Enhanced HPC recruitment in children using LVL and a new automated apheresis system

Torrabadella, M; Olivé, T; Ortega, J.J.; Massuet, L

doi:10.1046/j.1537-2995.2000.40040404.x

Cited by 19 publications

(14 citation statements)

References 22 publications

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“…The recruitment was limited to MNC and CD34+ cells, which were predominantly removed during the procedure, which was also observed by other authors [8,23,24,43]. Interestingly, recruitment factor for CD34+ cells in our study was significantly higher in poor mobilizers than in good mobilizers, which points to the importance of LVL in patients who mobilize low number of CD34+ cells [17]. The underlying mechanisms of PBSCs recruitment during LVL are not clear, and few hypotheses have been proposed.…”

Section: Bojanic 17supporting

confidence: 82%

“…Results of previous studies which compared standard vs. LVL processing varied considerably [2,4,10,[14][15][16][17][18]. It is difficult to establish a control group with comparable patients due to high interindividual variability regarding patients' characteristics, such as age, gender, TBV, stage of disease, previous treatment, and baseline blood count values.…”

Section: Discussionmentioning

confidence: 99%

“…tolerance, and consistence with working hours of apheresis unit, quality control and cell processing laboratories. Time-consuming LVL raises an issue of patient's compliance, but previous studies showed that cooperation for prolonged apheresis procedure could be achieved even in pediatric patients [17,25,26].…”

Section: Discussionmentioning

confidence: 99%

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Large volume leukapheresis: Efficacy and safety of processing patient’s total blood volume six times

Bojanić

Dubravčić

Batinić

et al. 2011

Transfusion and Apheresis Science

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Section: Bojanic 17supporting

confidence: 82%

Section: Discussionmentioning

confidence: 99%

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Large volume leukapheresis: Efficacy and safety of processing patient’s total blood volume six times

Bojanić

Dubravčić

Batinić

et al. 2011

Transfusion and Apheresis Science

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“…6 Two million CD34 + cells/kg body weight (BW) was established as the minimum CD34 + cell dose required, and 5 ϫ 10 6 CD34 + cells/kg BW was considered to be an optimum collection. 5 Collection efficiency (CE) was calculated according to the Ford et al formula, 8 and recruitment according to Torrabadella et al 9 A software program (Statview 4.0; Abacus Concept, Berkeley, CA, USA) was used for statistical analysis. Correlations were determined using linear regression.…”

Section: Methodsmentioning

confidence: 99%

Granulocyte colony-stimulating factor alone at 12 μg/kg twice a day for 4 days for peripheral blood progenitor cell priming in pediatric patients

Sevilla

González‐Vicent

Madero

et al. 2002

Bone Marrow Transplant

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Summary:In children, the optimal mobilization schedule for harvesting peripheral blood progenitor cells (PBPC) is an issue of continuous research. We have studied a schedule based on high and daily divided doses of G-CSF (12 g/kg body weight twice daily) for 4 days for PBPC priming. Toxicity related to G-CSF was observed in 13 patients (23%), mainly mild bone pain and myalgia. The median CD34 + cell number collected was 4.4 (0.4-35 ؋ 10 6 /kg body weight), with 46 patients achieving 2 ؋ 10 6 /kg body weight (83.6%) after a single large volume leukapheresis. In conclusion, this mobilization schedule allows safe and efficient collection of the minimum target CD34 + cell dose in most pediatric patients by only one procedure. Bone Marrow Transplantation (2002) 30, 417-420. doi:10.1038/sj.bmt.1703662 Keywords: G-CSF; priming; LVL; children; mobilization In children, peripheral blood progenitor cells (PBPC) are progressively substituting bone marrow as a source of stem cells for hematopoietic transplantation due to their easier collection and faster engraftment. The optimal mobilization schedule for stem cell harvest is a matter of permanent research, since collecting the maximum dose of CD34 + cells is being proposed in order to facilitate subsequent manipulation and shorten granulocyte and platelet engraftment. Although chemotherapy plus growth factors has been reported repeatedly to result in collecting higher numbers of CD34 + cells than G-CSF alone, it has several disadvantages such as a longer mobilization period, chemotherapy toxicity and febrile neutropenia. Moreover, optimal timing of apheresis is not easily scheduled and this hinders optimal progenitor collection especially in patients referred from other regions.In order to improve the progenitor cell collection in our

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“…Collection using semi-automated methods is influenced by an operator technique increasing the work load for dedicated personnel. Automated methods, on the other hand, offer the advantage of improved standardization along with a decreased work load [9][10][11]. Automated methods, which produce a leukapheresis product with a high stem cell content, have the potential to reduce the number of procedures, to reduce procedure-related risk [12,13], and to facilitate further manipulation of the graft (e.g., immunomagnetic cell selection and tumor purging) [14].…”

Section: Introductionmentioning

confidence: 99%

Clinical impact of a new automated system employed for peripheral blood stem cell collection

et al. 2006

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At the moment, PBSC collections can be performed using semi-automated or automated cell separator devices. The automated methods offer the advantages of a decreased working load for dedicated personnel and high standardization of the collection procedure. Herein we report our single institutional experience in 80 PBSC collections employing the new automated COM.TEC Fresenius autoMNC program that provides the ability to predict the total number of CD34(+) cells collected, based on the pre-leukapheresis CD34(+) cell count in peripheral blood. Fourty-eight patients and 21 healthy donors were mobilized with chemotherapy + G-CSF or G-CSF alone, respectively. Eighty leukapheresis collections were performed starting with a CD34(+) cell count in peripheral blood at least of 20/microL. Collection parameters and related side effects were evaluated. The mean CD34(+) cell collection efficiency in patients and donors was 81.8% (sd 27.6) and 95.1% (sd 15.6), respectively. The mean difference between real and predicted CD34(+) cells was +30.2% (sd 92.9) for patients and +4.6% (sd 30.3) for donors. The mean leukapheresis bag volume was 240.7 ml (sd 67.3) and 310.3 ml (sd 86.8) with a mean HCT of 10.9% (sd 34.4) and 9.2% (sd 3.9) for patients and donors, respectively. The automated PBSC collection with the new program COM.TEC Fresenius autoMNC demonstrated a very high CD34(+) cell collection efficiency. Moreover, the ability to predict the CD34(+) cell yield permits improved management of the leukapheresis collection, with the only disadvantage of larger leukapheresis volume and higher hematocrit.

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Enhanced HPC recruitment in children using LVL and a new automated apheresis system

Cited by 19 publications

References 22 publications

Large volume leukapheresis: Efficacy and safety of processing patient’s total blood volume six times

Large volume leukapheresis: Efficacy and safety of processing patient’s total blood volume six times

Granulocyte colony-stimulating factor alone at 12 μg/kg twice a day for 4 days for peripheral blood progenitor cell priming in pediatric patients

Clinical impact of a new automated system employed for peripheral blood stem cell collection

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