Electrochemiluminescence Based Enzymatic Urea Sensor Using Nanohybrid of Isoluminol‐gold Nanoparticle‐graphene Oxide Nanoribbons

Ismail, Nur; Hoa, Le Quynh; Huong, Vu Thi; Inoue, Yuki; Yoshikawa, Hiroyuki; Saito, Masato; Tamiya, Eiichi

doi:10.1002/elan.201600477

Cited by 23 publications

(15 citation statements)

References 26 publications

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“…We have paid attentions on ECL [15, 16] and developed various biosensors based on the ability of luminol electrochemiluminescence to measure reactive oxygen species [17]. These techniques were applied to the measurement of antioxidants [18], glycated albumin [19], catalase activity [20], urea [21] and neutrophil reactive oxygen species [22]. Regarding catalytic effects of gold nanoparticles were able to be detected by the ECL sensing with luminol.…”

Section: Figurementioning

confidence: 99%

Gold Nanocatalysts Towards Digital Sensing Probes with Electrochemiluminescence Based Micro Electrodes Array

et al. 2021

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The authors have found that gold nanoparticles (AuNP) function as nanocatalysts for the generation of reactive oxygen species such as hydrogen peroxide from dissolved oxygen in coexistence with tris(hydroxymethyl)aminomethane (Tris) or N‐[tris(hydroxymethyl) methyl]glycine (Tricine) chemicals, and that these can be evaluated by luminol electrochemi‐luminescence (ECL). In the present study, we investigated the effects of reaction time, particle concentration, and temperature on the catalytic properties of gold nanoparticles. Unlike biocatalysts such as enzymes, the gold nanoparticles showed an Arrhenius plot‐like increase in activity with temperature without inactivation. In addition, the catalytic activity of the gold nanoparticles varied with the size and shape of the nanoparticles for spherical gold nanoparticles of different sizes (5, 15, 30, 50 and 400 nm) and the activity per nanoparticle increased with the size and surface area. When compared activity per surface area, 15 nm nanoparticles showed higher activity. We prepared gold nanoparticles with nanoflower (AuNF) structure of approx.200 nm in size which showed higher activity per particle than 400 nm spherical particles. The increase in surface area and the difference in surface condition may have influenced these results. We also prepared microelectrode arrays capable of holding small amounts of nl‐pL test reagents. They showed the possibility of imaging ECL of the activity of gold nanoparticles at the level of tens of nanoparticles which could lead to nanocatalysts as probes for digital analysis.

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Section: Figurementioning

confidence: 99%

Gold Nanocatalysts Towards Digital Sensing Probes with Electrochemiluminescence Based Micro Electrodes Array

et al. 2021

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“…Similar to the role of hydrogen peroxide as an intermediate product of many enzymatic reactions, variable pH values can indicate some enzyme-catalyzed reactions of urease, 15 acetyl cholinesterase 16 and glucose oxidase. 17 For example, urease catalyze and decompose urea into products of NH 4 + , HCO 3 − , and OH − , 18 which causes the increase of pH values.…”

Section: Introductionmentioning

confidence: 99%

A fluorescent sensor constructed from nitrogen-doped carbon nanodots (N-CDs) for pH detection in synovial fluid and urea determination

et al. 2018

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Blue luminescent nitrogen-doped carbon nanodots (N-CDs) with pH-dependent properties were prepared from citric acid (CA), glutathione (GSH), and polyethylene polyamine (PEPA) using a two-step pyrolytic route. The N-CDs showed stable and strong emission bands at approximately 455 nm under 350 nm excitation. Moreover, the fluorescence of N-CDs can be gradually decreased by gradually increasing the pH value. A good linear relationship between the fluorescence intensity of N-CDs and the pH range of 3.0-9.0 was obtained. Thus, the response mechanism of N-CDs to pH was systematically investigated.N-CDs possessed -NH 2 , -COOH, and -CONHas active functional groups, which allowed the variable protonation/deprotonation of N-CDs to regulate the fluorescence emission intensities under changed pH values. Furthermore, upon combining urease-catalyzed hydrolysis of urea with increased pH values, a simple but effective fluorescence assay for urea was developed. The analytical performance for urea detection was the linear range of 0 to 10 mM with a detection limit of 0.072 mM. Additionally, the fluorescent sensor based on N-CDs was successfully applied for pH detection in synovial fluid and urea determination in serum. † Electronic supplementary information (ESI) available: Stability of N-CDs in salt solution, high-resolution XPS spectra of C1s and O1s, inuence of pH to pure CDs, cycle times of the reversible pH-response of N-CDs, tables of the comparison in the analytical performance of pH detection and urea determination. See

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“…These characteristics make ECL a promising tool for various biosensing applications [1][2][3][4][5]. In this work, to achieve highly sensitive biosensing based on ECL (Figures 1 and 2), we focused on gold nanoparticle (AuNP) catalysis.…”

Section: Introductionmentioning

confidence: 99%

Electrochemiluminscence Based Biosensors with AuNP Showing Catalytic ROS Generation

Higashi

Mazumder

Yoshikawa

et al. 2017

Proceedings of Eurosensors 2017, Paris, France, 3–6 September 2017

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Electrochemiluminescence (ECL) has been a useful analytical tool for fields such as molecular biology, food analysis, and analytical chemistry. In situ generation of reactive oxygen species (ROS) by surface mediated catalysis works collaboratively with the luminol based ECL system. This work describes an ECL strategy involving in situ ROS generation from the surface of gold nanoparticles (AuNP). We reported on a novel condition to induce AuNP catalysis, and examined the application of this phenomenon to an immunosensing platform based on AuNP catalysis and ECL.

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Electrochemiluminescence Based Enzymatic Urea Sensor Using Nanohybrid of Isoluminol‐gold Nanoparticle‐graphene Oxide Nanoribbons

Cited by 23 publications

References 26 publications

Gold Nanocatalysts Towards Digital Sensing Probes with Electrochemiluminescence Based Micro Electrodes Array

Gold Nanocatalysts Towards Digital Sensing Probes with Electrochemiluminescence Based Micro Electrodes Array

A fluorescent sensor constructed from nitrogen-doped carbon nanodots (N-CDs) for pH detection in synovial fluid and urea determination

Electrochemiluminscence Based Biosensors with AuNP Showing Catalytic ROS Generation

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