Enhanced anti-proliferative and pro-apoptotic effects of metformin encapsulated PLGA-PEG nanoparticles on SKOV3 human ovarian carcinoma cells

Faramarzi, Leila; Dadashpour, Mehdi; Sadeghzadeh, Hadi; Mahdavi, Majid

doi:10.1080/21691401.2019.1573737

Cited by 40 publications

(37 citation statements)

References 43 publications

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“…NAR-NE produced the lowest cell viability (25.75 ± 1.75, 36.19 ± 1.18, and 44.62 ± 0.68%) at 100, 25, and 6.25 µg/mL, respectively, in comparison with NAR (37.21 ± 1.98, 46.44 ± 1.55, and 53.93 ± 1.69%). The greater potency of the naringenin nanoemulsion relative to free naringenin may reflect the higher release of naringenin from the nanoemulsion [ 26 , 40 ]. The difference in effectiveness between the nanoemulsion and free naringenin was much more marked at higher concentrations (100, 25, and 6.25 µg/mL).…”

Section: Resultsmentioning

confidence: 99%

“…Expression of Bax protein induces apoptosis, while expression of Bcl-2 enhances oncogenic/anti-apoptotic activities ( Figure 14 ) [ 40 , 42 ]. Treatment with NAR-NE produced a significant ( p < 0.05) increase in the expression of Bax (351.40 ± 6.31 pg/mL) compared with free NAR (206.20 ± 8.04 pg/mL); this may be explained by the enhancement of dissolution produced by the nanoemulsion.…”

Section: Resultsmentioning

confidence: 99%

“…Treatment with NAR-NE produced a significant ( p < 0.05) increase in the expression of Bax (351.40 ± 6.31 pg/mL) compared with free NAR (206.20 ± 8.04 pg/mL); this may be explained by the enhancement of dissolution produced by the nanoemulsion. On the other hand, treatment with NAR-NE produced a marked decrease in the expression of Bcl-2 compared with the control, with the effect of the nanoemulsion being greater than that of free naringenin [ 40 , 42 ].…”

Section: Resultsmentioning

confidence: 99%

“…The Annexin V-FITC (10 µL) and propidium iodide (PI) solution (5 µL) were added to the mixture and incubated for 5 min (25 °C). The analysis was carried out using a flow-cytometer (FACS Calibur, BD Bioscience, CA, USA) [ 40 , 42 , 43 ].…”

Section: Methodsmentioning

confidence: 99%

“…The pellet was rinsed with PBS, fixed in 70% ethanol, and then kept at −20 °C overnight. Before analysis, the cells were rinsed with PBS to remove the alcohol and stained with PI and RNAase as per the manufacturer’s instructions and analyzed using flow cytometry [ 40 , 42 , 43 ].…”

Section: Methodsmentioning

confidence: 99%

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Formulation Design, Statistical Optimization, and In Vitro Evaluation of a Naringenin Nanoemulsion to Enhance Apoptotic Activity in A549 Lung Cancer Cells

et al. 2020

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Naringenin (NAR), a flavonoid mainly found in citrus and grapefruits, has proven anti-cancer activities. However, the poor water solubility and low bioavailability of NAR limits its use as a therapeutic agent. The aim of this study was to develop and optimize stable naringenin nanoemulsions (NAR-NE) using a Box–Behnken experimental design to obtain a formulation with a higher efficiency. Anticancer activity of optimized NAR-NE was evaluated in A549 lung cancer cells using cell viability, flow-cytometric assays, and enzyme-linked immunosorbent assay. The stabilized nanoemulsion, which showed a spherical surface morphology, had a globule size of 85.6 ± 2.1 nm, a polydispersity index of 0.263 ± 0.02, a zeta potential of −9.6 ± 1.2 mV, and a drug content of 97.34 ± 1.3%. The NAR release from the nanoemulsion showed an initial burst release followed by a stable and controlled release for a longer period of 24 h. The nanoemulsion exhibited excellent thermodynamic and physical stability against phase separation and storage. The NAR-NE showed concentration-dependent cytotoxicity in A549 lung cancer cells, which was greater than that of free NAR. The percentage of apoptotic cells and cell cycle arrest at the G2/M and pre-G1 phases induced by NAR-NE were significantly higher than those produced by free NAR (p < 0.05). NAR-NEs were more effective than the NAR solution in reducing Bcl2 expression, while increasing pro-apoptotic Bax and caspase-3 activity. Therefore, stabilized NAR-NE could be a suitable drug delivery system to enhance the effects of NAR in the treatment of lung cancer.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Formulation Design, Statistical Optimization, and In Vitro Evaluation of a Naringenin Nanoemulsion to Enhance Apoptotic Activity in A549 Lung Cancer Cells

et al. 2020

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Mass Spectrometry‐based Mitochondrial Proteomics in Human Ovarian Cancers

Zhan

2020

Mass Spectrometry Reviews

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The prominent characteristics of mitochondria are highly dynamic and regulatory, which have crucial roles in cell metabolism, biosynthetic, senescence, apoptosis, and signaling pathways. Mitochondrial dysfunction might lead to multiple serious diseases, including cancer. Therefore, identification of mitochondrial proteins in cancer could provide a global view of tumorigenesis and progression. Mass spectrometry‐based quantitative mitochondrial proteomics fulfils this task by enabling systems‐wide, accurate, and quantitative analysis of mitochondrial protein abundance, and mitochondrial protein posttranslational modifications (PTMs). Multiple quantitative proteomics techniques, including isotope‐coded affinity tag, stable isotope labeling with amino acids in cell culture, isobaric tags for relative and absolute quantification, tandem mass tags, and label‐free quantification, in combination with different PTM‐peptide enrichment methods such as TiO2 enrichment of tryptic phosphopeptides and antibody enrichment of other PTM‐peptides, increase flexibility for researchers to study mitochondrial proteomes. This article reviews isolation and purification of mitochondria, quantitative mitochondrial proteomics, quantitative mitochondrial phosphoproteomics, mitochondrial protein‐involved signaling pathway networks, mitochondrial phosphoprotein‐involved signaling pathway networks, integration of mitochondrial proteomic and phosphoproteomic data with whole tissue proteomic and transcriptomic data and clinical information in ovarian cancers (OC) to in‐depth understand its molecular mechanisms, and discover effective mitochondrial biomarkers and therapeutic targets for predictive, preventive, and personalized treatment of OC. This proof‐of‐principle model about OC mitochondrial proteomics is easily implementable to other cancer types. © 2020 John Wiley & Sons Ltd. Mass Spec Rev

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Nanocargos designed with synthetic and natural polymers for ovarian cancer management

Ps¹,

Guha²,

Balasubramanian³

et al. 2023

Naunyn-Schmiedeberg's Arch Pharmacol

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Enhanced anti-proliferative and pro-apoptotic effects of metformin encapsulated PLGA-PEG nanoparticles on SKOV3 human ovarian carcinoma cells

Cited by 40 publications

References 43 publications

Formulation Design, Statistical Optimization, and In Vitro Evaluation of a Naringenin Nanoemulsion to Enhance Apoptotic Activity in A549 Lung Cancer Cells

Formulation Design, Statistical Optimization, and In Vitro Evaluation of a Naringenin Nanoemulsion to Enhance Apoptotic Activity in A549 Lung Cancer Cells

Mass Spectrometry‐based Mitochondrial Proteomics in Human Ovarian Cancers

Nanocargos designed with synthetic and natural polymers for ovarian cancer management

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