Ethanol Extract of Miana Leaf (Coleus atropurpureus Benth) As Analgetic Antiinflamation in Rats (Rattus novergicus)

Abstract

Background: Inflammation is a normal protective response to tissue injury caused by physical trauma, chemicals, or microbiological substances. The manifestation clinic of inflammation is pain. Miana leaves (Coleus atropurpureus Benth.) have chemical contents such as essential oils, phenols, flavonoids, and polyphenols. Flavonoids can protect lipid membranes from damage and inhibit cyclooxygenase, which is the first pathway for the synthesis of pain mediators such as prostaglandins. Objectives: This study aims to determine the effectiveness of ethanol extract of Miana leaves (Coleus atropurpureus Benth) (EEML) as analgesic and anti-inflammatory in rats (Rattus novergicus). Material and Methods: This study used The Pre-Posttest-Only Control Group Design, twenty male rats were divided into 5 groups namely negative control, positive control was given diclofenac sodium, treatment with EEML dose of 150 mg/kgBW, 300 mg/kgBW, and 600 mg/kgBW. Test of Antiinflammatory uses the Rat Hind Paw Edema method where the condition of inflammation in rats is induced with 5% egg white by subplantar. The measurement of edema in rat feet used a caliper every 30 minutes for 5 hours. While The measurement of analgesic testing used the method of nociception test which evaluates pain responses such as Staggering Gait, Vocalization, and Writhing. Results: The results of the measurement of the inflammatory parameters were analyzed with the One Way Anova test for the width of edema foot and Kruskal Walis for the thickness of edema foot. While the results of observing the pain response were analyzed with the Kruskal Walis test. Conclusions: This study concluded that the ethanol extract of Miana leaves has anti-inflammatory analgesic especially at a dose of 600 mg/kg BW which results are non-significant (p> 0.05) with a positive control group (sodium diclofenac).

A potential mechanism of miana (Coleus scutellariodes) and quercetin via NF-κB in Salmonella typhi infection

“…NF-κB can be activated in the presence of oxidation stress which will induce Reactive Oxygen Species (ROS) and affect the activation of NF-κB and HIF-1α in hypoxic conditions due to microorganism infection and doxorubicin induction in cardiac failure [ 12 , 28 , 29 ]. NF-κB plays a role in bacterial infection, where bacteria or bacterial components can activate NF-κB and signaling molecules at the initial of infection and then NF-κB will strengthen and ensure that the inflammatory process runs continuously resulting in pro-inflammatory cytokines and tissue hypoxia [ 30 , 31 ].…”

“…In previous studies it was proved that giving Miana which contains flavonoids and Quercetin which is a derivative of flavonoids has an anti-inflammatory effects because it can inhibit the release of histamine, inflammatory cytokines. Inhibits leukocyte metabolic activity, and inflammatory cytokines release [ [12] , [13] , [14] , [15] ] The anti-inflammatory effect of Quercetin and Miana can be mediated by inhibition of NF-κB activation and inflammatory cytokine release [ 16 ]. Miana also significantly reduced the levels of Vascular Endothelial Growth Factor (VEGF), Hypoxia Inducible Factor-1 alpha (HIF-1α), and reduced inflammation in M. tuberculosis infection [ 17 , 18 ].…”

Teknik Budidaya Iler (Coleus atropurpureus. L. Benth) sebagai Herba untuk Penanganan Gejala (Demam dan Batuk) Penyintas Covid-19

“…Hasil penelitian dibidang biokimia kesehatan dan farmakologi melaporkan bahwa tanaman ini berkhasiat menekan inflamasi (antiinflamasi) dan bekerja secara analgesik pada tubuh untuk menurunkan tingkat keparahan rasa sakit (Verawati et al, 2016;Pakadang & Salim, 2019;Sukmawati et al, 2022). Selain itu, tanaman ini dilaporkan dapat menginisiasi penyembuhan luka, antibakteri, antivirus, dan antioksidan (Kusumawati et al, 2014;Sukmawati et al, 2019;Sapiun et al, 2019;Marlina et al, 2021;Tarigan et al, 2021). Pengetahuan masyarakat terhadap tumbuhan iler dan manfaatnya sebagai herbal, masih sangat rendah.…”