Effects of the preservation medium and storage duration of domestic cat ovaries on the maturational and developmental competence of oocytes &lt;i&gt;in vitro&lt;/i&gt;

Yoshida, Takumi; Alam, Md. Emtiaj; Hanafusa, Keisuke; Tsujimoto, Yuki; Tsukamoto, Manabu; Kanegi, Ryoji; Inaba, Toshio; Sugiura, Kikuya; Hatoya, Shingo

doi:10.1262/jrd.2021-084

Cited by 6 publications

(4 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Numerous studies have revealed that adding sugar to an extender positively impacts the motility, vitality, and plasma membrane integrity (PMI) of mammalian semen after cryopreservation [ 6 – 9 ]. Various studies used fructose or glucose in a tris-citric acid diluent to freeze mammalian sperm [ 10 – 14 ].…”

Section: Introductionmentioning

confidence: 99%

Effects of glucose and trehalose on tris-citric acid-egg yolk-fructose diluents for semen cryopreservation in goat

Kamal¹,

Alam²,

Das³

et al. 2023

J Adv Vet Anim Res

View full text Add to dashboard Cite

Objectives: This study aimed to examine the impacts of the wide range of concentrations of glu¬cose and trehalose on the tris-citric acid-egg yolk-fructose (TCEF) extenders for cryopreservation of goat semen. Materials and Methods: The sperm sample was pooled, washed, and diluted in control (TCEF without glucose and trehalose), TCEF + glucose (75, 150, 450, and 900 mm), and TCEF + trehalose (75, 150, 450, and 900 mm). After equilibrations, the semen straws were frozen under LN2 in the LN2 tank. After LN2 storage, the straws were thawed at 37°C for 30 seconds. The sperm parame¬ters of all study groups were checked after equilibration and freezing. Results: After equilibration, the progressive motility (PM), total motility (TM), and viability of sperm in G-75, G-150, G-450, T-75, T-150, and T-450 were not significantly different (p < 0.05) from those in control. After cryopreservation and thawing, the PM, TM, and plasma membrane integrity (PMI) of T-150 were significantly higher (p < 0.05) than in control, G-75, G-900, T-75, and T-900. The viability of sperm in T-150 was substantially higher (p < 0.05) than in the control, whereas there was no significant difference among the control, G-75, G-900, T-75, and T-900. However, the acrosome integrity (AI) of sperm in G-900 was significantly decreased (p < 0.05) compared to the control, G-75, G-150, G-450, T-75, T-150, and T-450. Conclusion: According to the findings, the supplementation of 150 mm trehalose in the TCEF dilu¬ent was more efficient for sperm cryopreservation in the buck as reflected by PM, TM, viability, PMI, and AI.

show abstract

Section: Introductionmentioning

confidence: 99%

Effects of glucose and trehalose on tris-citric acid-egg yolk-fructose diluents for semen cryopreservation in goat

Kamal¹,

Alam²,

Das³

et al. 2023

J Adv Vet Anim Res

View full text Add to dashboard Cite

show abstract

“…During freezing, the sperm are exposed to a wide range of temperatures, which causes structural and functional changes. So, for long-term sperm preservation until artificial insemination, the spermatozoa need an extender containing appropriate cryoprotectants, a sufficient quantity of nutrients, a well-buffered agent like tris, and a cold shock protector like egg yolk during the storage in liquid nitrogen [ 9 , 25 – 31 ]. Moreover, cryopreservation still causes semen damage in various animals [ 32 , 33 ], and researchers are still determining the optimum ingredient levels of the semen extender for freezing buck semen.…”

Section: Discussionmentioning

confidence: 99%

“…During semen cryopreservation, any changes in pH could affect the metabolism rates, motility, viability, and plasma membrane integrity [ 34 , 35 ]. Sodium citrate, HEPES, bicarbonate, and tris have been used as pH controllers to maintain the pH in the extender [ 9 , 19 , 25 ]. The pH of tris is representative of most living cells.…”

