Engineering the cell–material interface for controlling stem cell adhesion, migration, and differentiation

Ayala, Ramsés; Zhang, Chao; Yang, Darren; Hwang, Yongsung; Aung, Aereas; Shroff, Sumeet S.; Arce, Fernando Terán; Lal, Ratnesh; Arya, Gaurav; Varghese, Shyni

doi:10.1016/j.biomaterials.2011.02.004

Cited by 299 publications

(264 citation statements)

References 53 publications

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“…On smaller grooves on the other hand, FA formation and maturation may occur perpendicular over several grooves, thereby initiating unidirectional migration (Hamilton et al 2010;Fujita et al, 2009). More importantly though, our cell migration and previously reported motility data (Lamers et al, 2010b) fully corroborate with the trend observed in initial cell adhesion, indicating that the initial contact of the cell with a specifi c surface characteristic may determine the cell fate at later time points (Ayala et al, 2011;Geiger et al, 2009). Our previous results on the infl uence of nanogrooved substrates on osteoblast differentiation confirm this, because differentiation increased with increasing groove pitch (Lamers et al, 2010a).…”

Section: Wwwecmjournalorg E Lamers Et Al Dynamic Cell Adhesion Andsupporting

confidence: 89%

Dynamic cell adhesion and migration on nanoscale grooved substrates

Lamers

Riet²,

Domański³

et al. 2012

eCM

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Organised nanotopography mimicking the natural extracellular matrix can be used to control morphology, cell motility, and differentiation. However, it is still unknown how specifi c cell types react with specifi c patterns. Both initial adhesion and preferential cell migration may be important to initiate and increase cell locomotion and coverage with cells, and thus achieve an enhanced wound healing response around an implantable material. Therefore, the aim of this study was to evaluate how MC3T3-E1 osteoblast initial adhesion and directional migration are infl uenced by nanogrooves with pitches ranging from 150 nm up to 1000 nm. In this study, we used a multipatterned substrate with fi ve different groove patterns and a smooth area with either a concentric or radial orientation. Initial cell adhesion measurements after 10 s were performed using atomic force spectroscopy-assisted single-cell force spectroscopy, and demonstrated that nascent cell adhesion was highly induced by a 600 nm pitch and reduced by a 150 nm pitch. Addition of RGD peptide signifi cantly reduced adhesion, indicating that integrins and cell adhesive proteins (e.g. fi bronectin or vitronectin) are key factors in specifi c cell adhesion on nanogrooved substrates. Also, cell migration was highly dependent on the groove pitch; the highest directional migration parallel to the grooves was observed on a 600 nm pitch, whereas a 150 nm pitch restrained directional cell migration. From this study, we conclude that grooves with a pitch of 600 nm may be favourable to enhance fast wound closure, thereby promoting tissue regeneration.

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Section: Wwwecmjournalorg E Lamers Et Al Dynamic Cell Adhesion Andsupporting

confidence: 89%

Dynamic cell adhesion and migration on nanoscale grooved substrates

Lamers

Riet²,

Domański³

et al. 2012

eCM

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“…Recent studies have demonstrated that material properties such as surface topography, 38 matrix stiffness, 39 hydrophobicity, 40 and surface chemistry 41 play an important role in directing the osteogenic differentiation of stem cells. Biomimetic mineralization approaches of assembling a nanostructured apatite layer throughout a three-dimensional porous bone tissue engineering scaffold, ie, biodegradable polymernanohydroxyapatite composites, have provided an effective tool for controlling surface chemistry and geometry within a large and complex structure, and may promote stem cells to differentiate towards osteoblasts.…”

Section: Discussionmentioning

confidence: 99%

Bone repair by periodontal ligament stem cell-seeded nanohydroxyapatite-chitosan scaffold

