Effects of hydrogenated TiO&lt;sub&gt;2&lt;/sub&gt; nanotube arrays on protein adsorption and compatibility with osteoblast-like cells

Lu, Ran; Wang, Caiyun; Wang, Xin; Wang, Yuji; Wang, Na; Chou, Joshua; Li, Tao; Zhang, Zhenting; Ling, Yi; Su, Chen

doi:10.2147/ijn.s155532

Cited by 34 publications

(26 citation statements)

References 53 publications

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“…It has been reported that protein adsorption on TiO 2 nanotube surfaces is dependent on surface properties. 50,51 The TiO 2 nanotubes with larger diameters possessed greater interaction energies, leading to better protein adsorption and thus regulating cell responses. 52 In addition to the influence of nanotube diameter, it is possible that the bioactivity of 105 nm TiO 2 nanotubes, i.e.…”

Section: Discussionmentioning

confidence: 99%

Titanium dioxide nanotubes of defined diameter enhance mesenchymal stem cell proliferation via JNK- and ERK-dependent up-regulation of fibroblast growth factor-2 by T lymphocytes

et al. 2018

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Long-term clinical success of a titanium implant not only depends upon osseointegration between implant and bone surface but also on the response of host immune cells. Following implantation of biomaterials, an inflammatory response, including T lymphocyte response, is ostensibly initiated by implant-cell interaction. However, little is known about the responses of T lymphocytes to titanium dioxide nanotubes. The present study aimed to explore the effect of titanium dioxide nanotubes on T lymphocytes in vitro and its biological consequences. The results of the present study showed that titanium dioxide nanotubes with diameter of 30–105 nm were non-cytotoxic to T lymphocytes, and the 105 nm titanium dioxide nanotube surface specifically possessed an ability to activate T lymphocytes, thus increasing DNA synthesis and cell proliferation. In addition, the 105 nm titanium dioxide nanotubes significantly activated the expression of FGF-2 gene and protein in T lymphocytes although smaller nanotubes (i.e. those with diameters of approximately 30 and 70 nm) had little effect on this. The present study investigated the mechanism by which 105 nm nanotubes stimulated FGF-2 expression in T lymphocytes by blocking key MAPK pathways. The inhibitors of JNK1/2/3 and ERK1/2 significantly inhibited 105 nm titanium dioxide nanotubes-induced FGF-2 expression. Corresponding to the increased expression of FGF-2, only the supernatant from T lymphocytes cultured on 105 nm nanotubes stimulated human mesenchymal stem cell proliferation. FGF-2 blocking antibody partially reversed the increased proliferation of human mesenchymal stem cells, supporting the role of T lymphocyte-derived FGF-2 in enhanced human mesenchymal stem cell proliferation. This suggests a significant role of T lymphocyte-titanium dioxide nanotube interaction in the proliferation of human mesenchymal stem cells, which is pivotal to the formation of new bone following implant placement.

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Section: Discussionmentioning

confidence: 99%

Titanium dioxide nanotubes of defined diameter enhance mesenchymal stem cell proliferation via JNK- and ERK-dependent up-regulation of fibroblast growth factor-2 by T lymphocytes

et al. 2018

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“…Park et al reported that the difference of nanotube diameters can severely influence cell adhesion and spreading of mesenchymal stem cells (Park, Bauer, Mark, & Schmuki, 2007). Lu et al proved that the hydrogenated TiO 2 nanotubes upon thermal treatment could greatly prove the loading capacity of bioactive molecules and subsequent MG‐63 cell proliferation (Lu et al, 2018). Chen et al modified TiO 2 nanotube arrays with functional proteins (bone morphogenetic protein 2 and sclerostin antibody) to promote osteoblast differentiation of mesenchymal stem cells and bone fracture recovery (Chen et al, 2019; Lai et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

Adsorption of serum proteins on titania nanotubes and its role on regulating adhesion and migration of mesenchymal stem cells

