Effect of tubal explants and their secretions on bovine spermatozoa: modulation of ROS production and DNA damage

Gómez, Patricia Navarrete; Álvarez, Juan G.; Risopatrón, Jennie; Romero, Fernando; Sánchez, Raúl

doi:10.1071/rd11180

Cited by 5 publications

(3 citation statements)

References 48 publications

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“…In cows, GPx3 [ 52 ] and GPx4 [ 53 ] were under-expressed in the ampulla and over-expressed in the isthmus. This is because the ampulla is the site of fertilization, and spermatozoa need ROS presence for the capacitation to occur, while spermatozoa in the isthmus need to be protected against ROS-induced damage as they constitute a sperm reservoir [ 54 ]. High GPxs activity was also detected in cow oviductal fluid.…”

Section: Discussionmentioning

confidence: 99%

GPx8 Expression in Rat Oocytes, Embryos, and Female Genital Organs During Preimplantation Period of Pregnancy

Mihálik

Kreheľová

Kovaříková

et al. 2020

IJMS

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This study aimed to detect the presence of glutathione peroxidase 8 (GPx8) in rat during preimplantation period of pregnancy. Females were killed on first (D1), third (D3), and fifth (D5) day of pregnancy. The presence of GPx8 in embryos was detected under the confocal microscope, the presence of GPx8 in genital organs was confirmed immunohistochemically, and the amount of GPx8 was determined using densitometry. We found that GPx8 is dispersed in the cytoplasm of oocytes, while after fertilization, it is concentrated in granules. From 4-cell stage till blastocyst, GPx8 reaction was found in the perinuclear region. In the ovary, GPx8 was seen in granulosa-lutein cells, in plasma of blood vessels, and inside Graafian follicles. In oviduct, GPx8 was detected in the plasma and in the extracellular matrix (ECM). Moreover, epithelial cells of isthmus were positive. In uterus, GPx8 was observed in the uterine glands, in the plasma, and in ECM. On D5, the enzyme disappeared from the uterine glands and appeared in fibroblasts. Densitometry revealed that the highest amount of GPx8 was on D1 and subsequently declined. To our knowledge, this is the first paper describing GPx8 presence in the oocytes, preimplantation embryos, and female genital organs in mammals. Our results improve the understanding of antioxidant enzymes presence during pregnancy in defense against oxidative stress, which is considered to be one of the main causes of infertility.

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Section: Discussionmentioning

confidence: 99%

GPx8 Expression in Rat Oocytes, Embryos, and Female Genital Organs During Preimplantation Period of Pregnancy

Mihálik

Kreheľová

Kovaříková

et al. 2020

IJMS

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“…In addition, antioxidant activity in the oviduct environment has been correlated to spermatic and oocyte protection against ROS damage in the female reproductive tract (Lapointe & Bilodeau, ). According to Gómez, Alvarez, Risopatrón, Romero, and Sánchez (), low levels of ROS play a key role in sperm function; however, an increase in ROS generation above these levels may result in the induction of sperm membrane and DNA damage. Georgiou et al () identified secretory proteins from the oviduct, such as SOD, glutathione reductase and thioredoxin families (all identified in the protein network analysis in this study) as the main components of mammalian enzymatic redox pathways that control extracellular ROS levels.…”

Section: Discussionmentioning

confidence: 99%

Proteome of the periovulatory oviduct and uterus of goats as related to nutritional balance

Fernandes

Rodríguez-Villamil

Vasconcelos

et al. 2018

Reprod Domestic Animals

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This study was conducted to evaluate the effects of different feeding levels on the proteome of oviduct and uterus tissues of hormonally stimulated goats during the periovulatory period. Forty goats were separated into four different diet groups: Diet 1.0 M (n = 11), Diet 1.3 M (n = 10), Diet 1.6 M (n = 9), Diet 1.9 M (n = 10), fed with 1.0, 1.3, 1.6 and 1.9 times live weight maintenance, respectively. After four weeks of treatment, six hormonally stimulated females per treatment group were randomly selected for collection of uterine and the oviduct tissue samples. Samples were collected after animals were slaughtered in a commercial unit. Feeding goats with 1.3 to 1.9 times more nutrients than a control group directly influenced the proteome of the oviduct and uterus, altering the expression of proteins that participate in biological processes such as apoptosis, antioxidant, and immunological activities. These events are crucial for fertilization and early embryonic survival. Expression of oviduct proteins such as Tubulin Beta 2B, Transferrin and Disulphide-isomerase A3 increased in the 1.9 M group in relation to the other feeding levels. Disulphide-isomerase A4 showed higher expression in the 1.0 M group compared to diets with higher energetic levels. As energy intake increased in the diets, there was higher expression of Alpha-1-antitrypsin and downregulation of Profilin-1 in the uterus of the goats. In conclusion, this study showed that specific proteins of the goat oviduct and uterus expressed during the periovulatory period are modified as the result of nutritional balance.

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“…On the other hand, a recent study by Gómez et al. () implies that significant oxidative stress may take place in the oviduct, particularly during short‐term incubation, and this may be related to changes in the antioxidant factors present in the oviducal cells and associated secretions.…”

Section: Fluorescent Detection Of Sperm Function – ‘Known Knowns Unkmentioning

confidence: 99%

Fluorescence Technologies for Evaluating Male Gamete (Dys)Function

Petrunkina

Harrison²

2013

Reprod Domestic Animals

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ContentsFor many years, andrologists have sought ways of assessing sperm fertility, especially of new sires entering the breeding chain. As knowledge of the complex processes that enable sperm to fertilize eggs has increased, it has become clearer that quantitative estimation of the fertilizing potential of a sire or an ejaculate is actually unlikely ever to be fully realized. Here, we propose that a better approach is to identify substandard males and semen samples. During the past decades, the use of fluorescence technologies in biomedical science has burgeoned, with the development of very powerful instrumentation such as confocal microscopy and flow cytometers of ever-increasing capabilities together with a vast range of fluorochromes and fluorochrome conjugates. This technology has been applied to andrology but thus far in only a relatively simple way. In this review, we offer strategies for assessing a large range of sperm functions thought to be related to fertilizing ability over a temporal window rather than at a single time point. From such an assessment profile, sperm samples that overrespond or do not respond sufficiently could be identified, termed dysfunctional and rejected. We outline the rationales behind such tests, present information on new potentially useful fluorochromes and current flow cytometer models that would be suitable for the multicolour multifunctional tests we propose, and we offer suggestions as to how andrologists might design such multicolour tests for themselves.

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Effect of tubal explants and their secretions on bovine spermatozoa: modulation of ROS production and DNA damage

Cited by 5 publications

References 48 publications

GPx8 Expression in Rat Oocytes, Embryos, and Female Genital Organs During Preimplantation Period of Pregnancy

GPx8 Expression in Rat Oocytes, Embryos, and Female Genital Organs During Preimplantation Period of Pregnancy

Proteome of the periovulatory oviduct and uterus of goats as related to nutritional balance

Fluorescence Technologies for Evaluating Male Gamete (Dys)Function

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