Electrochemical studies of NADH oxidation on chemically reduced graphene oxide nanosheets modified glassy carbon electrode

Immanuel, Susan; Sivasubramanian, R.

doi:10.1016/j.matchemphys.2020.123015

Cited by 31 publications

(17 citation statements)

References 48 publications

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“…The limit of quantification (LOQ) was calculated to be 1.55 μM (S/N = 10). The method, limit of detection, and linear range of the modified electrode developed in this study were compared with those obtained with other NADH sensors in the literature; this information is presented in Table 1 [17,18,53,[55][56][57].…”

Section: Amperometric Determination Of Nadhmentioning

confidence: 99%

“…A lot of methods have been used for the accurate and reliable determination of NADH. For instance; fluorimetry [9], spectrophotometry [10], chromatography [11], chemiluminescence [12], electrochemiluminescence [13], and electroanalytical [14][15][16][17][18] techniques have been used for this purpose. Among these techniques, electroanalytical techniques have been remarkable and electrochemical determination of NADH using electrochemical methods has gained great importance.…”

Section: Introductionmentioning

confidence: 99%

“…To solve this problem, a lot of modified electrodes have been developed and researchers continue to develop new electrodes. To allow the electrochemical determination of NADH at low potential and minimize fouling on the electrode, effective electron transfer mediators and catalysts such as carbon nanotube, graphene, metal nanoparticles, polymer, and organic compounds have been used for electrode surface modification [17,[23][24][25][26]. However, these electrode materials have some disadvantages such as being expensive, long preparation processes, and requiring an expert in preparation.…”

Section: Introductionmentioning

confidence: 99%

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Sensitive Electrochemical Determination of NADH Using an Electrode Fabricated by Intercalation of Tetrabutylammonium Ions Into Graphite Electrode

Gördük

2021

Electroanalysis

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In this study, we present a strategy for the specific electrochemical detection of NADH in human blood serums using modified electrode fabricated by intercalation of tetrabutylammonium ions (TBA+) into pencil graphite electrode (TBA+/PGE). In order to implement this protocol, a modification process in the potential range (−0.2) to (−2.75) V was performed in DMSO containing 0.1 M TBAP by CV method. The characterization processes of the prepared sensor were carried out using CV, EIS, FT‐IR, XPS, and SEM methods. The electrode fabricated by intercalation of TBA+ was found to be able to analyze NADH at +0.32 V applied potential by the amperometric method. The LOD was detected as 0.46 μM. The analysis results of human blood serums which are taken from healthy individuals show that TBA+/PGE can be used for real samples. The results indicated that TBA+/PGE can be easily fabricated by one‐step and one‐pot electrochemical method and TBA+/PGE can be used as a potential sensor for the NADH determination.

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Section: Amperometric Determination Of Nadhmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Sensitive Electrochemical Determination of NADH Using an Electrode Fabricated by Intercalation of Tetrabutylammonium Ions Into Graphite Electrode

Gördük

2021

Electroanalysis

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“…Finally, unlike previous attempts that demonstrated photoelectrochemical regeneration of NADH 15 , we provide evidence here that illumination and electrochemistry need not be concurrent, implying that direct electrochemical regeneration can proceed without the undesirable production of the inactive dimer. Our facile, cheap, and direct electrochemical regeneration of NADPH, especially without any inactive dimer, is a useful advance in the regeneration of cofactors 18 , 19 .…”

Section: Introductionmentioning

confidence: 99%

Copper oxide-based cathode for direct NADPH regeneration

Kadowaki

Jones

Sengupta

et al. 2021

Sci Rep

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Nearly a fourth of all enzymatic activities is attributable to oxidoreductases, and the redox reactions supported by this vast catalytic repertoire sustain cellular metabolism. In many biological processes, reduction depends on hydride transfer from either reduced nicotinamide adenine dinucleotide (NADH) or its phosphorylated derivative (NADPH). Despite longstanding efforts to regenerate NADPH by various methods and harness it to support chemoenzymatic synthesis strategies, the lack of product purity has been a major deterrent. Here, we demonstrate that a nanostructured heterolayer Ni–Cu2O–Cu cathode formed by a photoelectrochemical process has unexpected efficiency in direct electrochemical regeneration of NADPH from NADP+. Remarkably, two-thirds of NADP+ was converted to NADPH with no measurable production of the inactive (NADP)2 dimer and at the lowest reported overpotential [− 0.75 V versus Ag/AgCl (3 M NaCl) reference]. Sputtering of nickel on the copper-oxide electrode nucleated an unexpected surface morphology that was critical for high product selectivity. Our results should motivate design of integrated electrolyzer platforms that deploy this heterogeneous catalyst for direct electrochemical regeneration of NADH/NADPH, which is central to design of next-generation biofuel fermentation strategies, biological solar converters, energy-storage devices, and artificial photosynthesis.

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“…The initial step results in the formation of NAD-radical through a one electron and a proton transfer process followed by successive second electron transfer to form NAD + . [24] In case of reduction, NAD + converts to NADH by a two-step process via the formation of NAD-radical as an intermediate. [25] During biocatalysis, NADH is supplied to the system for the synthesis of high value added compounds.…”

Section: Introductionmentioning

confidence: 99%

Recent Progress and Perspectives on Electrochemical Regeneration of Reduced Nicotinamide Adenine Dinucleotide (NADH)

Immanuel

Sivasubramanian

Gul

et al. 2020

Chemistry An Asian Journal

Self Cite

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NAD is a cofactor that maintains cellular redox homeostasis and has immense industrial and biological significance. It acts as an enzymatic mediator in several biocatalytic electrochemical reactions and undergoes oxidation/reduction to form NAD + or NADH, respectively. The NAD redox couple (NAD + /NADH) mostly exists in enzyme-assisted metabolic reactions as a coenzyme during which electrons and protons are transferred. NADH shuttles these charges between the enzyme and the substrate. In order to understand such complex metabolic reactions, it is vital to study the bio-electrochemistry of NADH. In addition, the regeneration of NADH in industries has attracted significant attention due to its vast usage and high cost. To make biocatalysis economically viable, primary methods of NADH regeneration including enzymatic, chemical, photochemical and electrochemical methods are widely used. This review is mainly focused on the electrochemical reduction of NAD + to NADH with specific details on the mechanism and kinetics of the reaction. It provides emphasis on the different routes (direct and mediated) to electrochemically regenerate NADH from NAD + highlighting the NAD dimer formation. Also, it describes the electrocatalysts developed until now and the scope for development in this area of research.

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Electrochemical studies of NADH oxidation on chemically reduced graphene oxide nanosheets modified glassy carbon electrode

Cited by 31 publications

References 48 publications

Sensitive Electrochemical Determination of NADH Using an Electrode Fabricated by Intercalation of Tetrabutylammonium Ions Into Graphite Electrode

Sensitive Electrochemical Determination of NADH Using an Electrode Fabricated by Intercalation of Tetrabutylammonium Ions Into Graphite Electrode

Copper oxide-based cathode for direct NADPH regeneration

Recent Progress and Perspectives on Electrochemical Regeneration of Reduced Nicotinamide Adenine Dinucleotide (NADH)

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