Effect of incubation conditions, inhibitors and seminal plasma on protein synthesis in ram spermatozoa

Ahmed, Nikhat; Salem, M. H.; El-Oksh, H.A.; Pursel, V. G.

doi:10.1530/jrf.0.0710213

Cited by 11 publications

(4 citation statements)

References 13 publications

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“…Incorporation of Se into the seleno protein fraction of the mouse, rat, ram, and bull spermatozoa seems to be completed during the early maturation phase of spermatogenesis (Gunn et al, 1967;Gunn and Gould, 1970;Smith et al, 1979;Pond et al, 1983; al., 1987). Mitochondrial protein synthesis in ejaculated spermatozoa has been noted in bulls (Premkumar and Bhargava, 1972), humans (Mujica, 1976), mice (Bragg and Handel, 1979), and rams (Ahmed et al, 1984). Our study supports the concept that Se is incorporated into the functional selenoproteins of the spermatozoa midpiece during early spermatogenesis.…”

Section: Discussionsupporting

confidence: 85%

Effect of dietary selenium and vitamin E on the ultrastructure and ATP concentration of boar spermatozoa, and the efficacy of added sodium selenite in extended semen on sperm motility.

Marin-Guzman

Mahan

Whitmoyer

2000

Journal of Animal Science

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Three experiments evaluated the effects of dietary Se and vitamin E on the ultrastructure of spermatozoa, ATP concentration of spermatozoa, and the effects of adding sodium selenite to semen extenders on subsequent sperm motility. The experiment was a 2 x 2 arrangement of treatments in a randomized complete block design. A total of 10 mature boars were fed from weaning to 18 mo of age diets fortified with two levels of supplemental Se (0 or .5 ppm) or vitamin E (0 or 220 IU/kg diet). The nonfortified diets contained .06 ppm Se and 4.4 IU vitamin E/kg. In Exp. 1, the spermatozoa from all boars were examined by electron microscopy. Vitamin E had no effect on structural abnormalities in the spermatozoa. When the low-Se diet was fed the acrosome or nuclei of the spermatozoa was unaffected, but the mitochondria in the tail midpiece were more oval with wider gaps between organelles. The plasma membrane connection to the tail midpiece was not tightly bound as when boars were fed Se. Immature spermatozoa with cytoplasmic droplets were more numerous when boars were fed the low-Se diet, but the occurrence of midpiece abnormalities occurred in boars fed diets with or without Se or vitamin E. Our results suggest that Se may enhance spermatozoa maturation in the epididymis and may reduce the number of sperm with cytoplasmic droplets. In Exp. 2, the concentration of ATP in the spermatozoa was evaluated in the semen of all treatment boars. When the low-Se diet was fed, ATP concentration was lower (P < .01), whereas vitamin E had no effect on ATP concentration. Experiment 3 investigated the effect of diluting boar semen with a semen extender with sodium selenite added at 0, .3, .6, or .9 ppm Se. Three ejaculates from each boar were used to evaluate these effects on sperm motility to 48 h after dilution. Sperm motility declined (P < .01) when Se was added to the extender, and this decline was exacerbated as the concentration of added Se increased (P < .01). The added Se was demonstrated to be tightly adhered to the spermatozoa. Overall, these results suggest that low Se-diets fed to boars resulted in abnormal spermatozoal mitochondria, a lower ATP concentration in the spermatozoa, and a loose apposition of the plasma membrane to the helical coil of the tail midpiece, but no effect from inadequate vitamin E was demonstrated. Adding sodium selenite to the semen extender reduced sperm cell motility.

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Section: Discussionsupporting

confidence: 85%

Effect of dietary selenium and vitamin E on the ultrastructure and ATP concentration of boar spermatozoa, and the efficacy of added sodium selenite in extended semen on sperm motility.

Marin-Guzman

Mahan

Whitmoyer

2000

Journal of Animal Science

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“…Previous studies reported RNA and/or protein synthesis in spermatozoa of several mammalian species: bull [11,12], human [13], mouse [16] and ram [14,15]. In spite of the precautions taken by the investigators to prevent bacterial contamination, we have shown that they are insufficient to avoid bacterial protein synthesis.…”

Section: Discussionmentioning

confidence: 68%

“…In addition, a fully differentiated spermatozoon has shut down most of its biosynthetic capacity [10] and therefore it is of interest to determine how mtDNA expression is adapted to this situation and, in particular, if mitochondria maintain their synthetic protein activity after ejaculation. An early report proposed an active mitochondrial protein synthesis activity in ejaculated bull sperm [11,12]; additional evidence supported this observation in a variety of mammalian species: human [13], ram [14,15] and mouse [16]. Therefore, an asynchrony between the expression of nuclear and mtDNA OXPHOS‐encoded genes could potentially occur in spermatozoa.…”

