Effects of Silver Nitrate, Activated Charcoal and Cold Treatment on the in Vitro Androgenesis of Pepper (Capsicum Annuum L.)

Özkum, Dudu; Tıpırdamaz, Rukiye

doi:10.17660/actahortic.2007.729.20

Cited by 5 publications

(5 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…There are several factors affecting androgenesis in many species, such as genotypes (Mitykó et al, 1995;Rodeva et al, 2004), growth of donor plants, pre-treatments of anthers (Ö kum and Tripirdamaz, 2002;Koleva-Gudeva, 2003;Ashok Kumar et al, 2003), composition of medium Koleva-Gudeva and Spasenoski, 2001;Dolcet-Sanjuan et al, 1997) and the source of plant material (Kintzos et al, 2000). The response of anthers cultured on different media under certain thermal treatments can go in two directions, callus induction or embryo formation.…”

Section: Discussionmentioning

confidence: 99%

Somatic embryogenesis in pepper anther culture: The effect of incubation treatments and different media

Koleva-Gudeva¹,

Spasenoski²,

Trajkova³

2007

Scientia Horticulturae

View full text Add to dashboard Cite

The frequency of obtained androgenic plants depends highly on the genotype; therefore the low rate of haploid recovery limits the utility of anther culture in pepper breeding. The need for incubation treatment and adequate nutrition media supplemented with plant growth regulators, especially auxins, are suggested as important factors to obtain somatic haploid embryos in pepper anther culture. The effect of three incubation treatments of the androgenic potential in pepper anther culture on MS, N, LS, NN and CP medium are summarised, and the results demonstrate that:by incubating treatment in cold conditions (at 7 8C) in darkness for 7 days, and then transferring the explants to light conditions (12-h photoperiod at 25 8C) for 4 weeks, on LS and NN mediums, anthers produced callus; by incubating treatment in heat conditions (at 25 8C) in darkness for 7 days, and then transferring the explants to light conditions (12-h photoperiod at 25 8C) for 4 weeks, on MS and N mediums, anthers produced callus; by incubating treatment in heat conditions (at 35 8C) in darkness for 8 days, the next 4 days to light conditions (12-h photoperiod at 25 8C) on CP medium, and then transferring the explants to R 1 medium for 4 weeks, anthers produced embryos.

show abstract

Section: Discussionmentioning

confidence: 99%

Somatic embryogenesis in pepper anther culture: The effect of incubation treatments and different media

Koleva-Gudeva¹,

Spasenoski²,

Trajkova³

2007

Scientia Horticulturae

View full text Add to dashboard Cite

show abstract

“…Microspores in the process of first pollen mitosis occur in flower buds with corolla petals and calyx sepals of equal length or with petals slightly longer than sepals (Ö zkum and Tipirdamaz 2002;. Anthers are distinguished by the presence of a light anthocyan tinge in the distal end (Comlekcioglu et al 2001).…”

Section: Microspore Developmental Stagementioning

confidence: 99%

“…Supplements to the culture media Activated charcoal absorbs substances, including phenols and abscisic acid inhibitory to microspore embryogenesis from the agar and the environment of culture vessel as well as ingredients with negative effects produced by anther tissues (Kohlenbach and Wernicke 1978;Johansson 1983;Ö zkum and Tipirdamaz 2002). Activated charcoal can also absorb FeEDTA, pyridoxine, biotin, thiamine, folic acid, nicotine acid and hormone-supporting plant growth released from anther tissues (Weatherhead et al 1979;Johansson 1983;Johansson et al 1990;Vagera 1990).…”

Section: Plant Growth Regulatorsmentioning

confidence: 99%

“…Carbon stimulates embryogenesis in pepper and prolongs vitality and productivity of anthers by inhibiting callus proliferation, but inhibits growth of embryos and development of regenerants (Vagera and Havranek 1985;Boyaci 2001). Charcoal in media at concentrations from 0.25 to 2% (Morrison et al 1986b;Dolcet-Sanjuan and Claveira 1994;Ö zkum et al 2001;Ö zkum and Tipirdamaz 2002;Supena et al 2006), or in combination with carrot extract stimulates embryogenesis (Vagera 1984;Pandeva and Zagorska 1986;Pandeva et al 1990) (Table 1). Ellialtioglu et al (2001) observed increased embryo production on medium C and decreased on medium MS (Murashige and Skoog 1962) supplemented with carrot extract and activated charcoal.…”

