Elastase Activity Inhibition by the Most Active Fraction of Star Fruit (Averrhoa Carambola L.) Leaves From Three West Java Regions

Mardhiyah, Siti; Elya, Berna; Noviani, Arikadia

doi:10.22159/ijap.2020.v12s1.ff020

Cited by 3 publications

(4 citation statements)

References 17 publications

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“…Moreover, polyphenols in a sample will interact with the amino acids of the enzyme such as serine, histamine, and aspartate residues through hydrogen bonds, causing a decrease in catalytic activity and denaturation of the enzyme (Brás et al, 2010;Mardhiyah et al, 2020). The presence of flavonoid polyphenols in the extract and fractions of the M. calabura allows for a role in providing anti-elastase activity.…”

Section: Resultsmentioning

confidence: 99%

In vitro anti-aging activity of Muntingia calabura L. fruit extract and its fractions

Nur¹,

Angelina²,

Aswad³

et al. 2021

J Pharm Pharmacogn Res

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Context: Premature aging usually occurred due to free radicals reducing the skins’ physiological functions. Muntingia calabura, a plant containing rich antioxidants, has the potential to overcome this problem. Aims: To evaluate the antioxidant capacity of M. calabura in inhibiting the premature aging process, to be potentially developed into an antiaging active ingredient. Methods: The samples were extracted using ethanol 96%, and processed into n-hexane, ethyl acetate, and ethanol fractions, respectively. Total phenolic content was determined, followed by the evaluation of antioxidant capacity through DPPH, FRAP, and ABTS assay. Further, anti-elastase was conducted using human neutrophil elastase as a skin degradation enzyme, followed by an anti-collagenase test. Finally, normal cell proliferation was also evaluated via the MTT method measuring cell viability on HDFa cells. Results: As the results, ethanol extract, ethyl acetate fraction, and ethanol fraction showed a strong antioxidant effect, having great capacity reducing DPPH, ABTS radicals, and also iron reduction, in contrast to n-hexane fraction that exhibited only weak activity. The antioxidant trend capacities were found directly correlated to total phenolic contents. Furthermore, the ethyl acetate fraction was found to have optimum activity in inhibiting elastase and collagenase enzymes, showing a similar impact on cell viability. Conclusions: The ethyl acetate fraction from M. calabura exhibits the prospect for further development to support its effectiveness as an active ingredient in antiaging cosmetics.

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Section: Resultsmentioning

confidence: 99%

In vitro anti-aging activity of Muntingia calabura L. fruit extract and its fractions

Nur¹,

Angelina²,

Aswad³

et al. 2021

J Pharm Pharmacogn Res

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“…The DPPH radical scavenging activity (DRSA) was examined using the modified microplate method [22,23]. DPPH was dissolved in methanol (6 mg/100 ml), and 180 µl was added to extracts 20 µl (final conc.…”

Section: Determination Of Anti-oxidantmentioning

confidence: 99%

Unripe Fruit of Rubus Fraxinifolius as a Potential Source of Antioxidant and Antielastase Agent

Desmiaty¹,

Elya²

2021

Int J App Pharm

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Objective: This research aimed to examine the anti-oxidant activity, antielastase activity, and the content of total phenolic and total flavonoid of R. fraxinifolius unripe fruit. Methods: The dried unripe fruit was extracted using Soxhlet apparatus with sequence solvent: n-hexane, ethyl acetate, and methanol. Each extract was determined the anti-oxidant and antielastase activity, total phenolic, and total flavonoid content. Result: The result showed the extracts (n-hexane, ethyl acetate, and methanol) gave anti-oxidant IC50>200; 186.84; and 19.74 ppm, and the ability of elastase inhibition was 6.84+0.9%; 52.23+7.1%; and 57.81+5.5% at 100 ppm, respectively. The methanolic extract contained phenolic 202.2 mg GAE/g extract and flavonoid 43.89 mg QE/g extract. Conclusion: R. fraxinifolius unripe fruit has shown potential as a DPPH (α, α-diphenyl-β-picrylhydrazyl) radical scavenger and anti-elastase. This study provides an excellent effect to underline the importance of R. fraxinifolius unripe fruit, and it can be developed as nutricosmetics, nutraceuticals, or herbal anti-wrinkle cosmetics.

