Effects of the food contaminant semicarbazide following oral administration in juvenile Sprague–Dawley rats

Maranghi, Francesca; Tassinari, Roberta; Lagatta, Vincenzo; Moracci, Gabriele; Macrı̀, Caterina; Eusepi, Agostino; Virgilio, Antonio Di; Scattoni, María Luisa; Calamandrei, Gemma

doi:10.1016/j.fct.2008.12.003

Cited by 52 publications

(43 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In view of these toxicological effects of chronic exposure to semicarbazide, the no observed adverse effect level (NOAEL) was estimated by the authors to be lower than 18–21 mg/kg/day in rat. Accordingly, the NOAEL in juvenile rats on another strain has recently been proposed to be lower than 40 mg/kg for semicarbazide oral administration [21]. In this study describing the toxicity of semicarbazide on biochemical and behavioural parameters, the authors observed a clear-cut inhibition of body weight gain and defects in cartilage mineralization [21].…”

Section: Discussionmentioning

confidence: 99%

“…Moreover, the prevention of body weight gain observed during oral administration of semicarbazide was not accompanied by any worsening of the plasma levels of metabolites or oxidative stress markers. Finally, since semicarbazide oral toxicity has been recently suspected [21–23], the need for further investigations of the putative antiobesity effects of other SSAO inhibitors is discussed. …”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Oral Administration of Semicarbazide Limits Weight Gain together with Inhibition of Fat Deposition and of Primary Amine Oxidase Activity in Adipose Tissue

Mercader

Iffiú-Soltész²,

Bour³

et al. 2011

Journal of Obesity

View full text Add to dashboard Cite

An enzyme hitherto named semicarbazide-sensitive amine oxidase (SSAO), involved in the oxidation of primary amines, is abundantly expressed in adipocytes. Although SSAO physiological functions remain unclear, several molecules inhibiting its activity have been described to limit fat accumulation in preadipocyte cultures or to reduce body weight gain in obese rodents. Here, we studied whether oral administration of semicarbazide, a prototypical SSAO inhibitor, limits fat deposition in mice. Prolonged treatment with semicarbazide at 0.125% in drinking water limited food and water consumption, hampered weight gain, and deeply impaired fat deposition. The adiposomatic index was reduced by 31%, while body mass was reduced by 15%. Such treatment completely inhibited SSAO, but did not alter MAO activity in white adipose tissue. Consequently, the insulin-like action of the SSAO substrate benzylamine on glucose transport was abolished in adipocytes from semicarbazide-drinking mice, while their insulin sensitivity was not altered. Although semicarbazide is currently considered as a food contaminant with deleterious effects, the SSAO inhibition it induces appears as a novel concept to modulate adipose tissue development, which is promising for antiobesity drug discovery.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Oral Administration of Semicarbazide Limits Weight Gain together with Inhibition of Fat Deposition and of Primary Amine Oxidase Activity in Adipose Tissue

Mercader

Iffiú-Soltész²,

Bour³

et al. 2011

Journal of Obesity

View full text Add to dashboard Cite

show abstract

“…Reduction of staining intensity of the cartilage matrix is considered to reflect the decreased polymerization of cartilage matrix or absence of mineralization (Ramamurti and Taylor 1959;Maranghi et al 2009), indicating alteration of matrix properties caused by inhibition of cross-linking reactions of collagen. Because fissures specifically occurred in the sites subject to body weight loading such as the anterior area of the TABLE 3.-Histopathological findings for the bones and joints in young and adult rats.…”

Section: Discussionmentioning

confidence: 99%

Age-related Susceptibility to Induction of Osteochondral and Vascular Lesions by Semicarbazide Hydrochloride in Rats

Takahashi

Yoshida²,

Inoue³

et al. 2010

Toxicol Pathol

View full text Add to dashboard Cite

To compare the susceptibility to toxicity of semicarbazide hydrochloride (SEM-HCl) between young and adult rats, 3-and 20-week-old female SD rats were given a diet containing SEM-HCl at 0, 500, or 1,000 ppm and 0 or 1,000 ppm, respectively, for 4 weeks. Half of the animals were then maintained on basal diet for a further 2 weeks as recovery groups. Only in young rats was deformation of the knee joints as well as thorax and tail observed at 500 and 1,000 ppm. Histopathologically, severe osteochondral lesions, such as disarrangement and thickening of the epiphyseal cartilage and deformation of articular cartilage, were observed, but the severity of these lesions became reduced during the recovery period. In adult rats, osteochondral lesions were relatively mild. Fissures in the cartilage matrix of the tibia were characteristic of adult rats, and in these, reduction of severity was not obvious in the recovery group. In the thoracic aorta, the appearance of elastic laminae was altered only in young rats in both the 4-week treatment and recovery groups. These results suggest that growing animals are more susceptible to toxicity of SEM-HCl than adults are. Effects and the induced lesions link to the developing stage of the target organs.

show abstract

“…SEM is a kind of compound being carcinogenic to humans similar to the nitrofurazone (Vlastos et al 2010). By experiments about the oral SEM in rats, F. Maranghi found that the growth rate of the food intake and weight of male rats and female juvenile rats decreased significantly; besides, moderate and high doses of SEM would change the weight of the uterus, delay pubertal development, and even damage the spleen tissue and thyroid gland (Maranghi et al 2009). As a consequence, the Chinese National Standards (GB2760-2011) stipulated that the dose of ADC must not be higher than 45 mg/kg and many European countries had already ban the use of ADC in flour.…”

Section: Introductionmentioning

confidence: 97%

Prediction of Azodicarbonamide in Flour Using Near-Infrared Spectroscopy Technique

et al. 2016

View full text Add to dashboard Cite

Abstracts Azodicarbonamide is wildly used as a flour gluten fortifier in many countries, but according to the research results of toxicology of azodicarbonamide, its acute toxicity is slightly toxic. A dosage of 10 g/kg is lethal to mice, and it was proved by some researches to be dangerous or unhealthy for people and not suitable to be added in flour; hence, there is a need to identify the concentration of azodicarbonamide in flour quickly. Compared to traditional methods like highperformance liquid chromatography, the core advantage of near-infrared reflectance spectroscopy is rapid and economical. Spectral data in a range of 850 to 1050 nm were obtained by scanning 101 samples with different concentrations. The Mahalanobis distance method was used to distinguish abnormal spectral data, and the correlation coefficient method was used to choose characteristic wave bands. Radial basis function in combination with near-infrared reflectance spectroscopy was used to establish models in accordance. The limit of quantitation and the limit of detection of the first model were 72 and 15 mg/kg, respectively. Through analyzing the relative tolerances of predictive values and true values, the method of secondary modeling was proposed for low-concentration (72 mg/kg) samples. The predictions showed that nearinfrared reflectance spectroscopy could be used for detecting the content of azodicarbonamide added to flour.

show abstract

Effects of the food contaminant semicarbazide following oral administration in juvenile Sprague–Dawley rats

Cited by 52 publications

References 29 publications

Oral Administration of Semicarbazide Limits Weight Gain together with Inhibition of Fat Deposition and of Primary Amine Oxidase Activity in Adipose Tissue

Oral Administration of Semicarbazide Limits Weight Gain together with Inhibition of Fat Deposition and of Primary Amine Oxidase Activity in Adipose Tissue

Age-related Susceptibility to Induction of Osteochondral and Vascular Lesions by Semicarbazide Hydrochloride in Rats

Prediction of Azodicarbonamide in Flour Using Near-Infrared Spectroscopy Technique

Contact Info

Product

Resources

About