Effect of Probiotics and Triple Eradication Therapy on the Cyclooxygenase (COX)‐2 Expression, Apoptosis, and Functional Gastric Mucosal Impairment in <i> Helicobacter pylori</i>‐Infected Mongolian Gerbils

Brzozowski, Tomasz; Konturek, Peter C.; Mierzwa, Marzena; Drozdowicz, Danuta; Bielański, Władysław; Kwiecień, Sławomir; Konturek, Stanisław J.; Stachura, Jerzy; Pawlik, Wiesław W.; Hahn, Eckhart G.

doi:10.1111/j.0083-8703.2006.00373.x

Cited by 43 publications

(21 citation statements)

References 48 publications

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“…Numerous studies have shown that apoptotic cell death of gastric mucosal cells has an important role in GML (18)(19)(20). In line with this, the present study found that the expression of Bax, caspase-3 and caspase-7 was higher in the stomach tissues of HAPC-induced GML patients than that in the healthy controls, suggesting that cell apoptosis also takes an active part in HAPC-induced GML.…”

Section: Discussionsupporting

confidence: 90%

Mechanism of apoptosis involved in gastric mucosal lesions in Tibetans with high-altitude polycythemia

Li¹,

Gesang²,

Cai³

2017

Experimental and Therapeutic Medicine

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Section: Discussionsupporting

confidence: 90%

Mechanism of apoptosis involved in gastric mucosal lesions in Tibetans with high-altitude polycythemia

Li¹,

Gesang²,

Cai³

2017

Experimental and Therapeutic Medicine

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“…These include interleukin 8 and tumour necrosis factor α, which have been reported to increase gastrin expression. H pylori also activates gastrin releasing peptide (through the type IV secretion system) and regulates the gastrin modulator somatostatin to increase gastrin expression 38 39…”

Section: Discussionmentioning

confidence: 99%

Helicobacter pylori potentiates epithelial:mesenchymal transition in gastric cancer: links to soluble HB-EGF, gastrin and matrix metalloproteinase-7

Yin

Grabowska

Clarke

et al. 2010

Gut

106

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Background and aimsHelicobacter pylori (H pylori) infection is a major risk factor in the development of distal gastric adenocarcinoma. Development of the invasive phenotype is associated with the phenomenon of epithelial:mesenchymal transition (EMT). Soluble heparin-binding epidermal growth factor (HB-EGF) has been implicated in this process. A study was undertaken to investigate the possibility that matrix metalloproteinase (MMP)-7 is upregulated in H pylori infection as a result of hypergastrinaemia, which may enhance shedding of HB-EGF and contribute towards EMT in gastric adenocarcinoma cell lines.MethodsThree gastric epithelial cell lines (AGS, MGLVA1 and ST16) were co-cultured with the pathogenic H pylori strain 60190 and non-pathogenic strain Tx30a in an in vitro infection model. Gene expression was quantified by real-time PCR, HB-EGF shedding by ELISA and protein expression by immunofluorescence or immunohistochemistry. The INS-GAS mouse, a transgenic mouse model of gastric carcinogenesis which overexpresses amidated gastrin, was used to investigate the in vivo relationship between HB-EGF, MMP-7, gastrin and EMT.ResultsThe pathogenic strain of H pylori significantly upregulated EMT-associated genes Snail, Slug and vimentin in all three gastric cell lines to a greater degree than the non-pathogenic strain. Pathogenic H pylori also upregulated HB-EGF shedding, a factor implicated in EMT, which was partially dependent on both gastrin and MMP-7 expression. Gastrin and MMP-7 siRNAs and MMP-7 neutralising antibody significantly reduced upregulation of HB-EGF shedding in H pylori infected gastric cell lines and reduced EMT gene expression. The effect of H pylori on EMT was also reversed by gastrin siRNA. Neutralisation of gastrin in the INS-GAS mouse model reduced expression of MMP-7, HB-EGF and key EMT proteins.ConclusionThe upregulation of MMP-7 by pathogenic H pylori is partially dependent on gastrin and may have a role in the development of gastric cancer, potentially through EMT, by indirectly increasing levels of soluble HB-EGF.

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“…COX-2 was upregulated in atherosclerotic lesions [101] and catalyzes the production of the majority of vascular prostanoids in human atherosclerotic areas [102]. Down-regulated COX-2 was found in helicobacter pylori-infected mongolian gerbils with a commercial probiotic Lacidofil treatment [103]. Patel and colleagues demonstrated that Lactobacillus acidophilus pretreatment decreased COX-2 expression in catla thymus macrophages compared to Aeromonas hydrophila and co-stimulated macrophages [104].…”

Section: Modes Of Action Of Probiotic Bacteria In Antioxidationmentioning

confidence: 99%

Antioxidant Properties of Probiotic Bacteria

et al. 2017

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Oxidative stress defines a condition in which the prooxidant–antioxidant balance in the cell is disturbed, resulting in DNA hydroxylation, protein denaturation, lipid peroxidation, and apoptosis, ultimately compromising cells’ viability. Probiotics have been known for many beneficial health effects, and the consumption of probiotics alone or in food shows that strain-specific probiotics can present antioxidant activity and reduce damages caused by oxidation. However, the oxidation-resistant ability of probiotics, especially the underling mechanisms, is not properly understood. In this view, there is interest to figure out the antioxidant property of probiotics and summarize the mode of action of probiotic bacteria in antioxidation. Therefore, in the present paper, the antioxidant mechanisms of probiotics have been reviewed in terms of their ability to improve the antioxidant system and their ability to decrease radical generation. Since in recent years, oxidative stress has been associated with an altered gut microbiota, the effects of probiotics on intestinal flora composition are also elaborated.

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Effect of Probiotics and Triple Eradication Therapy on the Cyclooxygenase (COX)‐2 Expression, Apoptosis, and Functional Gastric Mucosal Impairment in Helicobacter pylori‐Infected Mongolian Gerbils

Cited by 43 publications

References 48 publications

Mechanism of apoptosis involved in gastric mucosal lesions in Tibetans with high-altitude polycythemia

Mechanism of apoptosis involved in gastric mucosal lesions in Tibetans with high-altitude polycythemia

Helicobacter pylori potentiates epithelial:mesenchymal transition in gastric cancer: links to soluble HB-EGF, gastrin and matrix metalloproteinase-7

Antioxidant Properties of Probiotic Bacteria

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