Section: Discussionmentioning

confidence: 99%

Effects of tris (hydroxymethyl) aminomethane and egg yolk on the cryopreservation of buck semen

et al. 2022

View full text Add to dashboard Cite

Objectives: This study was designed to examine the effects of various concentrations of tris (hydroxymethyl) aminomethane (tris) and egg yolk on the quality of cryopreserved buck sperm. Materials and Methods: The collected semen samples were pooled, washed, and diluted into five different freezing extender groups, viz., extender I (tris 0% + egg yolk 0%), extender II (tris 1.41% + egg yolk 4%), extender III (tris 2.41% + egg yolk 8%), extender IV (tris 3.41% + egg yolk 16%), and extender V (tris 4.41% + egg yolk 24%). The sperm parameter of the five groups of extenders was evaluated after equilibration and cryopreservation. Results: The results showed that extenders II–V provided significantly higher semen progressive motility and total motility percentages than extender I after equilibration (p < 0.05). The higher percentages of semen progressive motility, total motility, viability, and plasma membrane integ¬rity (by both HOST under light microscopy and stain after HOST under light microscopy) were found in the sperm cryopreserved with extender IV than extender I, extender II, and extender III groups after thawing (p < 0.05). In addition, semen progressive motility, total motility, and viability were not further increased, or plasma membrane integrity (by both HOST tests) was decreased by the addition of tris and egg yolk (extender V) after cryopreservation (p < 0.05). Conclusion: In conclusion, our result indicates that the following washing, the supplementation of tris (3.41% + egg yolk 16%) on the freezing extender are suitable for improving the semen quality of buck after freezing and thawing.

show abstract

“…O uso dos oócitos de espécies domésticas para o entendimento da maturação in vitro de gametas contribui para o conhecimento e melhoria das técnicas de reprodução assistida em fêmeas ruminantes (EL-SOKARY et al, 2022;MASTROROCCO et al, 2021), conservação de animais em extinção como as espécies carnívoras, as quais utilizam ovários de fêmeas caninas e felinas domésticas como modelo (YOSHIDA et al, 2022;VIARIS DE LESEGNO, et al, 2008).…”

Section: Introductionunclassified

Maturação in vitro de complexos cumulus oócitos caninos e bovinos

Petry,

Crippa,

Boni

et al. 2023

VIECIT

View full text Add to dashboard Cite

Maturação oocitária é uma das etapas da produção in vitro de embriões (PIVE) nas espécies domésticas, na qual as fêmeas bovinas e caninas são submetidas a diferentes métodos para obtenção de complexos cumulus oócitos (CCOs), in vivo e ou in vitro. Após a aquisição desses CCOs, os mesmos são rastreados e avaliados inicialmente quanto às características morfológicas, podendo ser submetidos à testes mais precisos, principalmente no desenvolvimento de pesquisa. Neste sentido, marcadores de viabilidade no decorrer da maturação e ao término da mesma podem ser utilizados. Esta etapa executada com alto controle de qualidade visa índices de maturação compatíveis e satisfatórios aos processos de fertilização in vitro (FIV), os quais consistem no próximo passo para PIVE. A reprodução assistida (RA) nas espécies abordadas apresenta objetivos comuns quanto à maturação oocitária, no entanto, podem ser destinas a fins diferentes quanto a aplicabilidade das técnicas e preservação do material genético. Nos cães, utilizados como modelo para PIVE de canídeos em extinção, o processo de maturação além de fisiologicamente distinto das demais espécies domésticas, tem um período de 72 horas, o que faz desta espécie um grande desafio nas taxas de maturação oocitária e subsequente PIVE quando comparadas com os bovinos.

show abstract

Effects of the preservation medium and storage duration of domestic cat ovaries on the maturational and developmental competence of oocytes <i>in vitro</i>

Cited by 6 publications

References 22 publications

Effects of glucose and trehalose on tris-citric acid-egg yolk-fructose diluents for semen cryopreservation in goat

Effects of glucose and trehalose on tris-citric acid-egg yolk-fructose diluents for semen cryopreservation in goat

Effects of tris (hydroxymethyl) aminomethane and egg yolk on the cryopreservation of buck semen

Maturação in vitro de complexos cumulus oócitos caninos e bovinos

Contact Info

Product

Resources

About