Effects of Lactobacillus buchneri inoculation or 1-propanol supplementation to corn silage on the performance of lactating Holstein cows

Abstract

-The objective was to evaluate the effects of corn silage supplemented with 1-propanol or inoculated with L. buchneri on the ruminal fermentation profile, digestibility, and production traits of lactating Holstein cows. Whole-corn plants were harvested at 350 g/kg dry matter (DM) and packed in nine bag silos (13 t/silo). At ensiling, two treatments were applied: control (no additive; six silos) and L. buchneri inoculation with 1 × 10 5 cfu/g (three silos). Feeding started after 247 days of storage; one L. buchneri and two control silos were opened in each experimental period. Twenty-one multiparous Holstein cows (~33 kg/day of milk) were allocated to seven balanced 3 × 3 Latin squares with 21-day periods (14 days of adaptation). The experimental diets contained (DM basis): 80 g/kg cottonseed, 95 g/kg citrus pulp, 180 g/kg soybean meal, 90 g/kg corn grain (ground), 25 g/kg minerals and vitamins premix, and 530 g/kg of corn silage. Source of corn silage was the only difference between experimental treatments: control, L. buchneri, or control silage supplemented with 1-propanol (10 g/kg of diet DM). The 1-propanol was dissolved in water (1:1) and sprinkled onto the ration during mixing, immediately before each feeding. Dry matter intake, nutrient digestibility, milk yield, and composition were not affected by treatments. Cows fed 1-propanol had greater concentrations of 1-propanol in the rumen fluid, higher concentration of glucose, and lower concentration of non-esterified fatty acids in blood plasma. Corn silage inoculated with L. buchneri at 1 × 10 5 cfu/g does not affect silage fermentation, ruminal fermentation profile, or milk production. Supplementation of 1-propanol at 10 g/kg affects the ruminal fermentation profile without affecting feed intake and milk production of mid-lactating dairy cows.Key Words: aerobic stability, alcohol, heterofermentative inoculant, voluntary feed intake Revista Brasileira de Zootecnia

Meta-analysis of effects of inoculation with Lactobacillus buchneri, with or without other bacteria, on silage fermentation, aerobic stability, and performance of dairy cows

“…The effects of feeding silage treated with LB on performance of dairy cows in the literature have been inconsistent. Although some studies have observed a greater milk yield response (Kung et al, 2003), others did not (Taylor et al, 2002;Arriola et al, 2011b;da Silva et al, 2017). Kleinschmit and Kung (2006) questioned whether the high acetate concentrations of LBinoculated forages would reduce DMI.…”

“…However, this has not been consistently supported in dairy cow studies. Several studies reported that LB did not affect intake (Taylor et al, 2002;Kung et al, 2003;Arriola et al, 2011b) or milk yield (Taylor et al, 2002;da Silva et al, 2017), whereas others reported an increase in milk yield (Kung et al, 2003;Ben-Meir et al, 2018).…”

Effects of Lactic Acid Bacteria Isolated From Rumen Fluid and Feces of Dairy Cows on Fermentation Quality, Microbial Community, and in vitro Digestibility of Alfalfa Silage

“…Currently, most of LAB inoculants for ensiling were isolated from plant-derived materials, including fresh forage, silage, and so on (Zhang et al, 2015(Zhang et al, , 2018Ni et al, 2017b;Wang et al, 2017). However, plant-derived LAB inoculants sometimes cannot improve animal performance (Kristensen et al, 2010;Santos et al, 2017;Silva et al, 2017). For example, Khota et al (2017) reported that although LAB from sweet corn stover silage could improve the silage quality, it did not show positive effect on in vitro dry matter (DM) digestibility and gas production (GP) of beef cattle.…”

Effect of Inoculation with Preactivated Lactobacillus Buchneri and Urea on Fermentative Profile, Aerobic Stability and Nutritive Value in Corn Silage

“…Corn (Zea mays L.) is a species that stands out for silage production because of its high nutritional value, suitable dry matter (DM) content, high concentration of fermentable carbohydrates, low buffering capacity and high digestibility [3]. However, corn silages, due to the high concentrations of water-soluble carbohydrates (WSC), afford a high content of lactic acid that causes a sharp drop in pH below 3.8, which allows the yeast's development that deteriorates the silage in a short time with exposure to air [4], and because these microorganisms are facultative anaerobic leaven, the residual soluble sugars and also yeasts are able to assimilate lactate, starting aerobic deterioration [3,[5][6][7]. According to [8], the corn plant may have 3 to 5 log cfu/g epiphytic yeasts and the low concentration of fermentation products with antifungal capacity compromises the silage's aerobic stability [9,10].…”