Clinical impact of a new automated system employed for peripheral blood stem cell collection

Abstract

At the moment, PBSC collections can be performed using semi-automated or automated cell separator devices. The automated methods offer the advantages of a decreased working load for dedicated personnel and high standardization of the collection procedure. Herein we report our single institutional experience in 80 PBSC collections employing the new automated COM.TEC Fresenius autoMNC program that provides the ability to predict the total number of CD34(+) cells collected, based on the pre-leukapheresis CD34(+) cell count in peripheral blood. Fourty-eight patients and 21 healthy donors were mobilized with chemotherapy + G-CSF or G-CSF alone, respectively. Eighty leukapheresis collections were performed starting with a CD34(+) cell count in peripheral blood at least of 20/microL. Collection parameters and related side effects were evaluated. The mean CD34(+) cell collection efficiency in patients and donors was 81.8% (sd 27.6) and 95.1% (sd 15.6), respectively. The mean difference between real and predicted CD34(+) cells was +30.2% (sd 92.9) for patients and +4.6% (sd 30.3) for donors. The mean leukapheresis bag volume was 240.7 ml (sd 67.3) and 310.3 ml (sd 86.8) with a mean HCT of 10.9% (sd 34.4) and 9.2% (sd 3.9) for patients and donors, respectively. The automated PBSC collection with the new program COM.TEC Fresenius autoMNC demonstrated a very high CD34(+) cell collection efficiency. Moreover, the ability to predict the CD34(+) cell yield permits improved management of the leukapheresis collection, with the only disadvantage of larger leukapheresis volume and higher hematocrit.

Mononuclear cell collection for extracorporeal photopheresis by using the “off‐line” system: A comparative study between COBE Spectra and Spectra Optia devices

“…In our study, even if higher in the Spectra Optia group, product Hct was constantly below 3% and did not affect the illumination time This is also in line with findings reported by Piccirillo et al A recent study reported a higher product Hct with the MNC protocol compared to ghe CMNC program This is consistent with our finding, as most of the Spectra Optia procedures (67%) in our study were run with the MNC protocol. The higher product Hct observed in the Spectra Optia group may also reflect collections deeper in the MNC layer, explaining also a part of the higher MNC collection efficiencies as well as the higher granulocyte contamination.…”

Impact of Hematopoietic Progenitor Cell Count as an Indicator for Optimal Timing of Peripheral Stem Cell Harvest in Clinical Practice

“…The second reason is more likely because the myeloblast count in the PB on the harvest day was zero in all the healthy donors except for one. Previous reports revealed a correlation between hematocrit and CD34+ cells in the PBSC yield in healthy donors [14,15]. Among the healthy donors in our study, however, there was no correlation between hemoglobin on the harvest day and the CD34+ cell count in the PBSC yields (data not shown).…”

Automated CD14+ monocyte collection with the autoMNC program of the COM.TEC cell separator

“…Del Fante and coworkers 6 found a significant correlation between donor predonation CD34‐cell counts and yields in the products by use of the new autoMNC program. Our study found that predonation WBC counts correlated well with monocyte yields.…”

“…Further, it was observed that increase of RBCs in original MNC harvests reduced monocyte yields after counterflow elutriation 12 . Del Fante and coworkers 6 described two disadvantages of the autoMNC program, the collection of large BC volumes and high yields of RBCs. Our data showed that the standard MNC program collected significantly more RBCs compared to the autoMNC program (Table 1).…”

“…The standard MNC program of the COM.TEC cell separator, however, also collected large yields of residual platelets (PLTs) and red blood cells (RBCs) 1 . Del Fante and coworkers 6 reported that the autoMNC program produced large buffy‐coat (BC) volume with high RBC contamination. The contamination of WBC harvests with residual cells may also cause cell loss in blood donors.…”