Embryo maturity plays an important role for the successful cryopreservation of coconut (Cocos nucifera)

Sisunandar, Sisunandar; Novarianto, Hengky; Mashud, Nurhaini; Samosir, Y.; Adkins, Steve W.

doi:10.1007/s11627-014-9633-1

Cited by 12 publications

(5 citation statements)

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“…In the 1980s, the first attempt to cryopreserve coconut tissues was undertaken with immature zygotic embryos using a chemical dehydration and slow freezing technique (Bajaj 1984). However, more recently attention has shifted towards using mature (11 months post-pollination) zygotic embryos (Sisunandar et al 2014) and using a physical dehydration method; or using plumule tissues excised from mature zygotic embryos and using a chemical dehydration method (Supplement 1). As with most species the cryopreservation protocol for coconut consists of four steps: firstly the pre-culture of the explanted tissues in preparation for drying; secondly tissue dehydration; thirdly tissue freezing; and finally tissue recovery involving thawing and plantlet production.…”

Section: Germplasm Conservation Via Cryopreservationmentioning

confidence: 99%

Tissue culture and associated biotechnological interventions for the improvement of coconut (Cocos nucifera L.): a review

Nguyen

Bandupriya

López‐Villalobos

et al. 2015

Planta

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The present review discusses not only advances in coconut tissue culture and associated biotechnological interventions but also future research directions toward the resilience of this important palm crop. Coconut (Cocos nucifera L.) is commonly known as the 'tree of life'. Every component of the palm can be used to produce items of value and many can be converted into industrial products. Coconut cultivation faces a number of acute problems that reduce its productivity and competitiveness. These problems include various biotic and abiotic challenges as well as an unstable market for its traditional oil-based products. Around 10 million small-holder farmers cultivate coconut palms worldwide on c. 12 million hectares of land, and many more people own a few coconut palms that contribute to their livelihoods. Inefficiency in the production of seedlings for replanting remains an issue; however, tissue culture and other biotechnological interventions are expected to provide pragmatic solutions. Over the past 60 years, much research has been directed towards developing and improving protocols for (i) embryo culture; (ii) clonal propagation via somatic embryogenesis; (iii) homozygote production via anther culture; (iv) germplasm conservation via cryopreservation; and (v) genetic transformation. Recently other advances have revealed possible new ways to improve these protocols. Although effective embryo culture and cryopreservation are now possible, the limited frequency of conversion of somatic embryos to ex vitro seedlings still prevents the large-scale clonal propagation of coconut. This review illustrates how our knowledge of tissue culture and associated biotechnological interventions in coconut has so far developed. Further improvement of protocols and their application to a wider range of germplasm will continue to open up new horizons for the collection, conservation, breeding and productivity of coconut.

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Section: Germplasm Conservation Via Cryopreservationmentioning

confidence: 99%

Tissue culture and associated biotechnological interventions for the improvement of coconut (Cocos nucifera L.): a review

Nguyen

Bandupriya

López‐Villalobos

et al. 2015

Planta

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“…It is reported that embryos isolated from an 11 months old fruit gave the highest number of normal seedlings (~28%) when compared to counterparts excised from younger fruits (Sisunandar et al 2014). In addition, Sisunandar et al (2014) also reported that nuts could be stored up to three weeks prior to embryo isolation. The optimum post-harvest storage duration of nuts before using zygotic embryos in cryopreservation has been highlighted in this study.…”

Section: Effect Of Embryo Maturity and Duration Of Storage For Cryopreservationmentioning

confidence: 99%

“…For example, pre-growth treatments on media with high sucrose concentration may increase the tolerance to desiccation, and thus reduce the extent of damage when dehydrating and freezing (Engelmann et al, 1995). Sisunandar et al (2014) also had employed dehydration methods to investigate the effect of embryo maturity on cryopreservation success in 'Nias Yellow Dwarf', 'Tebing Tinggi Dwarf', 'Takome Tall', and 'Bali Tall' cultivars. It is reported that embryos isolated from an 11 months old fruit gave the highest number of normal seedlings (~28%) when compared to counterparts excised from younger fruits (Sisunandar et al 2014).…”

