Emergence of a highly pathogenic porcine reproductive and respiratory syndrome virus in the Mid-Eastern region of China

Li, Yufeng; Wang, Xinglong; Bo, Kuntao; Wang, Xianwei; Tang, Bo; Yang, Baoshou; Jiang, Wenming; Jiang, Ping

doi:10.1016/j.tvjl.2007.07.032

Cited by 285 publications

(197 citation statements)

References 29 publications

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“…4a, b). A unique 30 amino acid discontinuous deletion at position 481 and 533 to 561 in Nsp2 protein has been considered as the genetic marker for highly pathogenic variant of PRRSV [18,29,30]. Therefore, our results confirm the Indian PRRSV as HP-PRRSV of Chinese origin.…”

Section: Discussionsupporting

confidence: 75%

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Indian porcine reproductive and respiratory syndrome virus bears discontinuous deletion of 30 amino acids in nonstructural protein 2

et al. 2016

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Since its first outbreak in 2013, porcine reproductive and respiratory syndrome (PRRS) has established as an enzootic disease in pig population of Mizoram state, India. Our previous studies based on phylogenetic analysis of ORF5 and ORF7 gene sequences revealed close relationship of Indian PRRSV with the highly pathogenic variant of PRRSV (HP-PRRSV) of Chinese origin. Despite the control measures, second major outbreak of the disease was recorded in Aizawl district of Mizoram in 2015. The objective of the present study was to examine the origin of PRRSV isolates of 2015 outbreak, identification of deleted region in Nsp2 gene and determination of any genetic variation between 2013 and 2015 isolates of PRRSV. The outbreak was confirmed by the detection of PRRSVspecific antibodies in 57 out of 92 serum samples (61.96 %) and also by RT-PCR in 42 out of 42 necropsy samples (100 %). Nucleotide sequence analysis of Nsp2 coding region of Indian isolates and comparison with reference sequences revealed 90 nucleotides discontinuous deletion further establishes the closeness of Indian PRRSV to Chinese HP-PRRSV. Further, sequence and phylogenetic analysis of ORF5, ORF7 and Nsp2 genes of Indian PRRSV from both 2013 and 2015 revealed that the outbreaks were caused by two different strains of HP-PRRSV closely associated with the Chinese 10 HEB-3 isolate and 07QN isolates of Vietnam origin respectively. The present study confirms that the Indian PRRSV is a highly pathogenic variant of PRRSV and this study serves as the basis for developing practical and effective control measures against this disease.

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Section: Discussionsupporting

confidence: 75%

“…The causative agent, HP-PRRS virus (HP-PRRSV) is a novel variant of PRRSV containing a unique discontinuous deletion of 30 amino acids (aa) in nonstructural protein 2 (Nsp2) [18,29,30]. Recent studies have suggested that HP-PRRSV has evolved from Chinese classical type 2 PRRSV like CH1a isolate [2].…”

Section: Introductionmentioning

confidence: 99%

Indian porcine reproductive and respiratory syndrome virus bears discontinuous deletion of 30 amino acids in nonstructural protein 2

et al. 2016

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“…The temperature peak and infection kinetics of the recombinant virus-inoculated groups were similar to those of the parental virus-infected group (P,0.05). All virusinoculated pigs developed PHFS clinical symptoms beginning at 5 days p.i., displaying lethargy, lack of appetite, coughing and often paralysis, as reported by others Li et al, 2007;Tian et al, 2007). Remarkably, all virus-inoculated pigs had died by 13-16 days p.i.…”

supporting

confidence: 66%

An infectious cDNA clone of a highly pathogenic porcine reproductive and respiratory syndrome virus variant associated with porcine high fever syndrome

Zhang

Sun

et al. 2008

Journal of General Virology

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Since May 2006, a so-called ‘porcine high fever syndrome’ (PHFS) has spread all over China. The arterivirus porcine reproductive and respiratory syndrome virus (PRRSV) was believed to be the main causative agent, although the involvement of other pathogens was not formally excluded. The genome of a representative Chinese PRRSV strain, named JX143, was sequenced and used to develop infectious cDNA clones, pJX143 and pJX143M, with the latter containing an engineered MluI site that served as a genetic marker. In various virological assays, the rescued viruses, vJX143 and vJX143M, were indistinguishable from their parental virus. Animal experiments showed that these recombinant viruses retained the high pathogenicity and induced the typical clinical symptoms observed during PHFS outbreaks. This is the first report describing infectious cDNA clones of this highly pathogenic PRRSV. Our results unambiguously fulfil Koch's postulates and define highly pathogenic PRRSV as the aetiological agent of PHFS in China.

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“…The recent emergence of highly pathogenic strains of PRRS virus in North America (Han et al, 2006) and especially in Asia (Li et al, 2007;Normile, 2007;Tian et al, 2007;Tong et al, 2007), as well as reports of greater pathogenicity associated with at least one eastern European virus (Karniychuk et al, 2010;StockhofeZurwieden et al, 2011), has highlighted the potential for ongoing changes in the virus to result in highly pathogenic mutants. This demonstrates a need to monitor the evolution of existing virus strains in order to detect the emergence of any with such an increased virulence.…”

Section: Discussionmentioning

confidence: 99%

Porcine reproductive and respiratory syndrome virus: Genetic diversity of recent British isolates

Frossard

Hughes

Westcott

et al. 2013

Veterinary Microbiology

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Emergence of a highly pathogenic porcine reproductive and respiratory syndrome virus in the Mid-Eastern region of China

Cited by 285 publications

References 29 publications

Indian porcine reproductive and respiratory syndrome virus bears discontinuous deletion of 30 amino acids in nonstructural protein 2

Indian porcine reproductive and respiratory syndrome virus bears discontinuous deletion of 30 amino acids in nonstructural protein 2

An infectious cDNA clone of a highly pathogenic porcine reproductive and respiratory syndrome virus variant associated with porcine high fever syndrome

Porcine reproductive and respiratory syndrome virus: Genetic diversity of recent British isolates

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