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“…21 Research conducted by Mardhiyah et al, 2020 reported that the water fraction of A. carambola Depok leaves with a total polyphenol and flavonoid content of 115.68 mg gallic acid equivalent g extract and 9.15 mg equivalent quercetin / g extract can inhibit proteolytic activity elastase and prevent premature skin aging. 2 Based on the result on this research on elastase inhibitory activity, total flavonoid and total polyphenol content, it is considered that Faloak stem bark extract can be considered as potential to be developed as active compound in anti-aging product, both in cosmetic and pharmaceutical dosage forms.…”

Section: Determination Of Total Flavonoids and Total Polyphenolmentioning

confidence: 99%

“…The higher the total polyphenol content and total flavonoid levels, the higher the activity inhibition against elastase. 2 Elastase is an enzyme that is responsible for the breakdown of elastin. 3 Misregulation of the elastase enzyme is involved in the skin aging process.…”

Section: Introductionmentioning

confidence: 99%

Elastase Inhibitory Activity, Determination of Total Polyphenol and Determination of Total Flavonoids and Pharmacognosy Study of Faloak Plant (Sterculia quadrifida R.Br) from East Nusa Tenggara-Indonesia

Radjah

Putri²,

Elya³

2021

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Introduction: Faloak (Sterculia quadrifida R. Br) is one of the typical plants of East Nusa Tenggara (NTT). Faloak contain flavonoid and polyphenol compounds, and show strong antioxidants activity which potentially correlated with its elastase inhibitory activity. Therefore, in this research, elastase inhibitory activity on various part of Faloak plant was investigated. Objective: The purpose of this research was to investigate the elastase inhibitory activity, determination of total polyphenol, determination of total flavonoids, and also pharmacognosy characterization of Faloak leaves, roots, stems and stem barks. Methods: Sample of leaves, roots, stems, and stem barks were extracted by 70% ethanol using ultrasound-assisted extraction (UAE). Phytochemical screening, microscopic identification and elastase inhibitory activity testing were performed on the leaves, roots, stems, and stem barks extract. This extract with the highest elastase inhibitory activity was then determined for its total polyphenol content and of total flavonoids content. Results: UAE method with 70% ethanol successfully extracted active compounds from leaves, stems, roots, and stem barks of Faloak. Extract of all Faloak parts contained alkaloids, flavonoids, tannins, terpenes, and glycosides. The extract of Faloak stem barks showed the strongest elastase inhibitory activity as compared to the extract from other parts, with IC50 of 73.7 µg/mL. Alkaloid, flavonoid, tannin, terpene, and glycoside were detected as secondary metabolite in the extract of leaves, roots, stems and stem barks. The extract of Faloak stem barks showed the highest elastase inhibitory activity with IC 50 73.7 µg/mL. The total flavonoids and total polyphenol content of Faloak stem bark extract were respectively 28.75 mg/gram and 45.25 mg/gram extract. Conclusion: The 70% ethanol extract of leaves, roots, stems, and stem barks of Faloak showed elastase inhibitory activity, and stem barks extract showed the strongest activity. Faloak stem barks extract can be considered as potential to be developed as active compound in anti-aging product, both in cosmetic and pharmaceutical dosage forms.

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Elastase Activity Inhibition by the Most Active Fraction of Star Fruit (Averrhoa Carambola L.) Leaves From Three West Java Regions

Cited by 3 publications

References 17 publications

In vitro anti-aging activity of Muntingia calabura L. fruit extract and its fractions

In vitro anti-aging activity of Muntingia calabura L. fruit extract and its fractions

Unripe Fruit of Rubus Fraxinifolius as a Potential Source of Antioxidant and Antielastase Agent

Elastase Inhibitory Activity, Determination of Total Polyphenol and Determination of Total Flavonoids and Pharmacognosy Study of Faloak Plant (Sterculia quadrifida R.Br) from East Nusa Tenggara-Indonesia

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