Effect of trypsin, phospholipases, and membrane-impermeable reagents on the uptake of palmitic acid by isolated rat liver cells

Physicochemical and immunohistological studies of a sulfobromophthalein- and bilirubin-binding protein from rat liver plasma membranes.

“…carrier-mediated transport system (1)(2)(3)(4)(5), and that, despite some overlap (5-7), a separate system may be primarily responsible for bile acid uptake (1,8,9). The hepatocellular uptake mechanism for fatty acids is highly controversial, but at least some studies suggest that these, too, are sequestered by a saturable membrane-associated mechanism (46). Since the first step in any membrane-associated carrier-mediated transport process involves binding to the carrier, several studies have focused on the binding of these anionic ligands to LPM, and attempts to identify and isolate putative receptors.…”

Uptake of oleate by isolated rat adipocytes is mediated by a 40-kDa plasma membrane fatty acid binding protein closely related to that in liver and gut.

“…3 (21). DISCUSSION Although it has long been considered that the movement of long-chain fatty acids across cell membranes is invariably passive (1)(2)(3)(4), previous studies had already suggested that fatty acids might be taken up in at least some cell types by one or more saturable membrane-associated processes (17,18,(32)(33)(34)(35). Unfortunately, none of these earlier studies was conclusive.…”

Antibacterial and Cytotoxic Aspects of Longchain Fatty Acids as Cell Surface Events: Selected Topics

“…The evidence was obtained from the physicochemical changes of fatty acid-treated proteins as revealed by polyacrylamide gel electrophoresis (Sheu and Freese, 1972;Fessenden-Raden, 1972). Mahadevan and Sauer (1974) also felt a possibility that proteins (lipoproteins) buried below the surface layer of plasma membrane can play a role in the uptake of fatty acids.…”