Effects of tetramethylpyrazine on nitric oxide/cGMP signaling after cerebral vasospasm in rabbits

Shao, Zhengkai; Li, Jingwen; Zhao, Zhenqun; Cheng, Gao; Sun, Zhe; Liu, Xiangzhen

doi:10.1016/j.brainres.2010.09.011

Cited by 28 publications

(16 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…External supplementation of NO was shown to prevent or reverse CV in many experimental studies. 28,29 A new generation of NO donors has been in the focus of recent research to avoid the drawbacks of classic drugs. Different NO donors have been tested with regard to their potential role in treating CV.…”

Section: Discussionmentioning

confidence: 99%

Effect of Visible Light on Vasospasticity of Post–Subarachnoid Hemorrhage Cerebrospinal Fluid

Sabancı

Omay

Aras

et al. 2017

J Neurol Surg A Cent Eur Neurosurg

View full text Add to dashboard Cite

Cerebral vasospasm (CV) is a serious complication of subarachnoid hemorrhage (SAH) with high morbidity and mortality rates. The mechanism of CV has not been determined. There are many theories related to this unsolved issue, one of which supports CV as a two-stage phenomenon from a pathophysiologic perspective. The first stage consists of inhibition of neuronal nitric oxide synthase by oxyhemoglobin, which results in a decrease of nitric oxide (NO) production. The second stage consists of an increase in the levels of asymmetric dimethylarginine through bilirubin oxidation products (BOXes), which are oxidized by-products of hemoglobin metabolism. These in turn inhibit endothelial nitric oxide synthase (eNOS), which results in the blockage of the second NO production mechanism. BOXes are sensitive to visible light, as is their precursor bilirubin. The hypothesis of CV prevention using the photosensitivity of BOXes was tested in this study. Cerebrospinal fluid (CSF) obtained from two patients with SAH was divided in half and either exposed to a standard dose of visible light or not exposed to any light. The CSF was spectrophotometrically investigated and the concentration of BOXes was measured. A comparison between CSF samples exposed to light and not exposed to light was made. Using two groups of 16 rats each, the vasospastic effect of the CSF exposed and not exposed to light on arteries of the cortical surface was measured. The cortex was exposed using the cranial window. Spectrophotometric analysis revealed that the concentration of BOXes in the CSF decreased significantly after being exposed to visible light ( < 0.001). There was a significant difference of the vasospastic effect of CSF on exposed cortical arteries ( < 0.001). The concentration of BOXes and the vasospastic effect of CSF taken from patients with SAH were significantly reduced after being exposed to visible light if compared with CSF not exposed to light.

show abstract

Section: Discussionmentioning

confidence: 99%

Effect of Visible Light on Vasospasticity of Post–Subarachnoid Hemorrhage Cerebrospinal Fluid

Sabancı

Omay

Aras

et al. 2017

J Neurol Surg A Cent Eur Neurosurg

View full text Add to dashboard Cite

show abstract

“…It has been suggested that EBI may exacerbate, maintain and even trigger DCVS 12 , and as such this model may aid in investigating both the early and late DCVS phases, including EBI and DCVS interactions following SAH. In particular, repeatable in vivo DCVS monitoring techniques including DSA 13 , computed tomography angiography 14 , and transcranial Doppler 15 are more readibly applied in rabbits than in smaller laboratory animals. In addition to enabling examiner-independent spontaneous SAH, the model allows the user to control for different degrees of severity (amount of blood or ICP, and consequent changes in cerebral perfusion pressure).…”

Section: Discussionmentioning

confidence: 99%

The Rabbit Blood-shunt Model for the Study of Acute and Late Sequelae of Subarachnoid Hemorrhage: Technical Aspects

Andereggen

Neuschmelting

Gunten³

et al. 2014

JoVE

View full text Add to dashboard Cite

Early brain injury and delayed cerebral vasospasm both contribute to unfavorable outcomes after subarachnoid hemorrhage (SAH). Reproducible and controllable animal models that simulate both conditions are presently uncommon. Therefore, new models are needed in order to mimic human pathophysiological conditions resulting from SAH. This report describes the technical nuances of a rabbit blood-shunt SAH model that enables control of intracerebral pressure (ICP). An extracorporeal shunt is placed between the arterial system and the subarachnoid space, which enables examiner-independent SAH in a closed cranium. Step-by-step procedural instructions and necessary equipment are described, as well as technical considerations to produce the model with minimal mortality and morbidity. Important details required for successful surgical creation of this robust, simple and consistent ICP-controlled SAH rabbit model are described.

show abstract

“…Like the parental herb, TMP (also called ligustrazine) is widely used to treat vascular diseases in China, with few adverse reactions in humans [11]. TMP has a long history of use, and the latest studies report its antioxidant proficiency in animal models of ischemic reperfusion [12], atherosclerosis [13], and cerebral vasospasm [14]. However, the underlying mechanism of TMP to combat detrimental ROS production and to rescue endothelial dysfunction in artery walls remains unclear.…”

Section: Introductionmentioning

confidence: 99%

Tetramethylpyrazine Protects against Hydrogen Peroxide‐Provoked Endothelial Dysfunction in Isolated Rat Aortic Rings: Implications for Antioxidant Therapy of Vascular Diseases

Wong

et al. 2014

Evidence-Based Complementary and Alternative Medicine

View full text Add to dashboard Cite

Background and Objectives. Oxidative stress can initiate endothelial dysfunction and atherosclerosis. This study evaluated whether tetramethylpyrazine (TMP), the predominant active ingredient in Rhizoma Ligustici Wallichii (chuanxiong), prevents endothelial dysfunction in a rat model of oxidative stress. Methods. Isolated rat aortic rings were pretreated with various drugs before the induction of endothelial dysfunction by hydrogen peroxide (H2O2). Changes in isometric tension were then measured in acetylcholine- (ACh-) relaxed rings. Endothelial nitric oxide synthase (eNOS) expression was evaluated in the rings by Western blotting, and superoxide anion (O2 ∙−) content was assessed in primary rat aortic endothelial cells by dihydroethidium- (DHE-) mediated fluorescence microscopy. Results. ACh-induced endothelium-dependent relaxation (EDR) was disrupted by H2O2 in endothelium-intact aortic rings. H2O2-impaired relaxation was ameliorated by acute pretreatment with low concentrations of TMP, as well as by pretreatment with catalase and the NADPH oxidase inhibitors, apocynin and diphenyleneiodonium (DPI). TMP, apocynin, and DPI also reduced O2 ∙− accumulation in endothelial cells,but TMP failed to alter eNOS expression in aortic rings incubated with H2O2. Conclusions. TMP safeguards against oxidative stress-induced endothelial dysfunction, suggesting that the agent might find therapeutic utility in the management of vascular diseases. However, TMP's role in inhibiting NADPH oxidase and its vascular-protective mechanism of action requires further investigation.

show abstract

Effects of tetramethylpyrazine on nitric oxide/cGMP signaling after cerebral vasospasm in rabbits

Cited by 28 publications

References 38 publications

Effect of Visible Light on Vasospasticity of Post–Subarachnoid Hemorrhage Cerebrospinal Fluid

Effect of Visible Light on Vasospasticity of Post–Subarachnoid Hemorrhage Cerebrospinal Fluid

The Rabbit Blood-shunt Model for the Study of Acute and Late Sequelae of Subarachnoid Hemorrhage: Technical Aspects

Tetramethylpyrazine Protects against Hydrogen Peroxide‐Provoked Endothelial Dysfunction in Isolated Rat Aortic Rings: Implications for Antioxidant Therapy of Vascular Diseases

Contact Info

Product

Resources

About