Electrospun biodegradable polyorganophosphazene fibrous matrix with poly(dopamine) coating for bone regeneration

Abstract

Biodegradable polyphosphazenes were categorized as osteoinductive materials because of their phosphorus-containing feature; however, they were less supportive in cell attachment and proliferation at earlier points in comparison with biodegradable aliphatic polyesters. Therefore, mussel-inspired surface modification of poly(alanine ethyl ester-co-glycine ethyl ester)phosphazene (PAGP) was studied, intending to circumvent the above-mentioned disadvantage of polyphosphazene. To this end, PAGP and poly(L-lactide) (PLLA) were electrospun into nanofibrous substrates and surface treated with dopamine aqueous solution. With the analysis of scanning electron microscope, transmission electron microscope, X-ray photoelectron spectroscope, and Fourier transform infrared spectroscope, the successful poly(dopamine) coating was identified on both PAGP and PLLA nanofibers. MC3T3-E1 osteoblasts were found attaching and proliferating much well on poly(dopamine)-modified nanofibrous substrates in comparison with the pristine ones. In addition, the poly(dopamine) coating demonstrated high activity in promoting osteogenous differentiation. Because the phosphorus content on nanofiber surface was decreased with the poly(dopamine) coating, the poly(dopamine)-coated PAGP nanofibrous substrate was slightly inferior to pure PAGP nanofibrous substrate in osteogenous differentiation. In a summary, the results confirmed that poly(dopamine)-modified polyphosphazenes were promising scaffold materials with both high cell affinity and high osteocompatibility for bone regeneration.

Chitosan and hydroxyapatite composite cross‐linked by dopamine has improved anisotropic hydroxyapatite growth and wet mechanical properties

“…The other possible reason for the reduced cell proliferation is relatively high concentration of dopamine (∼0.8 M). Generally, the addition of dopamine increased the cell proliferation when the dopamine used to generate poly(dopamine) coating with low dopamine concentration (<0.02 M) . In contrast, the cell proliferation was slightly reduced when the dopamine used as a cross‐linker with high concentration as in this study (>0.5 M) .…”

Enhance the Bioactivity and Osseointegration of the Polyethylene‐Terephthalate‐Based Artificial Ligament via Poly(Dopamine) Coating with Mesoporous Bioactive Glass

“…Secondly, the MBGs are hydrophilicity and have large specific area and pore structure, which may enhance the hydrophilicity of the coating materials. In the study of Li Yan, after the dopamine solution treatment, the water contact angles of as‐spun PAGP and PLLA decreased . Here, we put an example of increased hydrophobicity via dopamine coating.…”

Flexible fiber-reinforced composites with improved interfacial adhesion by mussel-inspired polydopamine and poly(methyl methacrylate) coating

“…Blank domains with no coating material could be found when DA solution of low concentration was used, whereas excess patches could be found if the DA solution had a relatively high concentration. In previous studies, it was found that a DA solution of 2 mg/ml was quite efficient in obtaining a homogenous PDA surface layer by coating PDA on fiber post [33] or polymeric nanofibers [34]. Therefore, with all the morphological comparisons on PDA-modified glass fibers in Fig.…”