Tear samples of 20 patients who were determined to have positive hepatitis B surface antigen (HBsAg) after an acute hepatitis B infection and aqueous humor samples of 10 patients who were HBsAg carriers were tested for HBsAg, and the results obtained for serum and these fluids were compared. In 85% of the 20 patients who were HBsAg positive, HBsAg was found to be positive in tear samples. In 50% of the other 10 patients in aqueous humor was also found to be HBsAg positive. Our findings show that tears and aqueous humor are important in the spreading of hepatitis B infections.