Effect of low-power GaAlAs laser (660 nm) on bone structure and cell activity: an experimental animal study

Nicola, Renata A.; Jorgetti, Vanda; Rigau, Josepa; Pacheco, Marcos Tadeu Tavares; Reis, Luciene M. dos; Zângaro, Renato Amaro

doi:10.1007/s10103-003-0260-z

Cited by 144 publications

(117 citation statements)

References 32 publications

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“…A irradiação do laser de baixa potência tem sido utilizada terapeuticamente para cicatrização de lesões, tratamento de artrite reumatóide, dor musculoesquelética, entre outras aplicações clínicas. Efeitos benéficos são atribuídos à fotoestimulação dos tecidos, como melhora de cicatrização de lesões ulcerativas em indivíduos diabéticos e outros tipos de úlceras 18,19 , além de efeitos analgésicos 20 e antiinflamatórios 21 , estímulo à proliferação de células de diferentes origens 19,[22][23][24] e estimulação do reparo ósseo 18,24 . Os benefícios originados pela radiação laser de baixa potência são gerados pelos mecanismos não-térmicos da interação da luz com os tecidos, resultando em efeitos biomodulatórios por meio de interações fotoquímicas 8,24 .…”

Section: Introductionunclassified

Efeitos da aplicação do laser de baixa potência na regeneração do nervo isquiático de ratos

et al. 2010

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RESUMO: Os nervos periféricos sofrem constantes lesões de origem traumática, o que resulta em perdas funcionais. A terapia com laser de baixa potência vem sendo utilizada para minimizar os efeitos maléficos da inflamação e acelerar o processo de cicatrização dos tecidos lesados. Este estudo teve como objetivo verificar o efeito da irradiação do laser 830 nm no comportamento do nervo isquiático de ratos submetido a esmagamento. Foram utilizados 20 ratos, todos tendo tido o nervo isquiático esmagado, divididos em 4 grupos (n=5): P7 e P14, tratamento placebo por 7 e 14 dias; L7 e L14, tratamento por laser (dosagem de 4 J/cm²) por 7 e 14 dias. Os animais dos grupos P7 e P14 foram submetidos aos mesmos procedimentos, mas com o laser desligado. Os parâmetros analisados foram presença de infiltrado inflamatório e fibroblastos, destruição da bainha de mielina e degeneração axonal. Na análise estatística foi observada diferença estatística com relação a três parâmetros: os animais do grupo L14 apresentaram maior quantidade de fibroblastos (p=0,0001), menor degeneração da bainha de mielina (p=0,007) e menor quantidade de infiltrado inflamatório (p=0,001). A aplicação do laser de baixa potência contribuiu para a redução do processo inflamatório decorrente da lesão do nervo isquiático de ratos. DESCRITORES: Nervo ciático; Regeneração nervosa; Síndromes de compressão nervosa/reabilitação; Terapia a laser de baixa intensidade ABSTRACT: Peripheral nerves are commonly subject to traumatic injuries, leading to functional loss. Low-power laser therapy has been used in order to minimize harmful effects of inflammation and to accelerate healing of injured tissues. The purpose of this study was to assess the effect of 830 nm-laser irradiation on rat sciatic nerves submitted to crush. Twenty male Wistar rats had their sciatic nerve crushed and were divided into 4 groups (n=5): Sham7 and Sham14, placebo-treated for 7 and 14 days; L7 and L14, laser-treated (at 4 J/cm²) for 7 and 14 days. Sham group animals were submitted to the same procedures, but with the laser turned off. Assessed parameters were inflammatory infiltrates, fibroblasts, myelin sheath destruction, and axonal degeneration. The statistical analysis showed significant differences in three parameters: L14 animals showed more fibroblasts (p=0.0001), lesser myelin sheath degeneration (p=0.007), and lesser inflammatory infiltrates (p=0.001). Low-power laser therapy hence contributed to reduce the inflammatory process due to rat sciatic nerve injury.

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Section: Introductionunclassified

Efeitos da aplicação do laser de baixa potência na regeneração do nervo isquiático de ratos

et al. 2010

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“…It is considered a noninvasive, safe technique to stimulate osteogenesis [23,24]. Several previous studies have shown that LLLT could increase both osteoblast and osteoclast activities, together with stimulating the formation of callus and new bone matrix [25][26][27][28]. Of note, LLLT alone as a treatment modality in surgical defects enhanced faster and a greater area of bone formation compared to the PRF treatment modality.…”

Section: Discussionmentioning

confidence: 99%

Usage of Low Level Laser Biostimulation and Platelet Rich Fibrin in Bone Healing: Experimental Study

Elhayes¹,

Zaky²,

Ibrahim³

et al. 2016

Dent. Med. Probl.

