Effect of oxygen plasma etching on pore size-controlled 3D polycaprolactone scaffolds for enhancing the early new bone formation in rabbit calvaria

Kook, Min-Suk; Roh, Hee-Sang; Kim, Byung–Hoon

doi:10.4012/dmj.2017-318

Cited by 15 publications

(10 citation statements)

References 58 publications

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“…As hydrophilicity is an indicator of histocompatibility, the enhanced hydrophilicity in this study demonstrated corresponding enhancement in tissue regeneration ability, cell increment, as well as cell adhesion of the obtained scaffolds. 21–23 This result suggests that PLA/CA fibers may be more conducive to tissue growth than PLA fibers.…”

Section: Discussionmentioning

confidence: 91%

Emulsion electrospun PLA/calcium alginate nanofibers for periodontal tissue engineering

Shen

et al. 2019

J Biomater Appl

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The regeneration of periodontal bone tissue is a major obstacle in tissue engineering. We have recently designed a compound nanofiber scaffold for the tissue repair of periodontal bone, which was composed of poly(lactic acid) (PLA) and calcium alginate (CA). The obtained improvements in both physical properties and biological effects on periodontal tissue were systematically evaluated and discussed. Method: Neat PLA and PLA/CA nanofiber scaffolds were prepared by electrospinning technology. Scanning electron microscopy was applied for the observation of surface morphology. Their mechanical properties were evaluated in terms of elongation at break and elastic modulus. Hydrophilic/hydrophobic nature of the nanofibers was investigated by a stereomicroscope, and their vitro degradation was tested as well. Cell proliferation and adhesion were detected in order to evaluate the biocompatibility of nanofiber scaffolds. Expression of cell mineralization gene and formation of mineralization knots were examined so as to assess scaffolds' capability of regenerating bone tissues. The expression of Toll-like receptor 4 (TLR4), IL-6, IL-8, and IL-1b was utilized to decide the possibility of tissue inflammation caused by the nanofibers with the assistance of reverse transcription-polymerase chain reaction (RT-PCR) assay. Results: The obtained nanofibers possessed uniform surface morphology. The elongation at break and elastic modulus of PLA/CA nanofiber scaffolds were higher than those of neat PLA. CA could enhance the hydrophilicity of PLA fibers; in the meantime, it promoted cell adhesion of periodontal ligament cells (PDLCs) and bone marrow stromal cells (BMSCs). Addition of CA had raised the expression level of cell mineralization gene and the formation of mineralization knots (BMSCs). On the other hand, both neat PLA fibers and PLA/CA fibers could induce higher expression level of inflammatory mediators and TLR4 of human periodontal ligament cells (hPDLCs). Conclusions: The introduction of CA enhanced the mechanical properties of neat PLA nanofibers and increased its hydrophilicity. Neat PLA nanofibers and PLA/CA scaffold could both facilitate BMSCs proliferation, while the addition of CA promoted BMSCs osteogenic differentiation. The improved (or enhanced) expression of inflammatory mediators caused by nanofibers might be regulated via TLR4 pathway.

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Section: Discussionmentioning

confidence: 91%

Emulsion electrospun PLA/calcium alginate nanofibers for periodontal tissue engineering

Shen

et al. 2019

J Biomater Appl

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“…One of the advantages of 3D printing is its ability to control the scaffold architecture’s internal structure. In present work, optimal design of 3D scaffold architecture’s internal structure was determined through the results of our previous studies [ 30 , 31 ]. For example, a 3D scaffold used in this work fabricated a 0°/45° strut layout pattern and 300 μm pore size.…”

Section: Discussionmentioning

confidence: 99%

Amine Plasma-Polymerization of 3D Polycaprolactone/β-Tricalcium Phosphate Scaffold to Improving Osteogenic Differentiation In Vitro

Kim

2022

Materials

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This study aims to investigate the surface characterization and pre-osteoblast biological behaviors on the three-dimensional (3D) poly(ε-caprolactone)/β-tricalcium phosphate (β-TCP) scaffold modified by amine plasma-polymerization. The 3D PCL scaffolds were fabricated using fused deposition modeling (FDM) 3D printing. To improve the pre-osteoblast bioactivity, the 3D PCL scaffold was modified by adding β-TCP nanoparticles, and then scaffold surfaces were modified by amine plasma-polymerization using monomer allylamine (AA) and 1,2-diaminocyclohexane (DACH). After the plasma-polymerization of PCL/β-TCP, surface characterizations such as contact angle, AFM, XRD, and FTIR were evaluated. In addition, mechanical strength was measured by UTM. The pre-osteoblast bioactivities were evaluated by focal adhesion and cell proliferation. Osteogenic differentiation was investigated by ALP activity, Alizarin red staining, and Western blot. Plasma-polymerization induced the increase in hydrophilicity of the surface of the 3D PCL/β-TCP scaffold due to the deposition of amine polymeric thin film on the scaffold surface. Focal adhesion and proliferation of pre-osteoblast improved, and osteogenic differentiation was increased. These results indicated that 3D PCL/β-TCP scaffolds treated with DACH plasma-polymerization showed the highest bioactivity compared to the other samples. We suggest that 3D PCL/β-TCP scaffolds treated with DACH and AA plasma-polymerization can be used as a promising candidate for osteoblast differentiation of pre-osteoblast.

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“…It was found that rough and smooth surfaces induce different kinds of cell responses; for example, osteoblast cells prefer rougher surface, periodontal fibroblast cells attach better to smoother surfaces, and epithelial cells adhere to the smooth surfaces . Various surface treatment techniques such as plasma treatment, grinding, chemical and mechanical etching, grit blasting, 3D printing, lithography, micromachining, acid etching, ion etching, and electrospinning have been developed to create and improve roughness on the surface of biomaterials. There are two categories of materials tested for studying the impact of roughness on cellular functions.…”

Section: Effects Of Surface Properties On Cellular Behaviorsmentioning

confidence: 99%

Controlling Cell Behavior through the Design of Biomaterial Surfaces: A Focus on Surface Modification Techniques

Amani

Arzaghi

Bayandori

et al. 2019

Adv Materials Inter

321

200

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Surface interaction at the biomaterial–cell interface is essential for a variety of cellular functions, such as adhesion, proliferation, and differentiation. Nevertheless, changes in the biointerface enable to trigger specific cell signaling and result in different cellular responses. In order to manufacture biomaterials with higher functionality, biomaterials containing immobilized bioactive ligands have been widely introduced and employed for tissue engineering and regenerative medicine applications. Moreover, a number of physical and chemical strategies have been used to improve the functionality of biomaterials and specifically at the material interface. Here, the interactions between materials and cells at the interface levels are described. Then, the importance of surface properties in cell function is discussed and recent methods for surface modifications are systematically highlighted. Additionally, the impact of bulk material properties on the cellular responses is briefly reviewed.

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Effect of oxygen plasma etching on pore size-controlled 3D polycaprolactone scaffolds for enhancing the early new bone formation in rabbit calvaria

Cited by 15 publications

References 58 publications

Emulsion electrospun PLA/calcium alginate nanofibers for periodontal tissue engineering

Emulsion electrospun PLA/calcium alginate nanofibers for periodontal tissue engineering

Amine Plasma-Polymerization of 3D Polycaprolactone/β-Tricalcium Phosphate Scaffold to Improving Osteogenic Differentiation In Vitro

Controlling Cell Behavior through the Design of Biomaterial Surfaces: A Focus on Surface Modification Techniques

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