Effects of Vitamin E and Coenzyme Q10 Supplementation Against Doxorubicin-Induced Neurotoxicity in Rats

Abstract

Objective: This work was designed to investigate the effect of Vitamin E and the coenzyme Q10 (CoQ10) supplementation on neurotoxicity induced by doxorubicin (DOX) in rats.Methods: Forty-nine adult Albino rats of both sexes were utilized in this study; animals were randomly enrolled into seven groups of seven animals each. Group I: Control (rats administered corn oil); Group II: Vitamin E at a dose of 100 mg/kg/day for 3 weeks; Group III: CoQ10 at a dose of 50 mg/kg/day for 3 weeks; Group IV: DOX (2.5 mg/kg) intraperitoneally (IP) injected every other day for 2 weeks; Group V: Vitamin E (100 mg/kg/day) orally administered for 3 weeks prior to a DOX 2.5 mg/kg IP injected every other day for 2 weeks; Group VI: CoQ10 (50 mg/kg/day) for 3 weeks orally administered prior to an IP dose of DOX 2.5 mg/kg every other day for 2 weeks; and Group VII: CoQ10 (50 mg/kg/day), Vitamin E (100 mg/kg) for 3 weeks orally administered prior to an IP dose of DOX 2.5 mg/kg every other day for 2 weeks. Twenty-four hour after the end of the treatment duration, brain of each animal was excised and part of it to be utilized to prepare homogenate for the estimation of caspase-3 (CASP-3), and the remaining part is used for immunohistochemistry examination and to estimate the percent of apoptotic index by terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick end labeling (TUNEL) assay.Results: Vitamin E and CoQ10 significantly (p<0.05) reduced CASP-3, reduced the percent apoptotic index of TUNEL-assay, and there was an improvement in the immunohistochemistry of rats’ brain in Groups V, Group VI, and group VII by reducing number of apoptotic cells compared to Group IV.Conclusion: Both Vitamin E and CoQ10 may have a protective effect against DOX-induced neurotoxicity in rats.

Metoprolol-Induced Liver Injury and the Hepatoprotective Role of Vitamin E in Rabbits

“…The animals were housed in the animal house at Basra College of Pharmacy; they were kept in stainless steel cages for acclimatization, with free access to food and water and a 12:12-h cycle of light and darkness. The animals were not fed for Zubairi 12 hours before the experiment. The study was approved by the Local Institutional Ethical Committee of the Faculty of Pharmacy College at the University of Basra.…”

“…In addition, this critical vitamin has a defensive role in that it minimizes the harmful effects of oxidation that causes damage to the liver and other body parts [11]. Furthermore, Vitamin E has a notably positive effect on cell membranes as it has the effect of protecting the polyunsaturated fats within them, therefore, preserving the structure and function of the membranes [12].…”

Doxorubicin-Induced Cardiotoxicity in Adult Male Albino Rats and the Ameliorative Effects of Achillea Fragrantissima Crude Extract, Its Combined Ethyl Acetate/n. Butanol Fraction, and Vitamin E

“…Polyunsaturated fats in cell membranes, which are critical for membrane structure and function, can also be protected by vitamin E (15). Additional benefits of vitamin E include maintaining healthy red blood cells, promoting better circulation and supporting healthy muscle and nervous system function (16).…”