Effects of different antibiotics on the uterine contraction and the expression of aquaporin 5 in term pregnant rat

Abstract

Aquaporin (AQP) water channels are small hydrophobic integral membrane proteins. AQP5 expression, which is regulated by oxytocin, showed a dramatic down-regulation at the term and preterm uterus. Since antibiotics are among the drugs to treat intrauterine infections, our aim was to study the effects of antibiotics on AQP5 and uterine contractility on 22-day pregnant rats. The change in uterine AQP5 expression was investigated by PCR and Western blot techniques. Uterine contractility was tested in an organ bath system. 7 days of pre-treatment with amoxicillin or single dose of fosfomycin decreased the AQP5 protein level, while 7 days of treatment with doxycycline had no effect. Fosfomycin or amoxicillin pre-treatments enhanced, while doxycycline pre-treatment did not alter the oxytocin-induced contractions. Amoxicillin and fosfomycin may sensitize the uterus to oxytocin via the reduction of AQP5 expression. This synergism might have importance during the pharmacotherapy of infection-related preterm birth.

Histopathological changes in liver, kidney and teratogenic effects of mice on exposure to mosquito repellent

“…Day 0 of pregnancy was determined by presence of a vaginal plug. 14,15 All pregnant mice were divided into 3 groups. Each group consisted of 6 mice that were randomly chosen and then acclimatized for 7 days.…”

The role of the aquaporin 5 in the function of late pregnant rat uterus: pharmacological studies

“…The uterine tissues showed enhanced contractility responses to oxytocin as a result of amoxicillin pretreatment, in the in vitro isolated organ experiment. (Csányi et al, 2018) This phenomenon might explain the ineffectiveness amoxicillin and other beta-lactam antibiotics in the prevention of preterm delivery. (Lee et al, 2016) AQP5 mRNA level was significantly elevated while the protein level declined as a result of fosfomycin pretreatment.…”