Effects of collagen peptides intake on skin ageing and platelet release in chronologically aged mice revealed by cytokine array analysis

Abstract

Action mechanisms underlying various biological activities of collagen peptides (CPs) remained to be elucidated. Cytokines may play an important role in mediating these health benefits of CPs. This study aimed to systemically examine the cytokines in skin and blood regulated by CPs intake. Thirteen‐month‐old female Kunming mice were administered with CPs for 2 months (0 or 400 mg/kg bodyweight/day). The cytokines in skin and plasma were analysed using a 53‐cytokine array and corresponding ELISA kits. In skin, CPs intake significantly down‐regulated placenta growth factor (PIGF‐2), insulin‐like growth factor (IGF)‐binding protein (IGFBP) ‐2 and IGFBP‐3, and up‐regulated platelet factor 4 (PF4), serpin E1 and transforming growth factor (TGF)‐β1. CPs treatment also increased the type I collagen mRNA and protein levels and improved the aged skin collagen fibres. In plasma, nine cytokines were significantly down‐regulated by CPs intake compared to the model group: fibroblast growth factor (FGF)‐2, heparin‐binding (HB) epidermal growth factor (EGF)‐like growth factor (HB‐EGF), hepatocyte growth factor (HGF), platelet‐derived growth factor (PDGF)‐AB/BB, vascular endothelial growth factor (VEGF), chemokine (C‐X‐C motif) ligand 1 (KC), matrix metalloproteinase (MMP)‐9, interleukin (IL)‐1α and IL‐10; 2 cytokines were significantly up‐regulated, including TGF‐β1 and serpin F1. Furthermore, CPs intake significantly decreased the level of platelet release indicators in the plasma and washed platelets, including PF4, granule membrane protein (GMP)‐140, β‐thromboglobulin and serotonin. These results provide a mechanism underlying anti‐skin ageing by CPs intake and highlight potential application of CPs as a healthcare supplement to combat cancer and cardiovascular disease by inhibiting platelet release.

A novel di‐peptide Met‐Glu from collagen hydrolysates inhibits platelet aggregation and thrombus formation via regulation of Gq‐mediated signaling

“…Due to the high turnover of the platelet in the body and its essential role in hemostasis and thrombosis, the platelet has long been a primary target for therapeutic intervention for the prevention of occlusive thrombotic events (Yeung et al, 2018). Our previous study has shown that oral administration of collagen hydrolysates significantly downregulated nine cytokines that are highly expressed in elder mice platelets to inhibit their activation (Song et al., 2018). However, the peptide sequence with antiplatelet aggregation activity remained unknown.…”

“…However, to the best of our knowledge, most of these studies have focused on the function evaluation and there is scanty report on the sequence of collagen peptides as well as their molecular regulatory mechanisms. Our previous study has shown that oral administration of collagen hydrolysates could downregulate nine cytokines significantly, which were highly expressed in activated platelet (Song, Zhang, Luo, Zhang, & Li, 2018). However, the peptide sequence and the anti‐thrombotic effects in vivo and the mechanism remained unknown.…”

Effect of Orally Administered Collagen Peptides from Bovine Bone on Skin Aging in Chronologically Aged Mice

“…Collagen peptides (CPs) have been widely reported to exert beneficial effects on photoaging skin, but few studies was carried out to evaluate their effect on chronologically aged skin. In present study, 13-month-old Kunming mice, equivalent to 45 years old of human life [ 29 ], were employed to investigate the effect of CPs on chronologically aged skin. In several previous clinical trials, a daily dose of 2.5 g or 5 g of CPs has been employed in adult subjects, and these doses were considered to be safe [ 16 , 17 , 30 ].…”

Chicken Combs and Wattles as Sources of Bioactive Peptides: Optimization of Hydrolysis, Identification by LC-ESI-MS2 and Bioactivity Assessment

“…Chicken combs and wattles were ground and homogenized at the ratio of 1:1 (w/w). This The oral ingestion of collagen peptides has been correlated with decreasing levels of platelet release indicators in plasma and increasing levels of type I collagen, which, in turn, improves aged skin collagen fibers [26]. This was particularly attributed to an enrichment of Pro-Hyp derived from Gly-Pro-Hyp hydrolysis, which was deposited in the skin [27].…”