Electrically promoted protein production by mammalian cells cultured on the electrode surface

Abstract

Protein production of mammalian cells has been promoted by applying a small constant potential to the surface of an electrode on which cells are cultured. Human carcinoma line of MKN45 cells were cultured on the surface of a platinum-coated plastic plate electrode. Low d.c. voltage of constant potential was applied to the electrode during 4-day culture to modulate the production of carcinoembryonic antigen (CEA). The amounts of both secreted and membrane-bound CEA were dependent on the applied potential during culture. Secreted CEA was more than twice in amount in the potential range from 0.2 V to 0.6 V vs. Ag/Agcl as compared with that of normal culture. In the potential range, CEA was also increased in membrane-bound form. The potential-controlled cell culture may have an enhanced effect on protein production.

The regulation of cell functions electrically using biodegradable polypyrrole–polylactide conductors

“…Electrical stimulation has shown its capacity to modulate cell membrane permeability [35] and cytoskeletal structure [36]. Furthermore, reduced cell membrane fluidity was reported during ES [36].…”

Electrical regulation of Schwann cells using conductive polypyrrole/chitosan polymers

“…The mechanism by which ES regulates Schwann cell functions is unclear. ES is capable of modulating cell membrane permeability,33 membrane fluidity, and cytoskeletal structure 34. All of these ES‐mediated effects are related to cell membrane structure.…”

Effect of electro-stimulation on activity of heterotrophic denitrifying bacteria and denitrification performance

“…Some studies pointed out that the electro-stimulation could affect microbial growth and activity, and proper electro-stimulation could promote microbial metabolism (Thrash and Coates, 2008). Kojima et al (1992) used 0.2-0.6 V potential to stimulate MKN45 cells (Carcinoma cells) in a culture dish with Pt as the anode and cathode, and showed that current stimulation could change the DNA, protein synthesis, membrane permeability and cell growth. Furthermore, heterotrophic bacterial viability was also observed to be not significantly affected when the applied electric current density was less than 6.2 A/m 2 , but was partly inactivated at current densities more than 12.3 A/m 2 in a membrane reactor (Wei et al, 2011).…”