Section: Introductionmentioning

confidence: 88%

Mitochondria from ejaculated human spermatozoa do not synthesize proteins

et al. 2003

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Sperm motility is dependent on mitochondrial ATP production that relies on the coordinated expression of the mitochondrial and nuclear genomes. It is generally accepted that mammalian ejaculated spermatozoa retain the ability to synthesize mtDNA-encoded proteins but not most of the nuclear ones. This implies an asynchronous regulation of the oxidative phosphorylation-related genes encoded by each genome. Trying to investigate this issue, we unexpectedly found that ejaculated human spermatozoa do not synthesize mtDNA-encoded proteins. Moreover, we estimated that the discrepancy between our observations and those published elsewhere was due to a chloramphenicol-sensitive protein synthesis attributed to mitochondria that instead corresponds to contaminating bacteria.

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“…Mitochondrial ribosomes seem to be responsible for de novo protein synthesis during capacitation as sperm lacks classical cytoplasmic machinery ( Ostermeier et al ., 2002 ; Gilbert et al ., 2007 ). Accordingly, several publications have highlighted that cycloheximide (cytoplasmic ribosome inhibitor) did not affect sperm protein synthesis ( Bragg and Handel 1979 ; Ahmed et al ., 1984 ; Naz 1998 ; Gur and Breitbart 2006 ).…”

Section: Capacitation Increases the Content Of Atp1a4 In Bovine Sperm...mentioning

confidence: 99%

Na/K-ATPase and Regulation of Sperm Function

Thundathil¹,

Rajamanickam²,

Kastelic³

2018

Anim Reprod

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A standard bull breeding soundness evaluation (BBSE) identifies bulls with semen that is grossly abnormal. Nonetheless, semen samples classified as satisfactory based on these traditional approaches differ in fertility; perhaps there are submicroscopic differences in sperm characteristics affecting fertility. Therefore, a better understanding of molecular regulation of sperm function could promote development of novel, evidence-based approaches to predict male fertility. Recently the α4 isoform of Na/K-ATPase (ATP1A4) has received considerable attention, due to its testisspecific expression in post-meiotic germ cells and mature sperm, in addition to its regulation of sperm motility and capacitation. Using fresh bull sperm, we determined that ATP1A4 resided in specialized microdomains (raft and non-raft) of the sperm plasma membrane and activated specific signaling (caveolin-1, EGFR, Src, ERK1/2) molecules during sperm capacitation. Furthermore, ATP1A4 was the predominant isoform responsible for total Na/K-ATPase activity in capacitated sperm. Despite the widely accepted dogma of transcriptional/translational quiescence, bovine sperm translated ATP1A4 mRNA on mitochondrial or mitochondrial-type ribosomes, increasing their content and activity during capacitation. Proteomic analysis of raft and non-raft fractions revealed a significant interaction between ATP1A4 and plakoglobin, a member of the β-catenin family of proteins involved in cell adhesion, in the equatorial segment of capacitated sperm, suggesting a potential role in sperm-oolemma fusion. In frozen-thawed sperm, ATP1A4 content and activity was greater in high-versus low-fertility bulls. Additionally, ATP1A4-induced increases in ROS, calcium, actin polymerization and tyrosine phosphorylation were also involved in regulating post-thaw sperm function in these bulls. Overall, results demonstrated that ATP1A4 had unique roles in controlling several aspects of sperm physiology, acting through well-established enzyme activity and signaling functions. Consequently, isoforms of Na/K-ATPase are potential biomarkers for male fertility.

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Effect of incubation conditions, inhibitors and seminal plasma on protein synthesis in ram spermatozoa

Abstract: Summary. The effects of incubation time (15 min\p=n-\4h)

Cited by 11 publications

References 13 publications

Effect of dietary selenium and vitamin E on the ultrastructure and ATP concentration of boar spermatozoa, and the efficacy of added sodium selenite in extended semen on sperm motility.

Effect of dietary selenium and vitamin E on the ultrastructure and ATP concentration of boar spermatozoa, and the efficacy of added sodium selenite in extended semen on sperm motility.

Mitochondria from ejaculated human spermatozoa do not synthesize proteins

Na/K-ATPase and Regulation of Sperm Function

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