Enhanced Silymarin accumulation is related to calcium deprivation in cell suspension cultures of Silybum marianum (L.) Gaertn

Evaluation of the yeast-extract signaling pathway leading to silymarin biosynthesis in milk thistle hairy root culture

“…Sanchez-Sampedro et al (2005) and Hasanloo et al (2008) reported that the treatment of S. marianum cell cultures with methyl jasmonate strongly increases silymarin accumulation both in cells and in the culture medium. Addition of YE to S. marianum cultures also improved the production of silymarin to a level about three-fold higher than that of the control (Sánchez-Sampedro et al 2005). They proposed that YE may cause a complex stress response in the cultures in addition to creating an accumulation of flavonolignans.…”

Enhanced production of silymarin by Ag+elicitor in cell suspension cultures ofSilybum marianum

“…A number of elicitors, such as yeast extract, chitin, chitosan, and methyl jasmonate, have been investigated for the enhancement of flavonolignan production in cell cultures of S. marianum (Sanchez-Sampedro et al, 2005b). Results showed that yeast extract stimulated production.…”

Enhanced resveratrol accumulation in rolB transgenic cultures of Vitis amurensis correlates with unusual changes in CDPK gene expression

“…Inhibition of Ca 2+ channels, calcium chelating or blocking of calcium intracellular fluxes, by means of special agents, considerably reduced phytoalexin accumulation. However, contradictory reports have also appeared in the literature, indicating that calcium negatively affected the production of some secondary metabolites (Cacho et al, 1995;Ning et al, 1998;Sanchez-Sampedro et al, 2005).…”