Effect of quercetin and its metabolites isorhamnetin and quercetin-3-glucuronide on inflammatory gene expression: role of miR-155

Boesch-Saadatmandi, Christine; Łoboda, Agnieszka; Wagner, Anika E.; Stachurska, Anna; Józkowicz, Alicja; Dulak, Józef; Döring, Frank; Wolffram, Siegfried; Rimbach, Gerald

doi:10.1016/j.jnutbio.2010.02.008

Cited by 224 publications

(186 citation statements)

References 47 publications

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“…This phenomenon may be controlled by the transcription factor NF-κB. Many reports have mentioned that quercetin exerts its modulatory effects by reduction of pro-inflammatory genes through NF-κB inactivation (Boesch-Saadatmandi et al, 2011;Cho et al, 2003;Nair et al, 2006). From previous studies, quercetin inhibits the production of many proinflammatory cytokines, including IL-1β and TNF, the findings from the present work suggest that quercetin may be able to modulate the immune response via the NF-κB pathway.…”

Section: Discussionsupporting

confidence: 59%

“…Even though the two experiment settings used quercetin at 50 µM final concentration, the course of inflammatory processes might originate from diverse pathways. In fact, both bacterial cells (in the current study) and cell wall component (LPS) have one thing in common to initiate inflammatory signals, nevertheless, quercetin could possibly lessen proinflammatory gene expressions via inhibition of key transcription factors (Boesch-Saadatmandi et al, 2011). These phenomena could emphasize the important property of quercetin in specifically targeted of transcription factors.…”

Section: Discussionmentioning

confidence: 68%

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Quercetin Promotes the Expression of Genes Involved in Phagocytosis in Bovine Neutrophils

Srikok

Nambut

Wongsawan

et al. 2017

American Journal of Animal and Veterinary Sciences

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This study was conducted to explore the effect of Quercetin (QH) on phagocytosis of bovine neutrophils. Neutrophils were isolated from fifteen multiparous Holstein cows. We in vitro treated neutrophils with PBS or 50 µM of Quercetin (QH) or Cytochalasin B (CytB), as phagocytosis inhibitor, prior to monitoring phagocytosis of Escherichia coli by flow cytometry and microscopic examination. Additionally, the expressions of CORO1A, CYBA (gp91 phox ), LAMP1, RAB7A, RAC1 and PAK1 mRNA were analyzed by real-time PCR. In the time-course experiment, treated neutrophils were allowed to co-cultured with live bacteria for 30, 60 and 90 min before measuring gene expressions. The expression levels of IL-1 β and TNF genes in order to demonstrate the antiinflammatory property of quercetin were assessed by conventional RT-PCR. The results of flow cytometry and microscopic examination showed that the percentage of neutrophils performing phagocytosis were significantly higher in QH group in comparison with other groups. We reported here that mRNA expressions of CORO1A, CYBA, LAMP1, RAB7A, RAC1 and PAK1 genes involved in phagocytosis, were significantly up-regulated in QH group. As expected, CytB group had profound down-regulation of genes with one exception in PAK1. From our observations, the expression levels of phagocytic process genes in the QH group were optimum at 60 min and started to decline at 90 min of incubation. The data also indicated that quercetin inhibited inflammation by reducing the expressions of both IL-1β and TNF. In conclusion, the information in our experiments conclude that quercetin has the ability to boost the expression of genes involved in phagocytosis while reduces the expression of proinflammatory genes.

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Section: Discussionsupporting

confidence: 59%

Section: Discussionmentioning

confidence: 68%

Quercetin Promotes the Expression of Genes Involved in Phagocytosis in Bovine Neutrophils

Srikok

Nambut

Wongsawan

et al. 2017

American Journal of Animal and Veterinary Sciences

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“…Ramyaa et al 50 have already reported the modulatory effect of quercetin on oxidative stress by upregulating Nrf-2 expression and downregulating NFkB and COX-2 expression. The anti-inflammatory properties of quercetin accompanied by an increase in HO-1 protein levels associated with elevated nuclear translocation of Nrf-2 were previously exhibited by Boesch-Saadatmandi et al 51 in murine RAW 264.7 macrophages. On the other hand, quercetin acts as an anti-inflammatory agent in lipopolysaccharide-induced acute lung injury by significantly reducing COX-2 and iNOS expression, and NFkB p65 phosphorylation.…”

Section: Discussionsupporting

confidence: 53%

Effect of the Lipid Peroxidation Product 4-Hydroxynonenal on Neuroinflammation in Microglial Cells: Protective Role of Quercetin and Monochloropivaloylquercetin

