawed sperm samples. The results provided evidence that HT at low concentrations improves sperm motility and viability, and reduces the LPO. Contrastingly, DHPG and MIX exert a positive effect by reducing LPO values as concentration increases. Additionally, mitochondrial potential was reduced when samples were supplemented with HT at low concentrations and mixture of both antioxidants. Conclusively, the addition of olive oil-derived antioxidants (HT at 10 µg/mL and DHPG at 30 µg/mL) implements a protective effect in cryopreserved buck sperm. Bayesian analysis alternatives offer new possibilities to determine the repercussion of antioxidants on sperm, both quantitatively and qualitatively.

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Section: Discussionsupporting

confidence: 92%

Section: Introductionmentioning

confidence: 99%

Bayesian Analysis of the Effects of Olive Oil-Derived Antioxidants on Cryopreserved Buck Sperm Parameters

Arbulu

González

Bermúdez-Oria

et al. 2021

Animals

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“…In the present study, the addition of different antioxidant types and concentrations did not significantly improve sperm TM and PM for both studied temperatures, according with our previous results obtained in cryopreserved samples, where no significant effects were observed for TM and PM when HT, DHPG and the combination of both were used (Arando et al, 2019). By contrast, the addition of 80 µM HT has recently demonstrated a positive effect on the postthawed sperm motility in ram sperm (Balaganur et al, 2018). Hamden et al (2010 observed that rat sperm samples previously incubated with high concentrations of glucose (that is toxic for spermatozoa)…”

Section: Discussionsupporting

confidence: 88%

“…In our knowledge, few studies evaluate the effect of olive oil on the sperm quality. Al-Daraji (2012) studied the effect of the direct addition of olive oil into the extender media in rooster sperm, while others evaluated the effect of HT-based extender on rat (Hamden, Allouche, Damak, Carreau, & Elfeki, 2010), human (Kedechi et al, 2017) and ram sperm (Arando et al, 2019;Balaganur et al, 2018).…”

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confidence: 99%

Effect of olive‐derived antioxidants (3,4‐dihydroxyphenylethanol and 3,4 dihydroxyphenylglycol) on sperm motility and fertility in liquid ram sperm stored at 15°C or 5°C

Arbulu

Delgado

Bermúdez-Oria

et al. 2020

Reprod Domestic Animals

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The aim of the present study was to evaluate the effect of the addition of two olive oil‐derived antioxidants, hydroxytyrosol (3,4‐dihydroxyphenylethanol, HT) and 3,4‐dihydroxyphenylglycol (DHPG), on ovine semen during liquid storage at 5°C and 15°C. Semen was collected, pooled, diluted and then divided into aliquots supplemented with different concentrations (5 μg/ml, 10 μg/ml, 50 μg/ml and 100 μg/ml) of HT, DHPG and a mixture (MIX) of both antioxidants. Sperm motility characteristics were assessed in the different samples at 0, 6, 24, 48, 72 and 96 hr after cooling, and a fertility trial was also conducted. The results showed that the antioxidant addition did not significantly improve total and progressive motility in ovine cooled sperm maintained at 15° or 5°C. However, in samples stored at 5°C, LIN (48, 72, 96 hr), STR (0 hr) and WOB (0, 48, 72, 96 hr) values significantly decreased in comparison with control treatment when high antioxidant concentrations were added (MIX100 or HT100). When samples were maintained at 15°C, MIX50 showed significantly higher VCL values than the control treatment after 6 hr cooling, and MIX100 showed significantly lower VCL values at 96 hr after cooling. According to the artificial insemination trial, no significant differences were observed when antioxidants were added. In conclusion, the use of HT and DHPG showed small impact in sperm motility and fertility was not affected (nor detrimentally nor positively) when insemination was carried out using antioxidant‐supplemented liquid sperm.

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“…For instance, HT can protect vascular endothelial cells from the cytotoxic effects of hydrogen peroxide [33]. To the present, the reports of HT in sperm are only in the cryopreservation of ram sperm [3,19] and human sperm antioxidant capacity [16]. There is still no research to evaluate the effect of HT in the preservation of pig sperm at 17℃.…”

Section: Introductionmentioning

confidence: 99%

Hydroxytyrosol Effectively Improves The Quality of Pig Sperm at 17 °C

Liu

Zhang

Tian

et al. 2021

Preprint

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Background: Artificial insemination technology is the production technology used in more than 99% of the world's pig farms. As the frozen sperm damage is very serious, the preservation of pig sperm is mainly carried out at 17°C. However, the accumulation of a large amount of reactive oxygen species (ROS) during the preservation process is the main reason for the deterioration of sperm quality. In our research, by adding different concentrations of hydroxytyrosol to the diluent during the storage of pig sperm at 17°C, we compared them with the traditional diluent to study the cumulative amount of ROS, the extent of membrane damage, the sperm acrosome integrity, the activity of antioxidant enzymes, and other indicators to evaluate the effect of hydroxytyrosol on the changes of active oxygen species and sperm quality during storage. After we determined the optimal amount of hydroxytyrosol in the diluent, proteomics was used to monitor difference in sperm protein expression between the control group and the addition of 120 μmol/L hydroxytyrosol group (optimum concentration). Ultimately, we selected the control group and the 120 μmol/L hydroxytyrosol group to test the effect of artificial insemination.Results: The results of our research showed that during storage of pig sperm at 17 ℃, the sperm quality and antioxidant capacity of the hydroxytyrosol-treated group were significantly improved (P < 0.05). Proteomics sequencing analysis proved that the addition of hydroxytyrosol treatment group has potential value in improving sperm quality. Besides, the significant increase in sow pregnancy rate and piglet birth weight also confirmed that sperm quality during storage can be of vital importance in actual production (P < 0.05).Conclusions: Based on our results, we demonstrate that the addition of hydroxytyrosol to the diluent can improve the quality of pig sperm and the efficiency of artificial insemination.

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Effect of hydroxytyrosol on sperm post-thaw motion and velocity of cryopreserved ram semen

Cited by 17 publications

References 0 publications

Bayesian Analysis of the Effects of Olive Oil-Derived Antioxidants on Cryopreserved Buck Sperm Parameters

Bayesian Analysis of the Effects of Olive Oil-Derived Antioxidants on Cryopreserved Buck Sperm Parameters

Effect of olive‐derived antioxidants (3,4‐dihydroxyphenylethanol and 3,4 dihydroxyphenylglycol) on sperm motility and fertility in liquid ram sperm stored at 15°C or 5°C

Hydroxytyrosol Effectively Improves The Quality of Pig Sperm at 17 °C

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