Effects of astaxanthin on plasma membrane function and fertility of boar sperm during cryopreservation

Guo, Hai-tao; Wang, Jing-Ran; Sun, Liang-zhen; Jin, Xiaohu; Shi, Xueying; Lin, Jingyi; Shun-li, Yue; Zhou, Jiabo

doi:10.1016/j.theriogenology.2021.01.007

Cited by 22 publications

(14 citation statements)

References 22 publications

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“…AXN supplementation showed improved and protected sperm motility, viability, membrane integrity, and DNA during liquid preservation of boar [115] and ram [116] semen. Similar findings were observed during freeze-thaw procedures using an AXN-supplemented freezing extender in boar [117], dog [118],…”

Section: A C C E P T E D a R T I C L Esupporting

confidence: 81%

Role of antioxidants in fertility preservation of sperm — A narrative review

Qamar¹,

Naveed²,

Raza³

et al. 2023

Anim Biosci

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Male fertility is affected by multiple endogenous stressors, including reactive oxygen species (ROS), which greatly deteriorate it the fertility. However, physiological levels of ROS are required by sperm for the proper accomplishment of different cellular functions including proliferation, maturation, capacitation, acrosomal reaction, and fertilization. Excessive ROS production creates an imbalance between ROS production and neutralization resulting in oxidative stress (OS). OS causes male infertility by impairing sperm functions including reduced motility, deoxyribonucleic acid (DNA) damage, morphological defects, and enhanced apoptosis. Several in-vivo and in-vitro studies have reported improvement in quality-related parameters following the use of different natural and synthetic antioxidants. In this review, we focus on the causes of OS, ROS production sources, mechanisms responsible for sperm damage, and the role of antioxidants in preserving sperm fertility.

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Section: A C C E P T E D a R T I C L Esupporting

confidence: 81%

Role of antioxidants in fertility preservation of sperm — A narrative review

Qamar¹,

Naveed²,

Raza³

et al. 2023

Anim Biosci

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“…Astaxanthin significantly improved porcine sperm motility, acrosome integrity and plasma membrane integrity during storage. These results are consistent with Guo et al (2021), although those authors investigated the effect of astaxanthin on cryopreservation of porcine sperm (Guo et al, 2021). In addition, the addition of astaxanthin to rat semen significantly reduces ROS production; it positively affects male fertility by increasing sperm concentration, motility, normal sperm morphology and linear motility velocity (Comhaire et al, 2005; Mortazavi et al, 2014).…”

Section: Discussionsupporting

confidence: 92%

Astaxanthin improves preserved semen quality at 17°C by enhancing sperm antioxidant and motility properties

Pang

et al. 2022

Reprod Domestic Animals

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Porcine sperm is rich in polyunsaturated fatty acids; therefore, it is highly susceptible to oxidative damage during storage. Inhibition of oxidative stress during preservation is essential for maintaining sperm motility. Astaxanthin is a potent antioxidant used in the cosmetic and pharmaceutical industries. This study aimed to explore the effect of supplementing astaxanthin as an extender of porcine semen preservation dilutions at 17°C. Various concentrations of astaxanthin were added to diluted porcine semen at 17°C. We performed computer-assisted semen analysis, evaluation of plasma membrane integrity and acrosome integrity, and measurement of total antioxidant activity, malondialdehyde (MDA) content, reactive oxygen species levels, superoxide dismutase (SOD) activity, catalase (CAT) activity, glutathione peroxidase (GSH-PX) activity and sperm motility parameters. Compared with the control group, the addition of 0.25 μg/ml astaxanthin group significantly improved sperm motility parameters stored on the fifth day; these were increased levels of sperm SOD, GSH-PX and CAT (p < .05), increased sperm adenosine trisphosphate and lactate dehydrogenase levels and decreased sperm MDA levels (p < .05). These findings suggest that adding 0.25 μg/ml of astaxanthin improves the quality of porcine semen stored at 17°C. Our findings provide theoretical support for developing new protective agents critical for preserving pig semen at 17°C.

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“…The study was performed using semen from six Large White boars purchased from a commercial farm (Sanyuan Livestock Industry Company). The sperm cryopreserved procedure was based on the protocol described by Guo et al (2021) In brief, the sperm‐rich fractions of ejaculates were diluted in Modena and cooled down to 15°C for 120 min, and centrifuged at 800 × g for 10 min. Pellets were subsequently recovered and diluted to 1.0 × 10 9 spermatozoa/ml in freezing extender I.…”

Section: Methodsmentioning

confidence: 99%

Ameliorative effect of silymarin on the quality of frozen–thawed boar spermatozoa

Shun-li

Wang

et al. 2022

Reprod Domestic Animals

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Cryopreservation, which is a vital method for efficient, economical, long-term storage of sperm, has an irreplaceable advantage in breeding excellent breeding stocks, protecting endangered species and international germplasm exchange, while gene banks and planning of artificial insemination (AI) centres are also using a large amount of frozen-thawed semen (Pezo et al., 2019). Yet, only 1% of AI worldwide used frozen-thawed boar semen because boar sperm is more susceptible to the stress caused by low temperature, osmotic shock, reactive oxygen species (ROS) production and the toxicity of the cryoprotectants (De Andrade et al., 2022).

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Effects of astaxanthin on plasma membrane function and fertility of boar sperm during cryopreservation

Cited by 22 publications

References 22 publications

Role of antioxidants in fertility preservation of sperm — A narrative review

Role of antioxidants in fertility preservation of sperm — A narrative review

Astaxanthin improves preserved semen quality at 17°C by enhancing sperm antioxidant and motility properties

Ameliorative effect of silymarin on the quality of frozen–thawed boar spermatozoa

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