Enhanced cardiomyogenic lineage differentiation of adult bone-marrow-derived stem cells grown on cardiogel

Sreejit, P.; Verma, Rama Shanker

doi:10.1007/s00441-013-1661-3

Cited by 20 publications

(12 citation statements)

References 80 publications

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“…Compared to natural scaffolds which are derived from destroyed ECM, decellularized ECM‐derived scaffolds are advantageous to preserve the complex ECM architecture and thus to maintain cell‐matrix interactions, which are uneasy to achieve by synthetic polymer scaffolds . For example, Verma and co‐workers developed several ECM‐derived scaffolds, termed as ‘cardiogel’, to repair infarcted myocardial tissues . Cardiogel promoted proliferation, adhesion and migration of BMSCs while aiding cardiomyogenic differentiation and angiogenesis .…”

Section: Type Of Polymers and Design Strategiesmentioning

confidence: 99%

“…[26,27] For example, Verma and co-workers developed several ECM-derived scaffolds, termed as 'cardiogel', to repair infarcted myocardial tissues. [28][29][30] Cardiogel promoted proliferation, adhesion and migration of BMSCs while aiding cardiomyogenic differentiation and angiogenesis. [30] ADSCs embedded in decellularized adipose tissue (DAT)-derived ECM scaffold accelerated in vivo fat regeneration through enhanced adipogenesis and angiogenesis.…”

Section: Biodegradable Scaffold To Promote Angiogenesismentioning

confidence: 99%

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Design of Polymeric Culture Substrates to Promote Proangiogenic Potential of Stem Cells

Kwon

Wang

Kang

et al. 2017

Macromolecular Bioscience

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Stem cells are a promising cell source for regenerative medicine due to their differentiation and self-renewal capacities. In the field of regenerative medicine and tissue engineering, a variety of biomedical technologies have been tested to improve proangiogenic activities of stem cells. However, their therapeutic effect is found to be limited in the clinic because of cell loss, senescence, and insufficient therapeutic activities. To address this type of issue, advanced techniques for biomaterial synthesis and fabrication have been approached to mimic proangiogenic microenvironment and to direct proangiogenic activities. This review highlights the types of polymers and design strategies that have been studied to promote proangiogenic activities of stem cells. In particular, scaffolds, hydrogels, and surface topographies, as well as insight into their underlying mechanisms to improve proangiogenic activities are the focuses. The strategy to promote angiogenic activities of hMSCs by controlling substrate repellency is introduced, and the future direction is proposed.

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Section: Type Of Polymers and Design Strategiesmentioning

confidence: 99%

Section: Biodegradable Scaffold To Promote Angiogenesismentioning

confidence: 99%

Design of Polymeric Culture Substrates to Promote Proangiogenic Potential of Stem Cells

Kwon

Wang

Kang

et al. 2017

Macromolecular Bioscience

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“…In many cases, the outcome is the formation of brocartilaginous repair tissue that does not possess the full load-bearing properties and durability of healthy articular cartilage. 7,8 Their harvesting methods in humans are minimally invasive, and they proliferate more readily ex vivo than osteoblasts. The signicance of cartilage injury requires novel cartilage tissue engineering strategies.…”

Section: Introductionmentioning

confidence: 99%

Fabrication and in vivo chondrification of a poly(propylene carbonate)/l-lactide-grafted tetracalcium phosphate electrospun scaffold for cartilage tissue engineering

et al. 2015

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Regenerative therapies that utilize stem cell differentiation in three-dimensional porous scaffolds have attracted significant interest in recent years. In this study, fibrous poly(propylene carbonate)/poly(L-lactic acid)-grafted tetracalcium phosphate (PPC/g-TTCP) scaffolds were prepared using an electrospinning method. The characteristics of the fabricated scaffolds were investigated using scanning electron microscopy, differential scanning calorimetry, thermogravimetric analyses, Fourier transform infrared spectroscopy, X-ray diffraction analyses, water contact angle measurements and tensile tests. Due to the importance of biocompatibility, rat bone marrow-derived stem cells were cultured on the scaffolds, and the cell proliferation was investigated using 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide assays. Subsequently, chondrogenic differentiation was induced in these cells in vitro and in vivo. Fourteen days later, chondrocyte-like cells had developed on the PPC/g-TTCP scaffolds, as evidenced by the accumulation of glycosaminoglycan and type II collagen. After subcutaneous transplantation into nude mice, a typical cartilage cell morphology was observed on the scaffolds. These findings suggest that PPC/g-TTCP scaffolds can support cartilage development and are excellent candidate scaffolds for cartilage defect repair.

