Effect of UVA Light on the Activity of Several Aged Human Lens Enzymes

Linetsky, Mikhail; Chemoganskiy, Vitaliy; Hu, Fang; Ortwerth, B.J.

doi:10.1167/iovs.02-0597

Cited by 23 publications

(21 citation statements)

References 62 publications

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“…Sebanyak 50 µL hasil larutan homogenate lensa ditambahkan kedalam 1,55mL campuran larutan yang terdiri dari 100 mM buffer KPO4 (pH 8,0), 1,0 mM EDTA, 1,0 mM glutation teroksidasi (GSSG) dan 0,2 mM NADPH. dan penurunan densitas optical dari larutan ini dicatat dengan menggunakan spektrofotometer ( Spektrofotometer fluorescen merk Simatsu 2000 ) pada panjang gelombang 340 nm yang direkam pada suhu 25° C. Satu unit enzim didefinisikan sebagai 1 µmol/min NADPH teroksidasi pada 25° C (4,12).…”

Section: -Pebruari 2006unclassified

Hubungan Antara Kadar Enzim Glutation Reduktase Dengan Derajat Kekeruhan Inti Lensa

Sulistya

Dewi

Suyuti

et al. 2013

JKB

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The aim of this study was to determine the correlation between the level of glutathione reductase [GR] in relation with the severity of nuclear cataract and the increase of age. An analytic cross sectional observasional study of nuclear cataract patient seen at Saiful Anwar Hospital and Mass Cataract Surgery performed by Perdami Malang was carried out. Patients were classified into 3 groups based on age and 4 groups based on severity of nuclear cataract. The samples were taken by consecutive sampling and were operated on visual indication. The extracted lens were examined for [GR]. The laboratory findings of [GR] were compared with the severity of senile cataract. The data were analyzed with one way ANOVA. Confidence limit was set at 95%. A total of 55 patients were included with the sample size in 40-50 age group less than estimation. The [GR] were found to be significanfly lower in the older age (p=0,01) and the more severe cataract groups (p=0,00). The [GR] were also found to be significantly decreased with the increase of cataract severity in 40-50 age group (p=0,00), 50-60 age group (p=0,01) and >60 age group (p=0,00). In the grade 2 cataract, there were no significant difference of [GR] between age group (p=0.22). This also occured in grade 3 (p=0.23) while in the grade 4, there were significant difference between age group (p=0.01). This also occured in grade 5 (p=0.06). Pearson correlation test showed that there was a strong correlation between [GR] and increase of age (r=0.979) and cataract severity (r=0.969). But in the same cataract grade, the increase of age was not always associated with low [GR] r=0.413,for grade 2, r=0.876 for grade 4 and r=0.731 for grade 5). The conclusion is the increase of age and severity of nuclear cataract In senile cataract, are associated with low [GR]. This association was also found, with the increase of cataract severity in the same age group. However, in the same cataract grade the increase of age is not always associated with a low [GR] Key words: senile cataract -glutathione reductase -nuclear cataract grade -age PENDAHULUANKatarak senilis (Age-related Cataract) merupakan salah satu problem utama kesehatan masyarakat di dunia (1,2). Di negara sedang berkembang, dimana fasilitas kesehatan masih terbatas, terutama fasilitas operasi, buta katarak mencapai hampir setengah dari semua kebutaan (1,(3)(4). Angka kebutaan didunia 0,1-0,4% pada negara maju, sedang di negara sedang berkembang tercatat 0,5-1,5% dengan sebab utama katarak (5,6). Di Indonesia menurut Survei Kesehatan Indra Penglihatan dan Pendengaran Tahun 1993-1996 angka kebutaan 1,5% dengan penyebab utama katarak (0,78%) (7,8).Faktor resiko terjadinya katarak senilis sangat beragam. Faktor personal seperti usia, jenis kelamin, etnis dan keturunan sangat penting. Saat ini penelitian dilakukan pada interaksi antara genetik dan lingkungan yang mempengaruhi genetik tersebut.Faktor lingkungan sangat penting dalam kataraktogenesis. Faktor-faktor tersebut sering terjadi pada masyarakat misal merokok d...