Section: Effect Of Embryo Maturity and Duration Of Storage For Cryopreservationmentioning

confidence: 99%

“…Sisunandar et al (2014) also had employed dehydration methods to investigate the effect of embryo maturity on cryopreservation success in 'Nias Yellow Dwarf', 'Tebing Tinggi Dwarf', 'Takome Tall', and 'Bali Tall' cultivars. It is reported that embryos isolated from an 11 months old fruit gave the highest number of normal seedlings (~28%) when compared to counterparts excised from younger fruits (Sisunandar et al 2014). In addition, Sisunandar et al (2014) also reported that nuts could be stored up to three weeks prior to embryo isolation.…”

Section: Effect Of Embryo Maturity and Duration Of Storage For Cryopreservationmentioning

confidence: 99%

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Coconut Cryopreservation: Present Status and Future Prospects

Welewanni¹,

Bandupriya²

2017

CORD

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Coconut is one of the most important small holder crops worldwide. Conservation of coconut as seeds or field gene banks is not effective due to a range of limitations. Cryopreservation, which is the conservation of living propagules at very low temperature (-196ºC), is the only method available currently for the long-term conservation of germplasm for problem plant species such as recalcitrant and vegetatively propagated plant species. This review summarizes different cryopreservation techniques that have been published from 1984 until the present in relation to different coconut material; it includes a brief discussion about short and medium-term cryopreservation before describing long-term preservation. It discusses factors affecting the process and success of cryopreservation, such as selection of plant material, pre-culture of tissues, osmoprotection, dehydration, cryo-storage, thawing and post-culturing of tissues, and finally to plants. The review also describes histological and ultra-structural studies on and the use of molecular markers to assess genetic stability after cryopreservation. Limitations and future directions related to coconut cryopreservation are discussed. Additional experiments are identified that will need to be undertaken to improve our understanding of the different cryopreservation methods.

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“…These dwarf coconut variants have become popular due to a scarcity of coconut climbers. Tebing Tinggi Dwarf (TTD), Bali Yellow Dwarf (BYD), Nias Yellow Dwarf (NYD), Jombang Green Dwarf (JGD), Nias Green Dwarf (NGD), Waingapu Red Dwarf (WRD), Raja Brown Dwarf (RBD), Sagerat Orange Dwarf (SOD), Salak Green Dwarf (SGD), and Hybrid Coconut (KHINA 1) are the noteworthy dwarf coconut kinds (Sopade, Samosir, Rival, & Adkins, 2010;Novarianto, Mashud, Samosir, & Adkins, 2014;Welewanni & Bandupriya, 2017;. In Brazil, dwarf variants are commonly employed for economic purposes.…”

Section: Dwarf Coconuts Plantationsmentioning

confidence: 99%

Coconut Tree (Cocos nucifera) Products: A Review of Global Cultivation and its Benefits

Henrietta

Kalaiyarasi

Raj

2022

J. Stabil. Environ. Mgt

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The coconut tree (Cocos nucifera) is one of the most extensively and widely used palm trees. The coconut palm is regarded as the "tree of life," or "Kalpavriksha." Humans have been cultivating a wide range of coconut trees around the world. This study is based on analysis of secondary data on cultivation of coconut trees and its benefits. Due to increased insect infestations and a changing habitat, the lifespan of coconut trees have been affected. Coconut product market has become the fastest expanding business due to the anti-viral compounds found in it. The coconut is used to produce oils, even the shells used for craft materials. People have been using coconut trees to make brooms and woods are being used to make furniture, construction materials for dwellings, and hardwood floors. It shows the livelihood of people around the world have been changed due to the cultivation of coconut trees. Indonesia, India and Philippines are top three countries to be economically benefitted from the plantation of coconut trees. As there is high demand of coconut based products worldwide, farmers need to be made aware about this economic value for increment in production.

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Embryo maturity plays an important role for the successful cryopreservation of coconut (Cocos nucifera)

Cited by 12 publications

References 14 publications

Tissue culture and associated biotechnological interventions for the improvement of coconut (Cocos nucifera L.): a review

Tissue culture and associated biotechnological interventions for the improvement of coconut (Cocos nucifera L.): a review

Coconut Cryopreservation: Present Status and Future Prospects

Coconut Tree (Cocos nucifera) Products: A Review of Global Cultivation and its Benefits

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