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“…Cardiogel has been known to improve cardiomyocyte growth and maturation. Bone Marrow derived Stromal/Stem Cells (BMSCs) cultured on their own secreted ECM do not demonstrate protection against oxidative stress or cardiomyogenic differentiation; but BMSCs cultured on cardiogel showed increased cell proliferation and adhesion, enhanced cardiomyogenic differentiation and protection against oxidative stress [12] – [17] . However, the ECM components that contribute to the biological properties of cardiogel have not yet been completely characterized.…”

Section: Introductionmentioning

confidence: 99%

“…Comparative proteomic analysis using nano-liquid chromatography tandem-mass spectrometry (nLC-MS/MS) analysis with mesogel as control was used to identify unique ECM components of cardiogel, which may explain cardiogel's biological properties such as heightened protection against oxidative stress and enhanced cardiomyogenic differentiation [16] , [17] . Furthermore, biological properties of cardiogel such as the cytocompatibility, potential for cardiomyogenic differentiation and angiogenesis were evaluated to validate cardiogel as a potential scaffold for cardiac regeneration.…”

Section: Introductionmentioning

confidence: 99%

Cardiogel: A Nano-Matrix Scaffold with Potential Application in Cardiac Regeneration Using Mesenchymal Stem Cells

2014

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3-Dimensional conditions for the culture of Bone Marrow-derived Stromal/Stem Cells (BMSCs) can be generated with scaffolds of biological origin. Cardiogel, a cardiac fibroblast-derived Extracellular Matrix (ECM) has been previously shown to promote cardiomyogenic differentiation of BMSCs and provide protection against oxidative stress. To determine the matrix composition and identify significant proteins in cardiogel, we investigated the differences in the composition of this nanomatrix and a BMSC-derived ECM scaffold, termed as ‘mesogel’. An optimized protocol was developed that resulted in efficient decellularization while providing the maximum yield of ECM. The proteins were sequentially solubilized using acetic acid, Sodium Dodecyl Sulfate (SDS) and Dithiothreitol (DTT). These proteins were then analyzed using surfactant-assisted in-solution digestion followed by nano-liquid chromatography and tandem mass spectrometry (nLC-MS/MS). The results of these analyses revealed significant differences in their respective compositions and 17 significant ECM/matricellular proteins were differentially identified between cardiogel and mesogel. We observed that cardiogel also promoted cell proliferation, adhesion and migration while enhancing cardiomyogenic differentiation and angiogenesis. In conclusion, we developed a reproducible method for efficient extraction and solubilization of in vitro cultured cell-derived extracellular matrix. We report several important proteins differentially identified between cardiogel and mesogel, which can explain the biological properties of cardiogel. We also demonstrated the cardiomyogenic differentiation and angiogenic potential of cardiogel even in the absence of any external growth factors. The transplantation of Bone Marrow derived Stromal/Stem Cells (BMSCs) cultured on such a nanomatrix has potential applications in regenerative therapy for Myocardial Infarction (MI).

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Enhanced cardiomyogenic lineage differentiation of adult bone-marrow-derived stem cells grown on cardiogel

Cited by 20 publications

References 80 publications

Design of Polymeric Culture Substrates to Promote Proangiogenic Potential of Stem Cells

Design of Polymeric Culture Substrates to Promote Proangiogenic Potential of Stem Cells

Fabrication and in vivo chondrification of a poly(propylene carbonate)/l-lactide-grafted tetracalcium phosphate electrospun scaffold for cartilage tissue engineering

Cardiogel: A Nano-Matrix Scaffold with Potential Application in Cardiac Regeneration Using Mesenchymal Stem Cells

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