Ge¹,

Zhao²,

Wang³

et al. 2012

IJN

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Background: A nanohydroxyapatite-coated chitosan scaffold has been developed in recent years, but the effect of this composite scaffold on the viability and differentiation of periodontal ligament stem cells (PDLSCs) and bone repair is still unknown. This study explored the behavior of PDLSCs on a new nanohydroxyapatite-coated genipin-chitosan conjunction scaffold (HGCCS) in vitro as compared with an uncoated genipin-chitosan framework, and evaluated the effect of PDLSC-seeded HGCCS on bone repair in vivo. Methods: Human PDLSCs were cultured and identified, seeded on a HGCCS and on a genipinchitosan framework, and assessed by scanning electron microscopy, confocal laser scanning microscopy, MTT, alkaline phosphatase activity, and quantitative real-time polymerase chain reaction at different time intervals. Moreover, PDLSC-seeded scaffolds were used in a rat calvarial defect model, and new bone formation was assessed by hematoxylin and eosin staining at 12 weeks postoperatively. Results: PDLSCs were clonogenic and positive for STRO-1. They had the capacity to undergo osteogenic and adipogenic differentiation in vitro. When seeded on HGCCS, PDLSCs exhibited significantly greater viability, alkaline phosphatase activity, and upregulated the bone-related markers, bone sialoprotein, osteopontin, and osteocalcin to a greater extent compared with PDLSCs seeded on the genipin-chitosan framework. The use of PDLSC-seeded HGCCS promoted calvarial bone repair. Conclusion: This study demonstrates the potential of HGCCS combined with PDLSCs as a promising tool for bone regeneration.

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“…The monomers were synthesized and characterized as described elsewhere (18). See SI Text for more details.…”

Section: Methodsmentioning

confidence: 99%

“…We have previously shown that polymer hydrogels formulated from acryloyl-6-aminocaproic acid (A6ACA) precursors possess an optimal balance of hydrophobic and hydrophilic interactions that allows its side chains to bind to exogenous metal ions (2,11) and to extracellular proteins (18). The above finding suggests that the elastomeric properties of the A6ACA hydrogels along with their flexible side chains might be able to mediate hydrogen bonding across two hydrogel interfaces through the amide and carboxylic functional groups.…”

mentioning

confidence: 99%

Rapid self-healing hydrogels

Phadke

Zhang

Arman³

et al. 2012

Proc. Natl. Acad. Sci. U.S.A.

Self Cite

662

485

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Synthetic materials that are capable of autonomous healing upon damage are being developed at a rapid pace because of their many potential applications. Despite these advancements, achieving selfhealing in permanently cross-linked hydrogels has remained elusive because of the presence of water and irreversible cross-links. Here, we demonstrate that permanently cross-linked hydrogels can be engineered to exhibit self-healing in an aqueous environment. We achieve this feature by arming the hydrogel network with flexible-pendant side chains carrying an optimal balance of hydrophilic and hydrophobic moieties that allows the side chains to mediate hydrogen bonds across the hydrogel interfaces with minimal steric hindrance and hydrophobic collapse. The self-healing reported here is rapid, occurring within seconds of the insertion of a crack into the hydrogel or juxtaposition of two separate hydrogel pieces. The healing is reversible and can be switched on and off via changes in pH, allowing external control over the healing process. Moreover, the hydrogels can sustain multiple cycles of healing and separation without compromising their mechanical properties and healing kinetics. Beyond revealing how secondary interactions could be harnessed to introduce new functions to chemically crosslinked polymeric systems, we also demonstrate various potential applications of such easy-to-synthesize, smart, self-healing hydrogels.biomimetic materials | hydrophobicity | smart materials | molecular dynamics | adhesives R ecent years have witnessed an increasing interest in the development of "smart" materials that can sense changes in their environment and can accordingly adapt their properties and function, similar to living systems. Over the last decade, we have discovered and demonstrated a class of smart hydrogels that exhibit unique biomimicking functions: thermoresponsive volume phase transitions similar to sea cucumbers (1), self-organization into core-shell hollow structures similar to coconuts (2), shape memory as exhibited by living organisms (2), and metal ion-mediated cementing similar to marine mussels (3). A common thread connecting these smart hydrogels is their possession of a unique balance of hydrophilic and hydrophobic interactions that endows the hydrogels with the biomimicking properties described above. In this study, we demonstrate how this concept of balancing hydrophilic and hydrophobic forces could be exploited to design chemically cross-linked hydrogels with self-healing abilities.Indeed, materials capable of autonomous healing upon damage have numerous potential applications (4-6). So far, self-healing has been demonstrated in linear polymers (7), supramolecular networks (8, 9), dendrimer-clay systems (10), metal ion-polymer systems (11,12), and multicomponent systems (13-17). Whereas multicomponent thermosetting systems harness the ability of embedded chemical agents to repair cracks, supramolecular networks and noncovalent hydrogels employ secondary interactions such as hydrogen bonding, ionic interact...

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Engineering the cell–material interface for controlling stem cell adhesion, migration, and differentiation

Cited by 299 publications

References 53 publications

Dynamic cell adhesion and migration on nanoscale grooved substrates

Dynamic cell adhesion and migration on nanoscale grooved substrates

Bone repair by periodontal ligament stem cell-seeded nanohydroxyapatite-chitosan scaffold

Rapid self-healing hydrogels

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