Enhanced decellularization technique of porcine dermal ECM for tissue engineering applications

Ventura, Reiza D.; Padalhin, Andrew R.; Park, Chan Mi; Lee, Byong‐Taek

doi:10.1016/j.msec.2019.109841

Cited by 63 publications

(39 citation statements)

References 48 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Many methods have been examined for performing decellularization, including physical, chemical and enzymatic approaches, and generally multiple methods are combined for effective decellularization [7]. Skin tends to be more difficult to decellularize than other tissues due to its dense, complex structure and abundant lipid content [8]. In the decellularization process, trypsin is used as an enzymatic agent, cleaving peptide bonds on the C-side of Arg and Lys, effectively removing cellular components from ECM.…”

Section: Introductionmentioning

confidence: 99%

“…dECM has been shown to promote the in vivo creation of site-specific, functional tissue [8,9,12], making dECM highly desirable for tissue regeneration and wound healing applications. dECM has previously been processed into many different delivery forms, including powders, coatings, injectable hydrogels and hydrogels [8], and have been isolated from multiple organ systems, including skin, heart, liver, nerves, tendon, and blood vessel have been used in various tissue engineering applications [8]. While collagen is the major component of human skin ECM, other human skin ECM proteins may foster additional cellular attachment and activities [13].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Decellularized Skin Extracellular Matrix (dsECM) Improves the Physical and Biological Properties of Fibrinogen Hydrogel for Skin Bioprinting Applications

et al. 2020

View full text Add to dashboard Cite

Full-thickness skin wounds are a significant clinical burden in the United States. Skin bioprinting is a relatively new technology that is under investigation as a new treatment for full-thickness injuries, and development of hydrogels with strong physical and biological characteristics are required to improve both structural integrity of the printed constructs while allowing for a more normal extracellular matrix milieu. This project aims to evaluate the physical and biological characteristics of fibrinogen hydrogel supplemented with decellularized human skin-derived extracellular matrix (dsECM). The hybrid hydrogel improves the cell viability and structural strength of bioprinted skin constructs. Scanning electron microscopy demonstrates that the hybrid hydrogel is composed of both swelling bundles interlocked in a fibrin network, similar to healthy human skin. This hybrid hydrogel has improved rheological properties and shear thinning properties. Extrusion-based printing of the fibrinogen hydrogel + dsECM demonstrates significant improvement in crosshatch pore size. These findings suggest that incorporating the properties of dsECM and fibrinogen hydrogels will improve in vivo integration of the bioprinted skin constructs and support of healthy skin wound regeneration.

show abstract

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Decellularized Skin Extracellular Matrix (dsECM) Improves the Physical and Biological Properties of Fibrinogen Hydrogel for Skin Bioprinting Applications

et al. 2020

View full text Add to dashboard Cite

show abstract

“…in addition, HoXa11 expression was indicated to influence TGF-β1 expression, and vice versa. l929 murine fibroblasts have been extensively used to assess the ECM (37). In order to further investigate the function of HoXa11 and TGF-β1, the expression levels of HoXa11 and TGF-β1 were knocked down.…”

Section: Discussionmentioning

confidence: 99%

Molecular mechanism of extracellular matrix disorder in pelvic organ prolapses

Zhang

Dai

Chen³

et al. 2020

Mol Med Rep

View full text Add to dashboard Cite

Pelvic organ prolapses (PoP) notably reduces the quality of life in elderly populations due to bladder and bowel dysfunction, incontinence, and coital problems. extracellular matrix (ecM) disorder is a pivotal event in the progression of PoP, but to date, its specific underlying mechanism remains unclear. The ligaments of patients with PoP and healthy controls were collected to compare the expression of Homeobox11 (HoXa11) and transforming growth factor β (TGF-β1) via immunohistochemical analysis. HoXa11 and TGF-β1 were overexpressed or knocked down in fibroblast cells to explore their effects on the expression of collagen and matrix metalloproteinases (MMPs). HoXa11 and TGF-β1 were greatly reduced in the ligaments of patients with PoP. The overexpression and downregulation of HoXa11 and TGF-β1 can mediate ecM disorder via regulating expression of collagen (col) and MMPs. in addition, HoXa11 and TGF-β1 exerted synergistic effect on the expression of col and MMPs. The present study identified that HOXA11 and TGF-β1 serve critical roles in mediating ecM disorders, which may be of clinical significance for the diagnosis and treatment of patients with PoP.

show abstract

“…In all groups, absorption bands indicating C=O and N–H stretching of peptides were observed for the amide A (3307 cm −1 ) and amide B (2927 cm −1 ) peaks, respectively. 23 , 24 Amide I (1654 cm −1 ), amide II (1548cm −1 ), and amide III (1238cm −1 )—referred to as the collagen fingerprint—and glycosaminoglycan (1048 cm −1 ) peaks were also observed. 25 , 26 TXA-dECM bio-inks had the largest peaks, and the intensities decreased in the order TXA- > SDC- > SDS-dECM bio-inks.…”

Section: Resultsmentioning

confidence: 99%

Effect of detergent type on the performance of liver decellularized extracellular matrix-based bio-inks

2021

View full text Add to dashboard Cite

Decellularized extracellular matrix-based bio-inks (dECM bio-inks) for bioprinting technology have recently gained attention owing to their excellent ability to confer tissue-specific functions and 3D-printing capability. Although decellularization has led to a major advancement in bio-ink development, the effects of detergent type, the most important factor in decellularization, are still unclear. In this study, the effects of various detergent types on bio-ink performance were investigated. Porcine liver-derived dECM bio-inks prepared using widely used detergents, including sodium dodecyl sulfate (SDS), sodium deoxycholate (SDC), Triton X-100 (TX), and TX with ammonium hydroxide (TXA), were characterized in detail. SDS and SDC severely damaged glycosaminoglycan and elastin proteins, TX showed the lowest rate of decellularization, and TXA-based dECM bio-ink possessed the highest ECM content among all bio-inks. Differences in biochemical composition directly affected bio-ink performance, with TXA-dECM bio-ink showing the best performance with respect to gelation kinetics, intermolecular bonding, mechanical properties, and 2D/3D printability. More importantly, cytocompatibility tests using primary mouse hepatocytes also showed that the TXA-dECM bio-ink improved albumin secretion and cytochrome P450 activity by approximately 2.12- and 1.67-fold, respectively, compared with the observed values for other bio-inks. Our results indicate that the detergent type has a great influence on dECM damage and that the higher the dECM content, the better the performance of the bio-ink for 3D bioprinting.

show abstract

Enhanced decellularization technique of porcine dermal ECM for tissue engineering applications

Cited by 63 publications

References 48 publications

Decellularized Skin Extracellular Matrix (dsECM) Improves the Physical and Biological Properties of Fibrinogen Hydrogel for Skin Bioprinting Applications

Decellularized Skin Extracellular Matrix (dsECM) Improves the Physical and Biological Properties of Fibrinogen Hydrogel for Skin Bioprinting Applications

Molecular mechanism of extracellular matrix disorder in pelvic organ prolapses

Effect of detergent type on the performance of liver decellularized extracellular matrix-based bio-inks

Contact Info

Product

Resources

About