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Section: -Pebruari 2006unclassified

Hubungan Antara Kadar Enzim Glutation Reduktase Dengan Derajat Kekeruhan Inti Lensa

Sulistya

Dewi

Suyuti

et al. 2013

JKB

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“…GPx and GST which catalyze GSH-dependent reduction of LOOH through their peroxidase activity are the major secondary defenses in the lens against ROS induced lipid peroxidation. Resynthesis of GSH from the oxidized form is catalyzed by glutathione reductase, where the necessary NADPH + +H + is produced in pentose pathway of carbohydrates [36]. Glutathione reductase (GR) is a control enzyme glutathione-redox cycle and by maintaining of intracellular levels of GSH can affect cation transport systems, lens hydration, sulfhydryl groups of proteins, and membrane integrity.…”

Section: Defense Against Lipid Peroxidation In the Lensmentioning

confidence: 99%

Role of Lipid Peroxidation in the Pathogenesis of Age-Related Cataract

Kisic¹,

Mirić²,

Zoric³

et al. 2012

Lipid Peroxidation

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“…Умерени степен оксидације протеина чини их осетљивим на протеолизу, док је последица интензивне оксидације формирање нерастворљивих агрегата велике молекулске масе. Променама под утицајем UV зрака подлежу и лентикуларни ензими: глутатион редуктаза, глутатион пероксидаза, супероксид дизмутаза, глицералдехид-3-P дехидрогеназа (G3PD) (137). Активност ензима G3PD се смањује током излагања UVA светлости, а претпоставља се да је то последица оксидације есенцијалних тиол група овог ензима.…”

Section: оксидационе промене изазванеunclassified

“…Они узрокују расипање светлости и смањење видне оштрине (245). Последица оксидационих оштећења је и инактивација ензима (137).…”

Section: биохемијске промене у сочиву током развоја катарактеunclassified

“…Бројна испитивања о утицају UV зрака сунчевог спектра на активност антиоксидационих ензима сочива (глутатион редуктазе, глукозо-6-фосфат дехидргеназе, глутатион пероксидазе и супероксид дисмутазе) указују да UVA зраци сунчевог спектра имају утицај на модификацију сочивних протеина и посебно на смањење специфичне активности ензима хуманог сочива (137). Смањена активност антиоксидационих ензима може бити последица директне UVA фотолизе (117), оксидације реактивним радикалима кисеоника (209) или је последица оба поменута.…”

Section: активност антиоксидационих ензима у сочивима и очној водици unclassified

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Oxidative stress and antioxidant defense elements in the genesis of cataracts

Kisić¹

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Introduction. The immediate cause for the occurrence of cataract is unknown, but oxidative damage and effects of reactive oxygen species are considered important in its etiopathogenesis. Aim. The study was aimed at testing the products oxidative stress and antioxidant protection factors in serum and corticonuclear lenses blocks of patients with different types and different degrees of maturity of agerelated cataract. Material and Methods. Patients. Clinical and biochemical researches were carried out in 101 patients with age-related cataract, 46 women and 55 men. The average age of the group was 72.5 (SD ± 7.98). According to the cataract maturity degree the patients were classified into two groups as follows: cataract senilis incipiens (N=41) and cataract senilis matura (N=60). Serum samples and corticonuclear lenses blocks were obtained to detect the products of lipid peroxidation: conjugated diene, fluorescent lipid peroxidation products and malondialdehyde and activities antioxidative enzymes: superoxide dismutase, catalase, glutathione peroxidase, glutathione reductase and glutathione S-transferase. Results. Significantly higher concentration of diene conjugation in corticonuclear lenses blocks was measured in the patients with cataract senilis incipiens (p<0.001) as well as the intensity of fluorescent iminopropens (p<0.001). Significantly higher concentration of malondialdehyde in corticonuclear lenses blocks (p<0.001) was measured in the patients with cataracta senilis matura. Conclusion. Based on the found lipid peroxidation products measuring results in corticonuclear lenses blocks in the patients with cataract diagnosis, it can be concluded that the lens structure changes caused by lipid peroxidation can, with other risk factors present, influence occurrence and development of cataract. Some cataract types show different lipid peroxidation intensity with the most distinct changes in the cataract wich started as nuclear. Кључне речиОксидациони стрес, катаракта, липидна пероксидација, антиоксидантни ензими.

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Effect of UVA Light on the Activity of Several Aged Human Lens Enzymes

Cited by 23 publications

References 62 publications

Hubungan Antara Kadar Enzim Glutation Reduktase Dengan Derajat Kekeruhan Inti Lensa

Hubungan Antara Kadar Enzim Glutation Reduktase Dengan Derajat Kekeruhan Inti Lensa

Role of Lipid Peroxidation in the Pathogenesis of Age-Related Cataract

Oxidative stress and antioxidant defense elements in the genesis of cataracts

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