2020
DOI: 10.3390/v12020149
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Emerging PCR-Based Techniques to Study HIV-1 Reservoir Persistence

Abstract: While current antiretroviral therapies are able to halt HIV-1 progression, they are not curative, as an interruption of treatment usually leads to viral rebound. The persistence of this stable HIV-1 latent reservoir forms the major barrier in HIV-1 cure research. The need for a better understanding of the mechanisms behind reservoir persistence resulted in the development of several novel assays allowing to perform an extensive in-depth characterization. The objective of this review is to present an overview o… Show more

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Cited by 21 publications
(21 citation statements)
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“…Several sequencing-based assays have been developed to study the HIV reservoir, each focusing on different aspects of the infected cells and the proviruses within 27 . Near full-length (NFL) provirus sequencing enables the identification of genomeintact and potentially replication-competent proviruses [28][29][30][31] .…”
Section: Introductionmentioning
confidence: 99%
“…Several sequencing-based assays have been developed to study the HIV reservoir, each focusing on different aspects of the infected cells and the proviruses within 27 . Near full-length (NFL) provirus sequencing enables the identification of genomeintact and potentially replication-competent proviruses [28][29][30][31] .…”
Section: Introductionmentioning
confidence: 99%
“…Previously, full-length sequencing of proviral DNA has provided invaluable insight into the composition of the LR (Ho et al, 2013) but the methods used are time consuming and technically challenging. The advent of various NGS technologies, however, has also paved the way for novel methods to measure the HIV-1 LR with relative ease and high throughput (Lambrechts et al, 2020). The use of Illumina based sequencing techniques has so far been used in LR studies to measure fulllength, individual proviral sequences, helping to elucidate the driving force of LR persistence and latency maintenance (Hiener et al, 2017;Lee et al, 2017;Einkauf et al, 2019).…”
Section: Bridging the Gap Between Culture And Pcr Based Assaysmentioning
confidence: 99%
“…The functionality of latent reservoirs is in large part determined by the intactness of the proviral sequences. Therefore, efforts were made to develop more sensitive and accurate assays to obtain full sequencing of the HIV-1 genome in patient samples ( Lambrechts et al, 2020 ). Remarkably, only 10% of the proviral sequences exhibit an intact genome and are thus theoretically able to produce replication-competent virus.…”
Section: Introductionmentioning
confidence: 99%