Section: Plant Growth Regulatorsmentioning

confidence: 99%

See 1 more Smart Citation

Anther culture in pepper (Capsicum annuum L.) in vitro

2011

View full text Add to dashboard Cite

Pepper (Capsicum annuum L.) is an important vegetable crop that can be improved using plant tissue culture and biotechnology. However, it is difficult to develop appropriate breeding material by in vitro cultivation in this species. Haploid plant production is useful in the breeding programs to facilitate recovery of recessive mutations and unique genetic recombinations. In embryogenesis, haploid formation from pollen in anther culture is a scientifically advanced, but controversial system. Various techniques for haploid plant regeneration are used to establish an efficient double haploid production method. The purpose of this article is to summarize, through comparison, results in pepper anther culture, problems associated with work in this field, and the influence of critical factors for successful embryo formation and plantlet development.

show abstract

“…In these early studies, the regeneration of plants achieved was of low efficiency, and research efforts in the next experiments aimed to identify the factors influencing induction of androgenesis. After many experiments, it was found that androgenic response depended on the following: growing conditions, age and genotype of the donor plant (Ercan et al, 2006;Niklas-Nowak et al, 2012;Grozeva et al, 2013;KolevaGudeva et al, 2013;Alremi et al, 2014), developmental stage of microspores in the anther (Nowaczyk and Kisiała, 2006;Parra-Vega et al, 2013a;Barroso et al, 2015), composition of the culture medium, concentration and combination of growth regulators, organic and inorganic additives (Büyükalaca et al, 2004;Zhao et al, 2010;Taşkin et al, 2011;Roshany et al, 2013;Olszewska et al, 2014), and pretreatment of flower buds and/or anthers (KolevaGudeva et al, 2007;Özkum and Tıpırdamaz, 2007;Irikova et al, 2011b;Nowaczyk et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

Pepper (Capsicum annuum L.) anther culture: fundamentalresearch and practical applications

Irikova¹,

Kintzios²,

Grozeva³

et al. 2016

Turk J Biol

View full text Add to dashboard Cite

IntroductionIn general, haploid cells or individuals are those in which the original chromosome number has reduced by half. According to Seguí-Simarro (2010), androgenesis can be defined as a set of biological processes leading to an individual that genetically originated exclusively from a male nucleus. After spontaneous or induced genome doubling, double haploids (DHs) that are completely homozygous are obtained. The production of haploid plants can be induced by methods such as parthenogenesis, interspecific hybridization, pollination with irradiated pollen, hormonal and/or chemical treatment, and application of high-temperature stress (Forster et al., 2007;Murovec and Bohanec, 2013). In anther culture, haploids and DHs occur through direct microspore embryogenesis. This technique has no analogue for fast creation of pure homozygous lines in a single step. In pepper-plant breeding, for example, this method has helped in the development of important new varieties with improved adaptability, productivity, and disease and pest tolerance (Chunling and Baojun, 1995;Venczel and Mitykó, 1995;Arnedo Andrés et al., 2004;Nervo et al., 2007). DH lines are a suitable material for genetic and molecular studies, and this determines their high value in molecular breeding as well (Madhusudhana, 2015).Regarding the genus Capsicum, the first haploids were derived by in vitro anther culture of C. annuum and C.

show abstract

Effects of Silver Nitrate, Activated Charcoal and Cold Treatment on the in Vitro Androgenesis of Pepper (Capsicum Annuum L.)

Cited by 5 publications

References 0 publications

Somatic embryogenesis in pepper anther culture: The effect of incubation treatments and different media

Somatic embryogenesis in pepper anther culture: The effect of incubation treatments and different media

Anther culture in pepper (Capsicum annuum L.) in vitro

Pepper (Capsicum annuum L.) anther culture: fundamentalresearch and practical applications

Contact Info

Product

Resources

About