Effects of Anti-oxidants on Oxidative Stress: Assessing MDA in Urine Samples

Annamalay, Sundardas Dharmadas

doi:10.15344/2456-8171/2018/135

Cited by 8 publications

(3 citation statements)

References 33 publications

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“…Another study reported that the abdominal Numerous oxygenated compounds, mainly aldehydes such as MDA, are produced during the attack of free radicals on membrane lipoproteins and PUFA. Assay of MDA is probably the most common method for the measurement of lipid peroxidation [50] . This is revealed that there was a significant increase in the levels of MDA and the significant decrease in hepatic SOD, CAT and GSH in the obese rats.…”

Section: Resultsmentioning

confidence: 99%

Ethanol Extract of Punica granatum L. Leaf in Combination with Gallic acid Ameliorates Liver Dysfunction in High-fat Diet-induced Obese Rats

Rao¹,

Krishnamurthy²

2019

pharmaceutical-sciences

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Rao et al.: Hepatoprotective Activity of Punica granatum L. Leaf Extract in combination with Gallic acid The present study was designed to evaluate the hepatoprotective activity of ethanol extract of Punica granatum L. leaf in association with gallic acid in high-fat diet-induced animal model. Total 30 rats were used. These were divided into 5 groups of 6 animals each. Group 1 was given normal diet and was treated as the control. Group 2 was given a high-fat diet and was considered as induced group. Groups 3, 4 and 5 were given high-fat diet along with ethanol extract of Punica granatum L. leaf (200 mg/kg), ethanol extract of Punica granatum L. leaf+gallic acid (100+10 mg/kg) and orlistat (22 mg/kg), which constituted the treatment groups. The total period of the study was 8 w. At the end of the study, activities of alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, total protein, albumin, globulin and albumin/globulin ratio were measured. The antioxidant defence activities like superoxide dismutase, catalase and reduced glutathione were analysed together with lipid peroxidation in the liver tissue. In addition, histology of liver was examined. The high-fat diet caused alternation in the activities of markers of liver function along with changes in histology of the liver. The high-fat diet increased lipid peroxidation and decreased antioxidant defence system. The alterations induced by the high-fat diet were restored more effectively by the ethanol extract of Punica granatum L. leaf+gallic acid than the ethanol extract of Punica granatum L. leaf alone or orlistat.

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Section: Resultsmentioning

confidence: 99%

Ethanol Extract of Punica granatum L. Leaf in Combination with Gallic acid Ameliorates Liver Dysfunction in High-fat Diet-induced Obese Rats

Rao¹,

Krishnamurthy²

2019

pharmaceutical-sciences

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“…It was noted that it had increased in all rat brain regions after aspartame consumption and had serious effects on cellular functions [12]. It produces mediators such as MDA which is a valid biomarker for oxidative stress [13]. One of the main components of the antioxidant factors in living cells is GSH.…”

Section: Discussionmentioning

confidence: 99%

Can Aspartame Induce Reversible Cerebellar Changes?

Youssef

Ahmed

El-Dken

et al. 2023

Preprint

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Aspartame is a synthetic sweetener that is unlikely to have a negative impact on the cerebellar cortex. The current study was designed to evaluate the histopathological changes in the cerebellar cortex of aspartame-treated albino rats and the possibility of recovery from aspartame induced cerebellar injury. Three groups of six mature male albino rats, totaling 18, were allocated. Daily doses of distilled water were given to the control group. Group 2 (ASP group): received 250 mg/kg ASP by oral rote for 12 weeks. Group3 (Recovery) received 250 mg/kg/day aspartame for eight weeks, then a daily dosage of distilled water equal to the aspartame dose for the next 6 weeks. The rats were anesthetized, and their cerebella were dissected for immunohistochemical and histological studies. Studies in morphometry and statistics were carried out. Nitric oxide (NO), reduced glutathione (GSH), and malondialdehyde (MDA) levels were assessed in the cerebellum tissue. When compared to the control group, there was a very significant rise in MDA and NO levels and a reduction in GSH levels in the aspartame group. MDA and NO levels were decreased associated with a significant increase in GSH level compared to the aspartame group in the recovery group. Cerebellar cortex of aspartame group showed features of neurodegeneration, and apoptosis. The latter features were decreased in the recovery group. In conclusion, aspartame consumption has reversible deleterious effect on cerebellar cortex.

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“…7 MDA is claimed to become more mutagenic, then tumorigenic and also more reactive. 8 The simple and cost-effective process makes MDA routinely used for clinical analysis. 9 MDA in plasma, serum and urine samples has been used as a biomarker of oxidative stress.…”

Section: Introductionmentioning

confidence: 99%

Correlation of Serum Nitric Oxide and Urine Malondialdehyde Levels in Non-Hemodialysis Chronic Kidney Disease Patients

Purwati

Mustika

Hakim

et al. 2022

Mol Cell Biomed Sci

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Background: In 2017, about 1.2 million people died because of Chronic Kidney Disease (CKD). Patients with CKD are known to have increased levels of oxidative stress which leads to decrease in NO production. NO is a highly reactive signaling molecule and a major determinant of vascular homeostasis. Thus, the decreased NO can be a risk factor for the development of atherosclerosis and increased cardiovascular risk. Meanwhile, Malondialdehyde (MDA) is known as excellent biomarker for oxidative stress. This study aims to determine the correlation of serum total nitric oxide (NO) and urine MDA levels in non-hemodialysis CKD patients.Materials and Methods: This study was an observational clinical study with a cross sectional design. Fourty-nine CKD subjects were selected by consecutive sampling. The samples for laboratory tests were collected from urine. MDA concentration was measured using the High-Performance Liquid Chromatography (HPLC) kit. NO concentration was measured with Griess reaction method and Total Nitric Oxide Parameter kit. The data were analyzed using the Statistic Package for Social Science (SPPS) software version 16.Results: The data showed significant negative correlations between MDA with NO (r=-0.294; p=0.041).Conclusion: There was a correlation between serum total NO and urine MDA levels in non-hemodialysis CKD patients.Keywords: chronic kidney disease, malondialdehyde, nitric oxide, non-hemodialysis

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Effects of Anti-oxidants on Oxidative Stress: Assessing MDA in Urine Samples

Cited by 8 publications

References 33 publications

Ethanol Extract of Punica granatum L. Leaf in Combination with Gallic acid Ameliorates Liver Dysfunction in High-fat Diet-induced Obese Rats

Ethanol Extract of Punica granatum L. Leaf in Combination with Gallic acid Ameliorates Liver Dysfunction in High-fat Diet-induced Obese Rats

Can Aspartame Induce Reversible Cerebellar Changes?

Correlation of Serum Nitric Oxide and Urine Malondialdehyde Levels in Non-Hemodialysis Chronic Kidney Disease Patients

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