Engineering Escherichia coli membrane phospholipid head distribution improves tolerance and production of biorenewables

Tan, Zhongyang; Khakbaz, Pouyan; Chen, Yingxi; Lombardo, Jeremy; Yoon, Jong Moon; Shanks, Jacqueline V.; Klauda, Jeffery B.; Jarboe, Laura R.

doi:10.1016/j.ymben.2017.08.006

Cited by 93 publications

(62 citation statements)

References 70 publications

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“…Different from traditional metabolic engineering, reverse engineering can quickly locate the key target via metabolic model prediction (Lu et al, ) and multiple omics approaches (Walker et al, ), rather than extensive screening of metabolic pathway genes. Furthermore, membrane‐lipid‐associated genes reported to influence stress tolerance include cis‐trans isomerase Cti (Tan et al, ), PS synthase PssA (Tan et al, ), and desaturase Ole1 (Degreif et al, ). In this study, membrane phospholipid genes cds1 and cho1 were overexpressed, which effectively enhances tolerance.…”

Section: Discussionmentioning

confidence: 99%

“…Similar to cho1, PssA is a gene in Escherichia coli that encodes a PS synthase. PssA overexpression increases PE zwitterionic phospholipid content (Tan et al, ). Other methods of regulating membrane lipids include transcription factors such as Rds2 and Crz1 (Wu et al, ; Yan et al, ) and mediators such as Med15B (Qi, Liu, Yu, Chen, & Liu, ), which are applied to manipulate gene transcription level.…”

Section: Discussionmentioning

confidence: 99%

“…To increase the tolerance of industrial strains to environmental stress (Chakraborty, Winardhi, Morgan, Yan, & Kenney, ; Olin‐Sandoval et al, ), a series of strategies have been developed. These include global transcription machinery engineering (Alper, Moxley, Nevoigt, Fink, & Stephanopoulos, ), adaptive laboratory evolution (Walker, Ryu, & Trinh, ), mutagenesis breeding (Cui et al, ), transporter engineering (Kell, Swainston, Pir, & Oliver, ), and membrane engineering (Tan et al, ).…”

Section: Introductionmentioning

confidence: 99%

“…(a) Engineering of membrane composition: the microbial cell membrane is composed of proteins, sugars, and primarily lipids (Sohlenkamp & Geiger, ). Therefore, engineering membrane composition has mainly focused on lipids to enhance microbial tolerance (Tan et al, ), production capacity (Tan, Yoon, Nielsen, Shanks, & Jarboe, ), and physiological function (Budin et al, ). (b) Engineering of membrane functions that include fluidity, integrity, and permeability (Qi, Liu, Chen, & Liu, ).…”

Section: Introductionmentioning

confidence: 99%

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Engineering of membrane phospholipid component enhances salt stress tolerance in Saccharomyces cerevisiae

Yin

Zhu

Luo

et al. 2020

Biotech & Bioengineering

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To increase the growth of industrial strains under environmental stress, the Saccharomyces cerevisiae BY4741 salt-tolerant strain Y00 that tolerates 1.2 M NaCl was cultured through nitroguanidine mutagenesis. The metabolomics and transcription data of Y00 were compared with those of the wild-type strain BY4741. The comparison identified two genes related to salt stress tolerance, cds1 and cho1. Modular assembly of cds1 and cho1 redistributed the membrane phospholipid component and decreased the ratio of anionic-to-zwitterionic phospholipid in strain Y03 that showed the highest salt tolerance. Therefore, significantly increased membrane potential and membrane integrity helped strain Y03 to resist salt stress (1.2 M NaCl). This study provides an effective membrane engineering strategy to enhance salt stress tolerance.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Engineering of membrane phospholipid component enhances salt stress tolerance in Saccharomyces cerevisiae

Yin

Zhu

Luo

et al. 2020

Biotech & Bioengineering

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“…Finally, modification of phospholipid head groups by overexpression of the phosphatidylserine synthase gene has also been found to be effective altering membrane hydrophilicity and integrity. In E. coli , this strategy proved effective for increasing tolerance towards a variety of lipophilic compounds, including aromatics such as styrene and toluene …”

Section: Enhancing Tolerance Towards Aromatic Biochemicalsmentioning

confidence: 99%

Emerging tools, enabling technologies, and future opportunities for the bioproduction of aromatic chemicals

Machas

Kurgan

Jha

et al. 2018

J of Chemical Tech & Biotech

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Aromatic compounds, which are traditionally derived from petroleum feedstocks, represent a diverse class of molecules with a wide range of industrial and commercial applications. Significant progress has been made to alternatively and sustainably produce many aromatics from renewable substrates using microbial biocatalysts. While the construction of both natural and non‐natural pathways has expanded the number and diversity of aromatic bioproducts, pathway modularization in both single‐ and multi‐strain systems continues to support the enhancement of key production metrics towards economically‐viable levels. Emerging tools for implementing more precise metabolic control (e.g. CRISPRi, sRNA) as well as the engineering of novel high‐throughput screening platforms utilizing in vivo aromatic biosensors, meanwhile, continue to facilitate further optimization of both pathways and hosts. While product toxicity persists as a key challenge limiting the production of many aromatics, various successful strategies have been demonstrated towards improving tolerance, including via membrane and efflux pump engineering as well as by exploiting alternative production hosts. Finally, as a further step towards sustainable and economical aromatic bioproduction, non‐model substrates including lignin‐derived compounds continue to emerge as viable feedstocks. This review highlights recent and notable achievements related to such efforts while offering future outlooks towards engineering microbial cell factories for aromatic production. © 2018 Society of Chemical Industry

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Strategies to improve the stress resistance of Escherichia coli in industrial biotechnology

Tao

Wang

et al. 2022

Biofuels Bioprod Bioref

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The use of industrial Escherichia coli strains for the production of bio‐based chemicals offers a promising and sustainable solution to a growing scarcity of non‐renewable resources and contributes to the protection of the environment. However, many limitations, such as inhibition from toxic substrates, hyperosmosis, toxic by‐products, and other adverse fermentation conditions, prevent strains from attaining a stable cell growth rate, efficient metabolic flux, and satisfactory productivity. There has thus been much effort to obtain E. coli strains that exhibit robust growth and productivity with enhanced tolerance of extreme operating conditions. In this review, we gained insights into recent studies in improving tolerance of E. coli and summarized four kinds of engineering methods: modifying regulation factors, regulating membrane structure, optimizing biosynthetic pathways, and applying adaptive evolution. The combination of different engineering strategies therefore provided a broader platform for robustness of industrial E. coli, making further steps to achieving more advantageous biosynthesis. © 2022 Society of Chemical Industry and John Wiley & Sons, Ltd

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Engineering Escherichia coli membrane phospholipid head distribution improves tolerance and production of biorenewables

Cited by 93 publications

References 70 publications

Engineering of membrane phospholipid component enhances salt stress tolerance in Saccharomyces cerevisiae

Engineering of membrane phospholipid component enhances salt stress tolerance in Saccharomyces cerevisiae

Emerging tools, enabling technologies, and future opportunities for the bioproduction of aromatic chemicals

Strategies to improve the stress resistance of Escherichia coli in industrial biotechnology

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