Endothelial glycoconjugates: a comparative lectin study of the brain, retina and myocardium

Lawrenson, John G; Cassella, John; Hayes, Alan; Firth, J. A.; Allt, G.

doi:10.1046/j.1469-7580.2000.19610055.x

Cited by 20 publications

(8 citation statements)

References 25 publications

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“…Wheat germ agglutinin (WGA) also labeled numerous N-acetyl-glucosamines and Nacetyl-neuraminic acids present on the endothelium, but α-fucose was not detected in the brain. These findings were also confirmed by another study [154]. Not only healthy cells, but also malignant cells exhibit a special pattern of sugar moieties on their surface, making lectins good candidates for the targeting of drugs.…”

Section: Coating With Lectinssupporting

confidence: 77%

Exploiting the Properties of Biomolecules for Brain Targeting of Nanoparticulate Systems

Cramer

Rempe²,

Galla³

2012

CMC

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The main obstacle in the treatment of central nervous system diseases is represented by a limited passage of diagnostic and therapeutic agents across the blood-brain barrier, which separates the blood stream from the cerebral parenchyma and maintains the homeostasis of the brain. The growing knowledge about the brain capillary endothelium and the discovery of specific mechanisms for the uptake of substances enables the development of various strategies to enhance the drug delivery rate into the brain. Among the different strategies, nanoparticles are promising candidates for drug delivery to the brain due to their potential in encapsulating drugs and thereby disguising their permeation limiting characteristics. Furthermore a surface functionalization of many nanoparticles can easily be achieved allowing the active targeting of nanoparticles to the brain. For this non-invasive approach, the surface functionalization of nanoparticles with biomolecules has shown promising potential for effective drug delivery to the brain. This review indexes the main classes of biomolecules used for the surface functionalization of nanoparticles and discusses their potential as drug delivery systems for an enhanced passage of diagnostic and therapeutic agents into the brain parenchyma.

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Section: Coating With Lectinssupporting

confidence: 77%

Exploiting the Properties of Biomolecules for Brain Targeting of Nanoparticulate Systems

Cramer

Rempe²,

Galla³

2012

CMC

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“…The connective tissue was least labelled by SNA-I recognizing sialic acid in the α 2-6 glycosidic linkage (Lawrenson et al 2000). However, MAA that labels sialic acid in α 2-3 linkage to β-galactose stained connective tissue and basement membranes of muscle fibres intensely.…”

Section: Discussionmentioning

confidence: 99%

Lectin histochemistry of microvascular endothelium in chick and quail musculature

Naňka

Peumans

Damme

et al. 2001

Anatomy and Embryology

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The lectin binding pattern of muscular microvessels in chick, quail and chick/quail chimeras was analysed. Paraffin wax sections of muscles from embryonic and adult animals were used. The biotin-labelled lectins were detected by avidin-alkaline phosphatase complex. The following lectins bound to muscular microvessels including arterioles, capillaries and venules of both species: SNA-I (Sambucus nigra agglutinin), MAA (Maackia amurensis agglutinin), AIA (Artocarpus integrifolia agglutinin), VAA-I, VAA-II and VAA-III (Viscum album agglutinin I-III), WGA (wheat germ agglutinin), LEA (Lycopersicon esculentum agglutinin). Endomysium and basement membranes of muscle fibres were also stained to a variable extent and intensity. Only SNA-I stained almost exclusively the endothelium of blood vessels. WFA (Wisteria floribunda agglutinin) bound to the quail endothelium only. MPA (Maclura pomifera agglutinin) marked vessels in adult muscles of chick and quail, but embryonic vessels were stained in quail only. Our results show that lectin histochemistry is a useful tool for visualisation of microvasculature in avian species. In particular, WFA and MPA can be used to determine the origin of endothelia in chick/quail chimeras.

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“…Although not specifically studied, retinal endothelial cells are likely to assist mural myocytes and pericytes in sensing and transducing mechanical forces. The retinal endothelium is coated with a glycocalyx (Lawrenson et al, 2000), which has been shown to function as a mechanical sensor and transducer in extra-ocular endothelia (Tarbell and Ebong, 2008). Retinal vascular endothelium also has the ability to detect chemical perturbations, including hypoxia and hypercapnia, and the metabolite, lactose (Pournaras et al, 2008).…”

Section: Anatomical and Physiological Considerationsmentioning

confidence: 99%

Role of the retinal vascular endothelial cell in ocular disease

Bharadwaj

Appukuttan

Wilmarth

et al. 2013

Progress in Retinal and Eye Research

142

114

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Retinal endothelial cells line the arborizing microvasculature that supplies and drains the neural retina. The anatomical and physiological characteristics of these endothelial cells are consistent with nutritional requirements and protection of a tissue critical to vision. On the one hand, the endothelium must ensure the supply of oxygen and other nutrients to the metabolically active retina, and allow access to circulating cells that maintain the vasculature or survey the retina for the presence of potential pathogens. On the other hand, the endothelium contributes to the blood-retinal barrier that protects the retina by excluding circulating molecular toxins, microorganisms, and pro-inflammatory leukocytes. Features required to fulfill these functions may also predispose to disease processes, such as retinal vascular leakage and neovascularization, and trafficking of microbes and inflammatory cells. Thus, the retinal endothelial cell is a key participant in retinal ischemic vasculopathies that include diabetic retinopathy and retinopathy of prematurity, and retinal inflammation or infection, as occurs in posterior uveitis. Using gene expression and proteomic profiling, it has been possible to explore the molecular phenotype of the human retinal endothelial cell and contribute to understanding of the pathogenesis of these diseases. In addition to providing support for the involvement of well-characterized endothelial molecules, profiling has the power to identify new players in retinal pathologies. Findings may have implications for the design of new biological therapies. Additional progress in this field is anticipated as other technologies, including epigenetic profiling methods, whole transcriptome shotgun sequencing, and metabolomics, are used to study the human retinal endothelial cell.

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Endothelial glycoconjugates: a comparative lectin study of the brain, retina and myocardium

Cited by 20 publications

References 25 publications

Exploiting the Properties of Biomolecules for Brain Targeting of Nanoparticulate Systems

Exploiting the Properties of Biomolecules for Brain Targeting of Nanoparticulate Systems

Lectin histochemistry of microvascular endothelium in chick and quail musculature

Role of the retinal vascular endothelial cell in ocular disease

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