Effects of dietary vitamin C on growth, flesh quality and antioxidant capacity of juvenile golden pompanoTrachinotus ovatus

Abstract

To investigate the effects of dietary vitamin C on growth, flesh quality and antioxidant capacity of juvenile golden pompano Trachinotus ovatus, a 56-day feeding trial with five graded levels of dietary VC (D1: 11.69, D2: 34.89, D3: 59.10, D4: 114.26 and D5: 227.93 mg VC per kg of diet) was performed on 375 fish (triplicate groups of 25 fish per diet, initial weight 13.57 ± 0.09 g). Results showed that fish of D3 group exhibited the maximum specific growth rate (SGR) and the highest liver enzymatic activities of catalase (CAT) and glutathione peroxidase (GSH-PX), which were consistent with the expression levels of cat and gsh-px. Besides, the D3 group also showed higher contents of protein and lipid, and lower cooking loss, drip loss and malondialdehyde content in muscle than D1 group. The docosahexaenoic acid proportion in muscle increased with increasing dietary VC levels. Furthermore, the lowest expression levels of carnitine palmitoyltransferase1 (cpt1) and peroxisome proliferator-activated receptor α (pparα) were detected in livers of D3 group. The optimum dietary VC level was 49.73 mg/kg from the broken-line analysis based on the SGR, in which better growth performance, antioxidative ability and flesh quality were observed in T. ovatus juveniles. K E Y W O R D Santioxidative ability, flesh quality, growth performance, Trachinotus ovatus, vitamin C | 2857 ZHANG et Al.

Impacts of dietary konjac glucomannan supplementation on growth, antioxidant capacity, hepatic lipid metabolism and inflammatory response in golden pompano (Trachinotus ovatus) fed a high fat diet

“…Fishmeal, fermented soybean meal and soy protein concentrate were used as the major protein sources, and a mixture of palm oil, rapeseed oil and perilla oil (3:1:1, by volume) was used as the lipid source in the experiment diets. The feeds were produced as described in detail previously (Zhang et al, 2019a). All dry ingredients were ground into fine powder, sieved through size 40 mesh (425 micron), and mixed thoroughly before adding the vegetable oil mixtures and the appropriate amount of water.…”

“…All the enzymatic activities and nonenzymatic factor contents were calculated according to the manufacturer's instructions 2.6. Serum and liver antioxidant parameters Serum superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and catalase (CAT) activities, total antioxygenic capacity (T-AOC), and malondialdehyde (MDA) concentration were analyzed using commercial assay kits (Nanjing Jiancheng Bioengineering Co. Ltd.) as described previously (Zhang et al, 2019a). Malondialdehyde was determined according to the thibabituric acid method (TAB) introduced by commercial kits.…”

“…Pompanos, which constitute around 21 species of the genus Trachinotus in the Carangidae or jack family, are carnivorous marine fish that mainly feed on zooplankton, shellfish and small fish (Zhang et al, 2019a). Most Trachinotus species have desirable taste characteristics and are thus valued as food fish with limited commercial fisheries, and some species that exhibit rapid growth, high disease resistance and suitability for cage culture have made pompano species, such as T. ovatus and T. blochii, promising candidates for intensive aquaculture in Southern China with annual production of around 120,000 tons (Li et al, 2020;Tan et al, 2017).…”

“…Most Trachinotus species have desirable taste characteristics and are thus valued as food fish with limited commercial fisheries, and some species that exhibit rapid growth, high disease resistance and suitability for cage culture have made pompano species, such as T. ovatus and T. blochii, promising candidates for intensive aquaculture in Southern China with annual production of around 120,000 tons (Li et al, 2020;Tan et al, 2017). Dietary protein requirement of pompano at around 50 % is relatively high (Wang et al, 2020;Zhang et al, 2019a), so that improving utilization of dietary lipid as an economical source of energy to spare protein for somatic growth is crucial for viable farming of pompano. The optimum dietary lipid level for juvenile pompano has been suggested to be 5-12 % of diet (Tan et al, 2013), and commercial feed produced for this species in China contains 9 % lipid.…”

Evaluation of yeast hydrolysate as a substitute to dietary fish meal of| juvenile Jian carp ( Cyprinus carpio var. Jian): Protein synthesis via TOR pathway

“…Early study reported that the rates of hypertrophy and hyperplasia of muscle fibre change between different strains of the same species (Weatherley et al, 1979) and are influenced by farmed conditions such as diet (Kiessling et al, 1991;Lo´pez-Albors et al, 2010). More research evaluated the effects of feed additives on flesh quality of fish in wild and farmed in different species, such as Turbot (Psetta maxima) (Regost et al, 2003), blunt snout bream (Megalobrama amblycephala) (Cai et al, 2018) and golden pompano (Trachinotus ovatus) (Zhang et al, 2019).…”