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Low level laser therapy (LLLT) is defined as supplying direct biostimulative light energy to the cells. The wound healing process could be enhanced using low-level semiconductor diode lasers [1]. It has been reported that absorbed laser energy stimulates the molecules and atoms of the body's cells [2].While several studies have demonstrated that LLLT has stimulating effects on stem cells of the AbstractBackground. Experimental studies have shown that low level laser therapy (LLLT) has a positive local biostimulative effect in the early stage of bone healing. Platelet rich fibrin (PRF) also has been shown to be effective in the treatment of intrabony periodontal defects. Objectives. The objective of our experimental study was to demonstrate the combined effects of LLLT and PRF on bone healing. Material and Methods. Our experimental study was done over 80 bony cavities in 20 adult male rabbits, aged 12 months. An incision was made for exposure of the femur bone of all rabbits. Then, by using a large, round surgical bur, a perforated hole was made in the femur. The cavities induced in these rabbits were divided into 4 groups: The control group which was neither subjected to any laser irradiation nor filled with any bone substitute (group I); The bony defects were filled with PRF (group II); The cavities were subjected to low level laser (LLL) for biostimulation (group III); The cavities were subjected to LLL for biostimulation then were filled with PRF (group IV). Histological assessments of the four groups were done using a hematoxylin and eosin stain. Statistical analysis was done using ANOVA and Bonferroni tests for comparisons between the four groups.Results. The area percentage of the newly formed bone in group IV was significantly higher than the other three groups. The area percentage of the newly formed bone in group III is significantly higher than group II. Conclusions. LLLT could induce bone formation in the bone defect at a faster rate than PRF. However, a combination of both LLLT and PRF as treatment modalities could induce bone formation in the bone defect more than that of LLLT or PRF alone (Dent. Med. Probl. 2016, 53, 3, 338-344).

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“…Garcia et al [24] demonstrated that the LLLT was effective for stimulating bone formation in critical sized defects in the calvaria of rats submitted to ovariectomy. Nicola et al [25] observed that applying LLLT on injured bones increased the bone volume, osteoblasts surface, and mineral apposition rates, suggesting that LLLT increased. Our results confirm the positive bio-stimulatory effect of LLLT, which depends mainly on the ability of the tissue to respond to light energy.…”

Section: Discussionmentioning

confidence: 99%

Effect of Diode Laser on Healing of Tooth Extraction Socket: An Experimental Study in Rabbits

Hamad

Naif

Abdullah

2015

J. Maxillofac. Oral Surg.

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Objectives To evaluate the effect of low-level laser therapy on healing of extracted tooth socket of healthy rabbits. Design The sample of this study was 20 male rabbits of 2-2.5 kg weight with age range of 8-12 months. Right and left lower first premolar teeth were extracted. The extraction sockets of lower right first premolar were irradiated with 0.9 W gallium-aluminum-arsenide (GaAlAs) diode laser for 5 min, immediately after extraction and then every 72 h for the next 12 days. The extraction socket of left side were not exposed to laser and served as a control. The animals were sacrificed after 7, 14, 30 and 45 days and the experimental and control sockets were removed from the harvested mandibles and prepared for haematoxylin and eosin staining and Masson's stain. The prepared slides were examined under light microscope for histological and histomorphometric examination. Results The histological examination showed that diode laser-treated sockets demonstrated early formed new bone with faster maturation of primary bone to secondary bone as compared to non-treated control sockets. Histomorphometric analysis revealed a statistically significant increase in the density and volume of trabecular bone in lasertreated sockets than control sockets. Conclusion Diode laser application to tooth extraction socket has a positive effect on bone formation.

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Effect of low-power GaAlAs laser (660 nm) on bone structure and cell activity: an experimental animal study

Cited by 144 publications

References 32 publications

Efeitos da aplicação do laser de baixa potência na regeneração do nervo isquiático de ratos

Efeitos da aplicação do laser de baixa potência na regeneração do nervo isquiático de ratos

Usage of Low Level Laser Biostimulation and Platelet Rich Fibrin in Bone Healing: Experimental Study

Effect of Diode Laser on Healing of Tooth Extraction Socket: An Experimental Study in Rabbits

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