Cumaoğlu

Agkaya

Ozkul

2019

tjps

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Objectives:The lipid peroxidation-derived aldehyde 4-hydroxynonenal (HNE) has been implicated in a number of oxidative stress-induced inflammatory pathologies such as neurodegenerative diseases and aging. In this regard, we investigated the effects of HNE on neuroinflammatory responses by measuring cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS) induction with cytokine production. In addition, we measured nuclear factor erythroid 2-related factor 2 (Nrf-2)/Kelch-like ECH-associated protein 1 (Keap1) signaling proteins, and antioxidant enzymes heme oxygenase-1 (HO-1) and nicotinamide adenine dinucleotide phosphate dehydrogenase, quinone 1 (NQO1), and compared the results with quercetin and monochloropivaloylquercetin (MPQ) pretreated microglial cells. Materials and Methods: Cytotoxicity was determined by MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay and production of cytokines was determined by cytokine array. Furthermore, intracellular Nfr2/Keap1 signaling proteins, HO-1, NQO1, and COX-2 expression were analyzed by western blot in 2.5 μM HNE treated BV-2 cells. Results: Inducible nitric oxide synthase (iNOS) and COX-2 mRNA levels were measured with reverse transcription-quantitative polymerase chain reaction. HNE induced both COX-2 mRNA and protein levels, iNOS mRNA expression, and cytokine production. In addition, HNE markedly increased Keap1 levels and decreased cytoplasmic Nrf-2 expression with antioxidant enzyme HO-1 levels. Quercetin and monochloropivaloylquercetin treatment alleviated neuroinflammatory responses in microglial cells, by decreasing COX-2 mRNA expression. Monochloropivaloylquercetin decreased cytoplasmic Keap1 levels and increased nuclear translocation of Nrf-2 resulted in induction of HO-1 and NQO1 expression. Conclusion: These results suggest that HNE could be a link between oxidative stress and inflammation in BV-2 microglia cells. In particular, monochloropivaloylquercetin alleviated inflammation, probably by decreasing the expression of proinflammatory genes and strengthening the antioxidant defense system. Key words: 4-Hydroxynonenal, microglia, quercetin, inflammation, cyclooxygenase-2, hemeoxygenase-1 ÖZ Amaç: Lipit peroksidasyonu ürünü aldehit, 4-hidroinonenal (HNE), nörodejeneratif hastalıklar ve yaşlanma gibi oksidatif strese bağlı inflamatuvar patolojilerle ilişkilendirilmiştir. Bu bağlamda, HNE'nin nöroinflamatuvar cevap üzerine etkisini siklooksijenaz-2 (COX-2), indüklenebilir nitrik oksit sentaz (iNOS) ifadelenmeleri ve sitokin üretimi üzerinden incelenmiştir. Bunlara ek olarak, nükleer faktör eritroid 2-ilişkili faktör 2 (Nrf2)/ Kelch-benzeri ECH-ilişkili protein 1 (Keap1) sinyal proteinleri, antioksidan enzimler hemoksijenaz-1 (HO-1) ve nikotinamid adenin dinükleotit fosfat dehidrojenaz, kinon 1 (NQO1) ifadelenme düzeyleri ölçüldü ve sonuçlar kersetin ve monokloropivaloilkersetin uygulanan hücrelerle karşılaştırılmıştır. Gereç ve Yöntemler: Sitotoksisite MTT (3-(4,5-dimetiltiyazol-2-yl)-2,5-difeniltetrazolyum bromit) indirgenme y...

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“…Antioxidant activity [4], [5], [6], [7], [8] [16], [17], [18], [19], [20], [21], [22] Anticancer activity [2], [23], [24], [25], [26] Cardiovascular protection [4], [27], [28], [29], [30] Antimicrobial activity [31], [32], [33] Anti-inflammatory activity [34], [35], [36], [37], [38], [39] Hepatoprotective activity [40], [41], [42] …”

Section: Pharmacological Property Referencesmentioning

confidence: 99%

“…Furthermore, quercetin applied on a cultured hepatocyte cell line has been reported to show an inhibitory effect against inflammatory causing agents like reactive C-protein and nitric oxide synthase [36]. However, in vivo study in mice has been reported to show a reduction in expression of inflammatory genes by using quercetin enriched diet [37]. An improvement in insulin sensitivity has been reported by intraperitoneal administration of quercetin in mice, subsequently a reduction in inflammation was reported which is attributed to the insulin resistance [38].…”

Section: Antiinflammatory Activitymentioning

confidence: 99%

Pharmacological Applications of Quercetin and its Derivatives: A Short Review

Maalik¹,

Fa²,

Mumtaz³

et al. 2014

Trop. J. Pharm Res

125

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Quercetin (3,3ʹ,4ʹ,5,7-pentahydroxyl- flavone) is a flavonol, and it belongs to a class of plant secondary metabolites known as flavonoids. It is present in man's daily diet and is known for biological activities such as antioxidant, antiviral, anticancer, antimicrobial, anti-inflammatory and many more. Quercetin has been reported for its antioxidant and antiviral applications, hence, it is not only used as such but also its various derivatized forms have potentials for development into drugs for the treatment of diseases caused by oxidative stress and lethal viruses.

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Effect of quercetin and its metabolites isorhamnetin and quercetin-3-glucuronide on inflammatory gene expression: role of miR-155

Cited by 224 publications

References 47 publications

Quercetin Promotes the Expression of Genes Involved in Phagocytosis in Bovine Neutrophils

Quercetin Promotes the Expression of Genes Involved in Phagocytosis in Bovine Neutrophils

Effect of the Lipid Peroxidation Product 4-Hydroxynonenal on Neuroinflammation in Microglial Cells: Protective Role of Quercetin and Monochloropivaloylquercetin

Pharmacological Applications of Quercetin and its Derivatives: A Short Review

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