Electrochemical detection of natural DNA damage induced by ferritin/ascobic acid/H2O2 system and amplification of DNA damage by endonuclease Fpg

Liu, Yi; Hu, Naifei

doi:10.1016/j.bios.2009.06.035

Cited by 29 publications

(5 citation statements)

References 44 publications

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“…In a Fenton reaction, Fe 2+ reacts with H 2 O 2 generating Fe 3+ and a very strong oxidizing agent—hydroxyl radical. The presence of Vit C allows the recycling of Fe 3+ back to Fe 2+ , which can subsequently catalyze the successive formation of hydroxyl radicals [ 1 , 150 ]. Moreover, it has also been shown that high concentrations of ascorbyl radical can reduce SOD activity.…”

Section: Role Of Vitamin C In Neurodegenerative Diseasesmentioning

confidence: 99%

Does Vitamin C Influence Neurodegenerative Diseases and Psychiatric Disorders?

et al. 2017

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Vitamin C (Vit C) is considered to be a vital antioxidant molecule in the brain. Intracellular Vit C helps maintain integrity and function of several processes in the central nervous system (CNS), including neuronal maturation and differentiation, myelin formation, synthesis of catecholamine, modulation of neurotransmission and antioxidant protection. The importance of Vit C for CNS function has been proven by the fact that targeted deletion of the sodium-vitamin C co-transporter in mice results in widespread cerebral hemorrhage and death on post-natal day one. Since neurological diseases are characterized by increased free radical generation and the highest concentrations of Vit C in the body are found in the brain and neuroendocrine tissues, it is suggested that Vit C may change the course of neurological diseases and display potential therapeutic roles. The aim of this review is to update the current state of knowledge of the role of vitamin C on neurodegenerative diseases including Alzheimer’s disease, Parkinson’s disease, Huntington’s disease, multiple sclerosis and amyotrophic sclerosis, as well as psychiatric disorders including depression, anxiety and schizophrenia. The particular attention is attributed to understanding of the mechanisms underlying possible therapeutic properties of ascorbic acid in the presented disorders.

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Section: Role Of Vitamin C In Neurodegenerative Diseasesmentioning

confidence: 99%

Does Vitamin C Influence Neurodegenerative Diseases and Psychiatric Disorders?

et al. 2017

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“…Until now, various H 2 O 2 physicochemical sensing strategies have been developed and extensively studied. − In comparison with other techniques, the electrochemical methods make great progress because of their intrinsic merits, such as the low detection limit, sound reliability, good selectivity, and simple apparatus. These characteristics provide the accessibility to perform the quick detection of different analytes, even directly within the biological specimens in real-time mode…”

mentioning

confidence: 99%

Detection of H₂O₂ at the Nanomolar Level by Electrode Modified with Ultrathin AuCu Nanowires

Wang

Han

et al. 2014

Anal. Chem.

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Bimetallic AuCu nanowires (AuCuNWs) are synthesized via a facile water solution method at room temperature. Enhanced electrocatalytic activity is observed toward the oxidation of H2O2, which makes the AuCu nanowire, along with its unique catalytic properties, intriguing bifunctional mechanism, and surface atomic construction, a promising platform for the amplification of interfacing signal. A highly sensitive H2O2 biosensor is thus developed on the base of the as-prepared AuCuNW catalyst. A very low real determination limit (2.0 nM) was reached, and a linear range as wide as 5 orders of magnitude was demonstrated. In addition, a trace amount of H2O2, which was released from Raw 264.7 cells, was selectively detected, hinting at the possible applications for real-time quantitative detection of H2O2 in a biological environment.

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“…Through the Fenton and Haber-Weiss reactions, H 2 O 2 generates OH - radicals [29,30] which inflict more than 100 different DNA modifications including 8,5'-cyclopurine-2'-deoxynucleosides (CyPudNs) [31], an oxidative lesion repaired by the NER but not the BER [18,19]. These findings suggest that unrepaired oxidative lesions may contribute to XP symptoms which cannot be accounted for by UV exposure alone such as internal cancers, developmental defects and neurodegeneration.…”

Section: Discussionmentioning

confidence: 99%

Hydrogen peroxide induced genomic instability in nucleotide excision repair-deficient lymphoblastoid cells

et al. 2010

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BackgroundThe Nucleotide Excision Repair (NER) pathway specialises in UV-induced DNA damage repair. Inherited defects in the NER can predispose individuals to Xeroderma Pigmentosum (XP). UV-induced DNA damage cannot account for the manifestation of XP in organ systems not directly exposed to sunlight. While the NER has recently been implicated in the repair of oxidative DNA lesions, it is not well characterised. Therefore we sought to investigate the role of NER factors Xeroderma Pigmentosum A (XPA), XPB and XPD in oxidative DNA damage-repair by subjecting lymphoblastoid cells from patients suffering from XP-A, XP-D and XP-B with Cockayne Syndrome to hydrogen peroxide (H2O2).ResultsLoss of functional XPB or XPD but not XPA led to enhanced sensitivity towards H2O2-induced cell death. XP-deficient lymphoblastoid cells exhibited increased susceptibility to H2O2-induced DNA damage with XPD showing the highest susceptibility and lowest repair capacity. Furthermore, XPB- and XPD-deficient lymphoblastoid cells displayed enhanced DNA damage at the telomeres. XPA- and XPB-deficient lymphoblastoid cells also showed differential regulation of XPD following H2O2 treatment.ConclusionsTaken together, our data implicate a role for the NER in H2O2-induced oxidative stress management and further corroborates that oxidative stress is a significant contributing factor in XP symptoms. Resistance of XPA-deficient lymphoblastoid cells to H2O2-induced cell death while harbouring DNA damage poses a potential cancer risk factor for XPA patients. Our data implicate XPB and XPD in the protection against oxidative stress-induced DNA damage and telomere shortening, and thus premature senescence.

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Electrochemical detection of natural DNA damage induced by ferritin/ascobic acid/H2O2 system and amplification of DNA damage by endonuclease Fpg

Cited by 29 publications

References 44 publications

Does Vitamin C Influence Neurodegenerative Diseases and Psychiatric Disorders?

Does Vitamin C Influence Neurodegenerative Diseases and Psychiatric Disorders?

Detection of H₂O₂ at the Nanomolar Level by Electrode Modified with Ultrathin AuCu Nanowires

Hydrogen peroxide induced genomic instability in nucleotide excision repair-deficient lymphoblastoid cells

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Electrochemical detection of natural DNA damage induced by ferritin/ascobic acid/H2O2 system and amplification of DNA damage by endonuclease Fpg

Cited by 29 publications

References 44 publications

Does Vitamin C Influence Neurodegenerative Diseases and Psychiatric Disorders?

Does Vitamin C Influence Neurodegenerative Diseases and Psychiatric Disorders?

Detection of H2O2 at the Nanomolar Level by Electrode Modified with Ultrathin AuCu Nanowires

Hydrogen peroxide induced genomic instability in nucleotide excision repair-deficient lymphoblastoid cells

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Detection of H₂O₂ at the Nanomolar Level by Electrode Modified with Ultrathin AuCu Nanowires