Effects of Intracerebroventricular Micro-injection of Kaempferol on Anxiety: Possible GABAergic Mechanism Involved

Zarei, Mohammad; Sarihi, Abdolrahman; Ahmadimoghaddam, Davoud; Soltani, Erfan

doi:10.32592/ajnpp.2021.8.2.108

Cited by 2 publications

(2 citation statements)

References 23 publications

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“…The flavonols kaempferol, quercetin, and myricetin also showed evidence for an anxiolyticlike effect. The mechanisms of action of these flavanols are uncertain; intracerebroventricular injection of kaempferol reduces anxiety-like behavior in male rats by a GABA A receptor-dependent mechanism (Zarei et al, 2021). However, intraperitoneal injections of these three flavanols failed to affect anxiety-like behavior, while administration per os decreased it, suggesting that these flavanols are pro-drugs which are metabolized before they reach the brain (Vissiennon et al, 2012).…”

Section: Discussionmentioning

confidence: 99%

Flavonoids as anxiolytics in animal tests: Bibliometry and meta-analysis of the effects of flavonoids on anxiety-like behavior in animal tests

Machado,

Araújo,

Lima-Maximino

et al. 2024

Preprint

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FigureFlavonoids are natural secondary compounds of plants with a basic composition derived from polyphenols that can produce a plethora of different neurochemical effects, some of which are relevant to anxiety disorders. As such, many flavonoids have been evaluated in behavioral screens in preclinical research on anxiolytics. Given the many different molecular subclasses of flavonoids, the many different molecular targets that have been proposed for these compounds, and the different research priorities that arise in preclinical research, we sought to map the potential of flavonoids as anxiolytics by bibliometric analysis and a meta-analysis of animal tests using these compounds. Bibliometric analysis suggest that the field is highly concentrated on few research groups that are mostly located in the Global South, suggesting the need to improve international collaborations. The themes which emerged in the bibliometric analysis are driven by the exploratory steps of pharmacological research, including finding anxioselective effects and looking for dose-response patterns; this suggests that the field, as a whole, could benefit from more mechanistic and confirmatory research. The meta-analysis showed strong evidence for an anxiolytic-like effect of flavonoids on animal tests, including assays made in rats, mice, and zebrafish (SMD = -1.4398, 95%CI[-1.7319, - 1.1477]). Subgroup analysis suggested that this effect is present in acute treatment (SMD = -1.14, 95%CI[-1.36, -0.93]), but not after chronic treatment (SMD = -1.96, 95%CI[-4.93; 1.01]). For all molecule subclasses included in the study, only isoflavones, glycoside derivatives, and flavanolignans did not show evidence of an anxiolytic-like effect, although the low number of studies including these subclasses is low. We finish with a set of recommendations for preclinical research on the anxiolytic potential of flavonoids.

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Section: Discussionmentioning

confidence: 99%

Flavonoids as anxiolytics in animal tests: Bibliometry and meta-analysis of the effects of flavonoids on anxiety-like behavior in animal tests

Machado,

Araújo,

Lima-Maximino

et al. 2024

Preprint

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“…In addition, its administration protected against Chlorpyrifos-induced oxidative stress and memory deficits in rats via GSK3β-Nrf2 signaling pathway and increased the activities of antioxidant enzymes and AChE ( Hussein et al, 2018 ). Furthermore, the intracerebroventricular Micro-injection of Kaempferol reduced anxiety through the GABAergic mechanism by binding to specific sites of benzodiazepines ( Zarei et al, 2021 ). However, in H 2 O 2 conditions, it was observed that the group received a 250 mg/kg dose of M. rotundifolia extract had significantly less locomotor activity.…”

Section: Discussionmentioning

confidence: 99%

Mentha rotundifolia (L.) Huds. aqueous extract attenuates H2O2 induced oxidative stress and neurotoxicity

Boualam

Bouhaddou²,

Sobeh³

et al. 2023

Front. Neurosci.

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IntroductionOxidative stress plays a causal role in neurodegenerative diseases. The aim of this study is to evaluate the antioxidant and neuroprotective effects of Mentha rotundifolia (L.) Huds (M. rotundifolia), a widely used Moroccan plant in traditional medicine.MethodsThe chemical composition of M. rotundifolia aqueous extract was analyzed by liquid chromatography coupled to mass spectrometry (LC-MS). 2,2-diphenyl 1-picrylhydrazyl (DPPH) and 2,2′-azino-bis 3-ethylbenzothiazoline-6-sulfonic acid (ABTS+) assays were used to assess its in vitro antioxidant activity. H2O2 was utilized to induce oxidative stress and neurotoxicity in vivo. Behavioral changes were evaluated using Open Field, Y-maze and Rotarod tests. Hyperalgesia was assessed using the tail immersion test.Results and discussionThe LC-MS/MS analysis revealed high content of kaempferol glucuronide (85%) at the extract. IC50 values of the DPPH and ABTS were 26.47 and 41.21 μg/mL, respectively. Pre-treatments with M. rotundifolia extract attenuated the behavioral changes induced by H2O2. In addition, the latency of tail withdrawal increased significantly in the treated groups suggesting central analgesic effect of M. rotundifolia extract. Moreover, the extract attenuated the deleterious effects of H2O2 and improved all liver biomarkers. The obtained results suggested that M. rotundifolia had remarkable antioxidant and neuroprotective effects and may prevent oxidative stress related disorders.

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Effects of Intracerebroventricular Micro-injection of Kaempferol on Anxiety: Possible GABAergic Mechanism Involved

Cited by 2 publications

References 23 publications

Flavonoids as anxiolytics in animal tests: Bibliometry and meta-analysis of the effects of flavonoids on anxiety-like behavior in animal tests

Flavonoids as anxiolytics in animal tests: Bibliometry and meta-analysis of the effects of flavonoids on anxiety-like behavior in animal tests

Mentha rotundifolia (L.) Huds. aqueous extract attenuates H2O2 induced oxidative stress and neurotoxicity

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