Zhang

Bai

et al. 2020

J Biomedical Materials Res

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Migration and differentiation of bone marrow‐derived mesenchymal stem cells (BMSCs) is an important biological process in tissue regeneration. Nanostructured titanium materials are believed to play a fundamental role in dental and orthopedic applications. However, the protein adsorption on nanostructured titanium materials and its correlation with the subsequent cell behaviors have not been studied. In this work, the titania nanotube arrays with different tubular diameters ranging from 27.3 to 88.2 nm were fabricated by using an electrochemical etching method. The adsorbed amounts and types of cell adhesion‐related proteins (such as fibronectin, vitronectin, and laminin) from serum were investigated, revealing that these proteins were preferred to bind onto the surface with nanotubes of a smaller diameter. Adhesion and migration of BMSCs were studied as a function of different nanotube diameters in the presence or absence of serum proteins. Compared with the nanotube surface with a larger tubular diameter (88.2 nm), the surface with a smaller one could better support BMSCs in terms of adhesion and spreading. The pre‐adsorbed serum proteins significantly enhanced adhesion and migration abilities of BMSCs. However, the adequate interactions between cells and serum proteins on the nanotubes surface with smallest nanotubes in diameter weakened cell mobility. Arrangement of cytoskeleton and expressions of key genes and proteins were studied, revealing that the nanostructured surfaces and pre‐adsorbed proteins jointly mediated the adhesion and migration of BMSCs.

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“…TiO 2 nanotubes are of great interest due to their excellent physical, mechanical and biological properties. Previous studies have reported that nanotubes enhance the protein adsorption and osteoblast cell attachment which improves bioactivity [23,24]. In this study, we fabricated nanotubes on the surface of both CP-Ti and Ti-6Al-4V using experimental different conditions.…”

Section: Discussionmentioning

confidence: 99%

Bone marrow stromal cells interaction with titanium; Effects of composition and surface modification

et al. 2019

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Inflammation and implant loosening are major concerns when using titanium implants for hard tissue engineering applications. Surface modification is one of the promising tools to enhance tissue-material integration in metallic implants. Here, we used anodization technique to modify the surface of commercially pure titanium (CP-Ti) and titanium alloy (Ti-6Al-4V) samples. Our results show that electrolyte composition, anodization time and voltage dictated the formation of well-organized nanotubes. Although electrolyte containing HF in water resulted in nanotube formation on Ti, the presence of NH4F and ethylene glycol was necessary for successful nanotube formation on Ti-6Al-4V. Upon examination of the interaction of bone marrow stromal cells (BMSCs) with the modified samples, we found that Ti-6Al-4V without nanotubes induced cell proliferation and cluster of differentiation 40 ligand (CD40L) expression which facilitates B-cell activation to promote early bone healing. However, the expression of glioma associated protein 2 (GLI2), which regulates CD40L, was reduced in Ti-6Al-4V and the presence of nanotubes further reduced its expression. The inflammatory cytokine interleukin-6 (IL-6) expression was reduced by nanotube presence on Ti. These results suggest that Ti-6Al-4V with nanotubes may be suitable implants because they have no effect on BMSC growth and inflammation.

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Effects of hydrogenated TiO<sub>2</sub> nanotube arrays on protein adsorption and compatibility with osteoblast-like cells

Cited by 34 publications

References 53 publications

Titanium dioxide nanotubes of defined diameter enhance mesenchymal stem cell proliferation via JNK- and ERK-dependent up-regulation of fibroblast growth factor-2 by T lymphocytes

Titanium dioxide nanotubes of defined diameter enhance mesenchymal stem cell proliferation via JNK- and ERK-dependent up-regulation of fibroblast growth factor-2 by T lymphocytes

Adsorption of serum proteins on titania nanotubes and its role on regulating adhesion and migration of mesenchymal stem cells

Bone marrow stromal cells interaction with titanium; Effects of composition and surface modification

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