Effect of interleukin 8 and ICAM-1 on calcium-dependent outflow of K<sup>+</sup> in erythrocytes from subjects with essential hypertension

Buemi, Michele; Marino, Domenico; Floccari, Fulvio; Ruello, Antonella; Nostro, Lorena; Aloisi, Carmela; Marino, Marco; Pasquale, G. Di; Corica, Francesco; Frisina, N

doi:10.1185/030079903125002720

Cited by 31 publications

(19 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…IL-6 enhances calcium-dependent potassium efflux and increases membrane permeability to calcium in red blood cells of hypertensive patients. These changes result in increased calcium influx, and finally in elevated BP [19]. However, it remains unclear as to whether IL-6 enhances potassium efflux and calcium influx in T lymphocytes of hypertensive patients.…”

Section: Discussionmentioning

confidence: 99%

Potassium channel changes of peripheral blood T-lymphocytes from Kazakh hypertensive patients in Northwest China and the inhibition effect towards potassium channels by telmisartan

et al. 2016

View full text Add to dashboard Cite

A b s t r a c tBackground and aim: Increasing evidence indicates that chronic inflammation is a direct or indirect manifestation of hypertension. Potassium channels are thought to be critical for lymphocyte activation, which suggests that hypertension may be an inflammatory disease initiated at the ion channel level. Methods:This study investigated changes in interleukin (IL)-6, IL-17, and transforming growth factor beta (TGF-b1) expression in the blood of Kazakh hypertensive patients in Northwest China using ELISA technology. Whole-cell patch clamp technology was used to evaluate current changes associated with Kv1.3 and KCa3.1 in peripheral blood T lymphocytes of hypertensive patients, and to investigate current changes induced by telmisartan. We also investigated the effects of telmisartan on expression of Kv1.3 and KCa3.1 at mRNA and protein levels in peripheral blood T lymphocytes using real-time polymerase chain reaction and Western blot analysis. Results:Expression of IL-6, IL-17 and TGF-b1 in the blood of Kazakh hypertensive patients in Northwest China was significantly higher than in healthy controls (p < 0.05). The current mediated by Kv1.3 and KCa3.1 and the corresponding expression at mRNA and protein levels in T lymphocytes were also higher in these hypertensive patients than in controls (p < 0.05). Telmisartan intervention for 24 h and 48 h inhibited the current and expression of Kv1.3 and KCa3.1 at mRNA and protein levels (p < 0.05). Conclusions:These results indicated that the increase in functional Kv1.3 and KCa3.1 channels expressed in T lymphocytes of Kazakh patients with hypertension was blocked by telmisartan, resulting in a reduced inflammatory response. These results provide theoretical support for the treatment of hypertension at the cellular ion channel level.

show abstract

Section: Discussionmentioning

confidence: 99%

Potassium channel changes of peripheral blood T-lymphocytes from Kazakh hypertensive patients in Northwest China and the inhibition effect towards potassium channels by telmisartan

et al. 2016

View full text Add to dashboard Cite

show abstract

“…The increased inward flow of Ca 2+ leads to the contraction of vascular smooth muscular cells (VSMC) which escalates BP. 22 Circulating TNF-a, directly or indirectly, stimulates the abnormal expression through endothelin from endothelial cells of proto-oncogene such as c-sis, c-myc, and c-fos that may promote the synthesis of DNA and the proliferation of VSMC, thus leading to the thickening of vascular wall, narrowing of lumen, increasing of peripheral resistance, and finally promoting of BP. The study by Azra Mahmud et al 23 also demonstrated that TNF-a and IL-6 were related to arterial stiffness.…”

Section: Discussionmentioning

confidence: 99%

Correlation of tumor necrosis factor alpha and interleukin 6 with hypertensive renal damage

Yang

2010

Renal Failure

View full text Add to dashboard Cite

Background: Many efforts have been made to investigate the role played by cytokines in the development of hypertension. But few reports exist on the association between cytokines and hypertensive renal damage. This study was to observe the changes of the serum levels of cytokines, tumor necrosis factor alpha (TNF-a), and interleukin 6 (IL-6) in patients with hypertensive renal damage, whereby to study the correlation of TNF-a and IL-6 with the hypertensive renal damage. Methods: According to their urinary albumin excretion rate (UAER), 102 patients with essential hypertension were divided into three groups: normoalbuminuria hypertensive group (n = 37), microalbuminuria hypertensive group (n = 36), and proteinuric hypertensive group (n = 29). Serum TNF-a and IL-6 of all subjects were measured with radioimmune assay. Thirty age-and gendermatched normotensive persons were selected as normotensive control group. Results: Serum levels of TNF-a and IL-6 were significantly higher in patients with essential hypertension than those in normotensive control group (p < 0.5). Serum levels of TNF-a and IL-6 increased in proportion to UAER. The statistical significance was present among groups (p < 0.05). Both TNF-a and IL-6 were found to have a positive correlation with UAER (r = 0.79, p < 0.01; r = 0.75, p < 0.01), but not with the levels of blood pressure (BP). Conclusions: TNF-a and IL-6 are remarkably increased in hypertensive patients and may play an important role in the pathogenesis and the development of hypertensive renal damage.

