Electrochemical gradients are involved in regulating cytoskeletal patterns during epithelial morphogenesis in the Drosophila ovary

Weiß, Isabel; Bohrmann, Johannes

doi:10.1186/s12861-019-0203-y

Cited by 10 publications

(44 citation statements)

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“…4 and 5; for wt, cf. [8]). During S8, basal microfilaments (bMF) show the same parallel transversal alignment in wt and grk ( Fig.…”

Section: Cytoskeletal Differences Between Wt and Grkmentioning

confidence: 99%

“…Spatiotemporal electrochemical patterns affect cytoskeletal dynamics and play a role in defining spatial coordinates of tissues and organs in several species [1][2][3][4][5][6][7][8]. Therefore, it is tempting to investigate V mem -and pH igradients in relation to cytoskeletal patterns in a Drosophila mutant with disturbed axial polarity.…”

Section: Introductionmentioning

confidence: 99%

“…In particular, the cytoskeleton is an attractive candidate for bioelectrical signalling, since binding of actin-associated factors [27,28] as well as contractility of actomyosin complexes are controlled by pH i [29]. On the other hand, MT are known to amplify electrical signals [30,31], and modifications of the cytoskeletal organisation have been shown to be promoted by changes in V mem [8,32,33].…”

Section: Introductionmentioning

confidence: 99%

“…On the other hand, the organisation of microfilaments (MF) in the FE corresponds to FC differentiation and plays a decisive role in shaping the follicle along its longitudinal axis [38,39]. It has also been shown that pH i -and V mem -changes induced by several inhibitors of iontransport mechanisms located in the FE [7] simulate naturally occurring bioelectrical changes [4] and lead to alterations of MF-and MT-patterns as observed during FC differentiation [8]. Therefore, gradual modifications of electrochemical signals can serve as physiological means to regulate cell and tissue architecture by modifying cytoskeletal patterns [8].…”

Section: Introductionmentioning

confidence: 99%

“…In the present study, we compare wt and grk follicles with regard to their bioelectrical signals, using a fluorescent pH-indicator and a potentiometric dye [4,7]. In addition, we compare wt and grk follicles with regard to their cytoskeletal organisation, using fluorescent phalloidin and an antibody against acetylated α-tubulin [8].…”

Section: Introductionmentioning

confidence: 99%

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Bioelectrical and cytoskeletal patterns correlate with altered axial polarity in the follicular epithelium of the Drosophila mutant gurken

Schotthöfer

Bohrmann

2020

BMC Dev Biol

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Background: Bioelectrical signals are known to be involved in the generation of cell and tissue polarity as well as in cytoskeletal dynamics. The epithelium of Drosophila ovarian follicles is a suitable model system for studying connections between electrochemical gradients, patterns of cytoskeletal elements and axial polarity. By interactions between soma and germline cells, the transforming growth factor-α homolog Gurken (Grk) establishes both the anteroposterior and the dorsoventral axis during oogenesis. Results: In the follicular epithelium of the wild-type (wt) and the polarity mutant grk, we analysed stage-specific gradients of membrane potentials (V mem) and intracellular pH (pH i) using the potentiometric dye DiBAC 4 (3) and the fluorescent pH-indicator 5-CFDA,AM, respectively. In addition, we compared the cytoskeletal organisation in the follicular epithelium of wt and grk using fluorescent phalloidin and an antibody against acetylated α-tubulin. Corresponding to impaired polarity in grk, the slope of the anteroposterior V mem-gradient in stage S9 is significantly reduced compared to wt. Even more striking differences in V mem-and pH i-patterns become obvious during stage S10B, when the respective dorsoventral gradients are established in wt but not in grk. Concurrent with bioelectrical differences, wt and grk exhibit differences concerning cytoskeletal patterns in the follicular epithelium. During all vitellogenic stages, basal microfilaments in grk are characterised by transversal alignment, while wt-typical condensations in centripetal follicle cells (S9) and in dorsal centripetal follicle cells (S10B) are absent. Moreover, in grk, longitudinal alignment of microtubules occurs throughout vitellogenesis in all follicle cells, whereas in wt, microtubules in mainbody and posterior follicle cells exhibit a more cell-autonomous organisation. Therefore, in contrast to wt, the follicular epithelium in grk is characterised by missing or shallower electrochemical gradients and by more coordinated transcellular cytoskeletal patterns. Conclusions: Our results show that bioelectrical polarity and cytoskeletal polarity are closely linked to axial polarity in both wt and grk. When primary polarity signals are altered, both bioelectrical and cytoskeletal patterns in the follicular epithelium change. We propose that not only cell-specific levels of V mem and pH i , or the polarities of transcellular electrochemical gradients, but also the slopes of these gradients are crucial for cytoskeletal modifications and, thus, for proper development of epithelial polarity.

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“…4 and 5; for wt, cf. [8]). During S8, basal microfilaments (bMF) show the same parallel transversal alignment in wt and grk ( Fig.…”

Section: Cytoskeletal Differences Between Wt and Grkmentioning

confidence: 99%