Effects of Nd:YAG laser on wound healing processes: Clinical and immunohistochemical findings in rat skin

Romanos, George E.; Pelekanos, Stavros; Strub, J. R.

doi:10.1002/lsm.1900160408

Cited by 46 publications

(24 citation statements)

References 21 publications

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“…Thus, laser is promising coadjutant treatment that can be used in the photodynamic therapy [12,13], stimulate cell differentiation, reduction of inflammation, and tissue repair [14][15][16][17]. Previous studies demonstrated that the specific parameters of low-level laser therapy LLLT can stimulate the deposition of collagen by fibroblasts [18] and proliferation of osteoblasts [19], as well as increase the mitochondrial activity and synthesis of ATP [20]. Once applied to cultured odontoblast-like cells, the LLLT increased cell metabolism as well as the synthesis of proteins and alkaline phosphate activity [14][15][16].…”

Section: Introductionmentioning

confidence: 99%

Effect of low-level laser therapy on odontoblast-like cells exposed to bleaching agent

et al. 2013

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The aim of the present study was to evaluate the effect of low-level laser therapy (LLLT) on odontoblast-like MDPC-23 cells exposed to carbamide peroxide (CP 0. 01 %-2.21 μg/mL of H 2 O 2 ). The cells were seeded in sterile 24-well plates for 72 h. Eight groups were established according to the exposure or not to the bleaching agents and the laser energy doses tested (0, 4, 10, and 15 J/cm 2 ). After exposing the cells to 0.01 % CP for 1 h, this bleaching solution was replaced by fresh culture medium. The cells were then irradiated (three sections) with a near-infrared diode laser (InGaAsP-780±3 nm, 40 mW), with intervals of 24 h. The 0.01 % CP solution caused statistically significant reductions in cell metabolism and alkaline phosphate (ALP) activity when compared with those of the groups not exposed to the bleaching agent. The LLLT did not modulate cell metabolism; however, the dose of 4 J/cm 2 increased the ALP activity. It was concluded that 0.01 % CP reduces the MDPC-23 cell metabolism and ALP activity. The LLLT in the parameters tested did not influence the cell metabolism of the cultured cells; nevertheless, the laser dose of 4 J/cm 2 increases the ALP activity in groups both with and without exposure to the bleaching agent.

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Section: Introductionmentioning

confidence: 99%

Effect of low-level laser therapy on odontoblast-like cells exposed to bleaching agent

et al. 2013

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“…It has been demonstrated that the photo-stimulation mediated by the LLLT, in addition to increase ATP synthesis [6], produces an analgesic effect [7][8][9] and stimulates cell division [10], accelerating tissue healing [11][12][13]. Some specific laser types also have the capacity to stimulate the synthesis and deposition of collagen matrix by fibroblasts [14][15][16], and the increase of mitochondrial activity and cell metabolism [8] and proliferation [12].…”

Section: Introductionmentioning

confidence: 99%

Nutritional stress enhances cell viability of odontoblastlike cells subjected to low level laser irradiation

Tagliani¹,

Oliveira²,

Lins³

et al. 2010

Laser Phys. Lett.

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In spite of knowing that cells under stress are biostimulated by low level laser (LLL) irradiation, the ideal condition of stress to different cell lines has not yet been established. Consequently, the aim of the present in vitro study was to evaluate the effects of a defined parameter of LLL irradiation applied on stressed odontoblast-like pulp cells (MDPC-23). The cells were seeded (12500 cells/cm 2 ) in wells of 24-well plates using complete culture medium (DMEM) and incubated for 24 hours. Then, the DMEM was replaced by a new medium with low concentrations (nutritional stress condition) of fetal bovine serum (FBS) giving rise to the following experimental groups: G1: 2% FBS; G2: 5% FBS; and G3: 10% FBS. The cells were irradiated three times with LLL in specific parameters (808±3 nm, 100 mW, 1.5 J/cm 2 ) every 24 hours. No irradiation was carried out in groups G4 (2% FBS-Control), G5 (5% FBS-Control), and G6 (10% FBS-Control). For all groups, the cell metabolism (MTT assay) and morphology (SEM) was evaluated. The experimental groups showed enhanced cell metabolism and normal cell morphology regardless of FBS concentration. A slight increase in the cell metabolism was observed only in group G2. It was concluded that cell nutritional stress caused by reducing the concentration of FBS to 5% is the most suitable method to assess the biostimulation of LLL irradiated MDPC-23 cells.MDPC-23 cells adhered to the glass substrate exhibited a spindle-shaped morphology and some mitosis were observed (arrows); SEM ×500

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“…[5][6][7] Some specific types of laser can also stimulate fibroblasts to synthesize and deposit collagen matrix. [8][9][10] A number of in vitro investigations using a wide range of cell cultures have provided different data concerning cell proliferation 11,12 and differentiation, 13 DNA and bone protein synthesis, 1,[14][15][16] and bone tissue formation without genotoxic or cytotoxic damage. 17 Unfortunately, these studies have particular irradiation setups and different methodologies, so that the results obtained cannot be compared with or extrapolated to others.…”

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confidence: 99%

A Novel 785-nm Laser Diode-Based System for Standardization of Cell Culture Irradiation

Lins

Oliveira

Guimarães

et al. 2013

Photomedicine and Laser Surgery

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Objective: The purpose of this study was to develop a novel device that concatenates alignment of infrared lasers and parallel procedure of irradiation. The purpose of this is to seek standardization of in vitro cell irradiation, which allows analysis and credible comparisons between outcomes of different experiments. Background data: Experimental data obtained from infrared laser therapies have been strongly dependent upon the irradiation setup. Although further optical alignment is difficult to achieve, in contact irradiation it usually occurs. Moreover, these methods eventually use laser in a serial procedure, extending the time to irradiate experimental samples. Methods: A LASERTable (LT) device was designed to provide similar infrared laser irradiation in 12 wells of a 24 well test plate. It irradiated each well by expanding the laser beam until it covers the well bottom, as occurs with unexpanded irradiation. To evaluate the effectiveness of this device, the spatial distribution of radiation was measured, and the heating of plain culture medium was monitored during the LT operation. The irradiation of LT (up to 25 J/cm 2 -20 mW/cm 2 ; 1.250 sec) was assessed on odontoblast-like cells adhered to the bottom of wells containing 1 mL of plain culture medium. Cell morphology and metabolism were also evaluated. Results: Irradiation with LT presented a Gaussian-like profile when the culture medium was not heated > 1°C. It was also observed that the LT made it 10 times faster to perform the experiment than did serial laser irradiation. In addition, the data of this study revealed that the odontoblast-like cells exposed to low-level laser therapy (LLLT) using the LT presented higher metabolism and normal morphology. Conclusions: The experimental LASERTable assessed in this study provided parameters for standardization of infrared cell irradiation, minimizing the time spent to irradiate all samples. Therefore, this device is a helpful tool that can be effectively used to evaluate experimental LLLT protocols.

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Effects of Nd:YAG laser on wound healing processes: Clinical and immunohistochemical findings in rat skin

Cited by 46 publications

References 21 publications

Effect of low-level laser therapy on odontoblast-like cells exposed to bleaching agent

Effect of low-level laser therapy on odontoblast-like cells exposed to bleaching agent

Nutritional stress enhances cell viability of odontoblastlike cells subjected to low level laser irradiation

A Novel 785-nm Laser Diode-Based System for Standardization of Cell Culture Irradiation

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