show abstract

“…hMPV is reported to affect airway epithelial cells and stimulate massive production of interleukin-8, 30,31 and regulate upon activation normal T-cell expressed and secreted. 31 On the other hand, interleukin-8 is known to closely relate to the genesis of hypertension because it enhances membrane permeability to Ca 2+ and induces vasoconstriction in smooth muscle cells 32 and because an antihypertensive calcium channel blocker, azelnidipine, is reported to reduce circulating interleukin-8 levels. 33 Moreover, circulating interleukin-8 is known to predict the development of atherosclerosis in coronary arteries.…”

Section: Discussionmentioning

confidence: 99%

Association between human metapneumovirus seroprevalence and hypertension in elderly subjects in a long-term care facility

et al. 2011

View full text Add to dashboard Cite

Recently, relations between hypertension and infections caused by several pathogens have been reported. However, few studies have examined the relationship between human metapneumovirus (hMPV) and hypertension in elderly inpatients. To assess the association between anti-hMPV-immunoglobulin G (IgG) titer and the prevalence of hypertension, we conducted a case-control study in a Japanese long-term care facility (LTCF). The participants included 84 hypertensive patients aged X65 years, and 84 age-and sex-matched normotensive controls (38 males and 46 females in each group; cases, 79.9 ± 8.4 (s.d.) years; controls, 80.1 ± 8.3 years). Data on underling chronic clinical conditions were collected. Titers were measured using an immunofluorescence assay kit. The significance of risk factor differences was analyzed using univariate and multivariate comparisons of cases and controls. All serum samples were positive for hMPV, and IgG titers ranged from 40-fold to more than 5120-fold. There were no significant sex-or age-related differences in log 2 (anti-hMPV-IgG titer/10) among the subjects. Compared with normotensive subjects, hypertensive patients presented significantly higher log 2 (anti-hMPV-IgG titer/10) values (Po0.001). After adjustment with multiple logistic analysis, the odds ratio for log 2 (anti-hMPV-IgG titer/10) was 1.42 (95% confidence interval 1.16-1.75, P¼0.001) relative to normotensive subjects. In all subjects, stepwise multiple regression analysis revealed that both hypertension and a poor nutritional state independently contributed to increased log 2 (anti-hMPV-IgG titer/10). These observations suggest that an increased anti-hMPV-IgG titer was closely related to hypertension in elderly subjects in a Japanese LTCF.

show abstract

Effect of interleukin 8 and ICAM-1 on calcium-dependent outflow of K⁺ in erythrocytes from subjects with essential hypertension

Cited by 31 publications

References 20 publications

Potassium channel changes of peripheral blood T-lymphocytes from Kazakh hypertensive patients in Northwest China and the inhibition effect towards potassium channels by telmisartan

Potassium channel changes of peripheral blood T-lymphocytes from Kazakh hypertensive patients in Northwest China and the inhibition effect towards potassium channels by telmisartan

Correlation of tumor necrosis factor alpha and interleukin 6 with hypertensive renal damage

Association between human metapneumovirus seroprevalence and hypertension in elderly subjects in a long-term care facility

Contact Info

Product

Resources

About

Effect of interleukin 8 and ICAM-1 on calcium-dependent outflow of K+ in erythrocytes from subjects with essential hypertension

Cited by 31 publications

References 20 publications

Potassium channel changes of peripheral blood T-lymphocytes from Kazakh hypertensive patients in Northwest China and the inhibition effect towards potassium channels by telmisartan

Potassium channel changes of peripheral blood T-lymphocytes from Kazakh hypertensive patients in Northwest China and the inhibition effect towards potassium channels by telmisartan

Correlation of tumor necrosis factor alpha and interleukin 6 with hypertensive renal damage

Association between human metapneumovirus seroprevalence and hypertension in elderly subjects in a long-term care facility

Contact Info

Product

Resources

About

Effect of interleukin 8 and ICAM-1 on calcium-dependent outflow of K⁺ in erythrocytes from subjects with essential hypertension