BACKGROUND: Infant care activities can induce musculoskeletal disease. However, little is known about the biomechanical joint load during lifting-up of an infant. OBJECTIVE: The aim of this study was to investigate normalized maximum moment during lifting-up of infant dummies weighing 4.6 kg, 7.6 kg, and 9.8 kg. METHODS: Six healthy young subjects participated in our study. All subjects performed lifting-up activities of dummies to shoulder height with their feet apart and natural postures in their comfortable speed. Three-dimensional reflective marker trajectories and ground reaction forces were used as input to calculate joint moments using a full body musculoskeletal model. Joint moments were normalized by each subject’s body mass. Friedman’s test was performed to compare mean differences of normalized joint moments for lifting up three dummy weights. RESULTS: Lumbar joint had the greatest normalized joint moment. Lumbar and hip extension moments were significantly increased with dummy weight (P< 0.05). In contrast, knee extension and ankle plantarflexion moment were not significantly affected by dummy weight (P> 0.05). CONCLUSIONS: These results indicate that the lumbar joint plays the most important role in infant lifting-up motion and that the load of lumbar and hip joint should be reduced when lifting a heavier infant. These results could contribute to the development of an effective lifting strategy and an assisting device for lifting an infant.