More than one method has been developed to increase flexibility. One of these methods is foam rolling applications. Although they have different forms, vibrating foam rollers have different frequencies. This study aims to determine the effects of foam roller application applied at different frequency levels on flexibility. Twenty male amputee athletes participating in the study were randomly divided into two groups. Then the flexibility levels of the players were evaluated. After the measurement, vibrating foam rollers with a frequency of 32 Hz and 62 Hz were applied to the 1st and 2nd groups, respectively. Finally, the flexibility measurement was repeated. For the data analysis, 2x2 repeated measures ANOVA was used. As a result of the analyses performed to determine the changes in the range of motion, the main time effect was found to be significant (p <0.001), while the time and group interaction were not significant (p= 0.75). Post Hoc analysis showed that the foam roller application with both frequencies was significant (p <0.001) in increasing range of motion. However, frequency differences do not affect flexibility levels (p= 0.52). The result of this study indicates that the application of vibration foam rolling at different frequency levels is an effective method to increase range of motion. It is also seen that increasing the frequency level does not affect flexibility.