Efficacy of antibiotic sensitivity and antimicrobial activity of Streptomyces cinereoruber RSA-14 isolated from rhizosphere soil of Alternanthera sessilis (L.) R. Br. ex DC

Abstract

The search for novel antibiotics is of immense importance in research areas around the world for agricultural, pharmaceutical, and industrial applications. The Streptomyces species are widely used as an important biological tool for the production of a wide range of novel secondary metabolites. In the present study, isolated strain RSA-14 from rhizosphere soil of Alternanthera sessilis was subjected to morphological, physiological, biochemical and 16S rRNA gene sequence analysis. The isolated RSA-14 was analyzed for antimicrobial activities by cross streak method and exhibited a broad spectrum of antimicrobial activity against test pathogens. The isolate was tested for the ability to grow in the presence of antibiotics, such as penicillin, streptomycin, chloramphenicol, gentamycin, and tetracycline and resistant to only two antibiotics, and sensitive to others. The 16S ribosomal RNA gene sequencing and analysis of the phylogenetic tree showed 100% sequence similarity with Streptomyces cinereoruber strain P.B.373.

Antimicrobial Efficacy of 7-Hydroxyflavone Derived from Amycolatopsis sp. HSN-02 and Its Biocontrol Potential on Cercospora Leaf Spot Disease in Tomato Plants

“…BLAST analysis was performed to ascertain the phylogenetic neighbors of the isolate using the EzBioCloud platform (https://www.ezbiocloud.net, accessed on 6 April 2023). Finally, an evolutionary tree was constructed with similar type strains using MEGA7.0 software [56].…”

“…Sterile distilled water was employed as a negative control. The plates were incubated for 24 h at 37 • C. The antimicrobial assay was repeated thrice, and the mean zone of inhibition was calculated for each pathogen [56].…”